PML Antibody (aa1-581) LS-C9654
LifeSpan Biosciences
catalog: LS-C9654

mouse monoclonal
reactivity: human, mouse
application: western blot, immunocytochemistry

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PML Antibody LS-C74991
LifeSpan Biosciences
catalog: LS-C74991

domestic rabbit polyclonal
reactivity: human
application: western blot

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PML Antibody LS-C74990
LifeSpan Biosciences
catalog: LS-C74990

domestic rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry

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PML Antibody (aa470-499) LS-C98465
LifeSpan Biosciences
catalog: LS-C98465

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section

Western blot of anti-PML Sumoylation Site Antibody in HeLa cell line lysates (35 ug/lane). PML (arrow) was detected using the purified antibody.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Confocal immunofluorescence of PML Sumoylation Site Antibody with HeLa cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
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IHC-plus™ PML Antibody (aa588-637) LS-B3501
LifeSpan Biosciences
catalog: LS-B3501

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Anti-PML antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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PML Antibody (aa35-84) LS-C110248
LifeSpan Biosciences
catalog: LS-C110248

domestic rabbit polyclonal
reactivity: human
application: western blot

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PML Antibody (C-Terminus) LS-C110435
LifeSpan Biosciences
catalog: LS-C110435

domestic rabbit polyclonal
reactivity: human
application: western blot

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PML Antibody (aa11-60) LS-C118558
LifeSpan Biosciences
catalog: LS-C118558

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Immunofluorescence analysis of A549 cells, using PML Antibody. The picture on the right is blocked with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human tonsil tissue, using PML Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from A549 cells, using PML Antibody. The lane on the right is blocked with the synthesized peptide.
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PML Antibody LS-C126143
LifeSpan Biosciences
catalog: LS-C126143

mouse monoclonal
reactivity: human
application: western blot

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PML Antibody (N-Terminus) LS-C144807
LifeSpan Biosciences
catalog: LS-C144807

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, immunohistochemistry

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PML Antibody (Pro39) LS-C176183
LifeSpan Biosciences
catalog: LS-C176183

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot of PML (P39) pAb in extracts from A549 cells.

IHC of PML (P39) pAb in paraffin-embedded human tonsil thyroid tissue.
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PML Antibody (clone 1B9) LS-C179770
LifeSpan Biosciences
catalog: LS-C179770

mouse monoclonal (1B9)
reactivity: human
application: western blot, immunocytochemistry, immunoprecipitation, flow cytometry

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PML Antibody LS-C179910
LifeSpan Biosciences
catalog: LS-C179910

domestic rabbit polyclonal
reactivity: human
application: immunocytochemistry, flow cytometry

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PML Antibody (clone 880C3a) LS-C184788
LifeSpan Biosciences
catalog: LS-C184788

mouse monoclonal (880C3a)
reactivity: human
application: immunocytochemistry

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PML Antibody (clone GF1) LS-C189892
LifeSpan Biosciences
catalog: LS-C189892

mouse monoclonal (GF1)
reactivity: human
application: western blot, ELISA

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PML Antibody (clone 1D12) LS-C197930
LifeSpan Biosciences
catalog: LS-C197930

mouse monoclonal (1D12)
reactivity: human
application: western blot, ELISA, immunocytochemistry

PML monoclonal antibody (M02), clone 1D12. Western Blot analysis of PML expression in PC-12.

Immunofluorescence of monoclonal antibody to PML on HeLa cell. [antibody concentration 10 ug/ml]

Detection limit for recombinant GST tagged PML is approximately 0.3 ng/ml as a capture antibody.
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PML Antibody (aa470-499, AP) LS-C220397
LifeSpan Biosciences
catalog: LS-C220397

domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA, immunohistochemistry

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PML Antibody (aa470-499, APC) LS-C220398
LifeSpan Biosciences
catalog: LS-C220398

domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA, immunohistochemistry

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PML Antibody (aa470-499, Biotin) LS-C220399
LifeSpan Biosciences
catalog: LS-C220399

domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA, immunohistochemistry

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PML Antibody (aa470-499, FITC) LS-C220400
LifeSpan Biosciences
catalog: LS-C220400

domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA, immunohistochemistry

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PML Antibody (aa470-499, Azide-free, HRP) LS-C220403
LifeSpan Biosciences
catalog: LS-C220403

domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA, immunohistochemistry

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PML Antibody (aa470-499, PE) LS-C220404
LifeSpan Biosciences
catalog: LS-C220404

domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA, immunohistochemistry

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PML Antibody (aa375-425) LS-C286490
LifeSpan Biosciences
catalog: LS-C286490

domestic rabbit polyclonal
reactivity: human
application: immunohistochemistry

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PML Antibody (aa375-425) LS-C287992
LifeSpan Biosciences
catalog: LS-C287992

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunoprecipitation

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PML Antibody (aa575-625) LS-C287994
LifeSpan Biosciences
catalog: LS-C287994

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunoprecipitation

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PML Antibody (aa59-239, Biotin) LS-C298159
LifeSpan Biosciences
catalog: LS-C298159

domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot

Western Blot; Sample: Human Liver lysate; Primary Ab: 2µg/mL Rabbit Anti-Human PML Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody

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PML Antibody (aa59-239, FITC) LS-C302681
LifeSpan Biosciences
catalog: LS-C302681

domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot

Western Blot; Sample: Human Liver lysate; Primary Ab: 2µg/mL Rabbit Anti-Human PML Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody

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PML Antibody LS-C192443
LifeSpan Biosciences
catalog: LS-C192443

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

Western blot of PML pAb in extracts from Hela cells.
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PML Antibody (aa470-499) LS-C328283
LifeSpan Biosciences
catalog: LS-C328283

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry

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PML Antibody (clone 880C3a) LS-C342803
LifeSpan Biosciences
catalog: LS-C342803

mouse monoclonal (880C3a)
reactivity: human
application: western blot, immunocytochemistry

Western blot analysis of immunized recombinant protein, using anti-PML monoclonal antibody.
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PML Antibody (N-Terminus) LS-C352680
LifeSpan Biosciences
catalog: LS-C352680

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot analysis of PML expression in K562 (A); COLO320 (B) whole cell lysates.

Immunohistochemical analysis of PML staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Immunofluorescent analysis of PML staining in COLO320 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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PML Antibody (30-110 aa, N-terminal) LS-C385584
LifeSpan Biosciences
catalog: LS-C385584

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry

Western blot of PML antibody
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