IHC-plus™ PLK1 / PLK-1 Antibody (phospho-Thr210) LS-B50
LifeSpan Biosciences
catalog: LS-B50
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section

Anti-PLK1 antibody IHC of human lung carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
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IHC-plus™ PLK1 / PLK-1 Antibody (phospho-Thr210, clone 2A3) LS-B1675
LifeSpan Biosciences
catalog: LS-B1675
mouse monoclonal (2A3)
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section
citations: 1

Anti-PLK1 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 15 ug/ml.

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IHC-plus™ PLK1 / PLK-1 Antibody LS-B4225
LifeSpan Biosciences
catalog: LS-B4225
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Sample(30 ug whole cell lysate). A: Raji. 7.5% SDS PAGE. PLK1 antibody diluted at 1:1000.

Anti-PLK1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

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PLK1 / PLK-1 Antibody (clone OTI3F12) LS-C114491
LifeSpan Biosciences
catalog: LS-C114491
mouse monoclonal (OTI3F12)
reactivity: human
application: western blot, immunohistochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section
citations: 1

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PLK1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PLK1.

IHC of paraffin-embedded liver tissue using anti-PLK1 mouse monoclonal antibody. (Dilution 1:50).

Immunoprecipitation(IP) of PLK1 by using monoclonal anti-PLK1 antibodies (Negative control: IP without adding anti-PLK1 antibody.). For each experiment, 500ul of DDK tagged PLK1 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2 ug of anti-PLK1 antibody and 20ul (0.1 mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
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PLK1 / PLK-1 Antibody LS-C9648
LifeSpan Biosciences
catalog: LS-C9648
mouse monoclonal
reactivity: human
application: western blot, ELISA, immunocytochemistry, immunoprecipitation
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PLK1 / PLK-1 Antibody LS-C9649
LifeSpan Biosciences
catalog: LS-C9649
mouse monoclonal
reactivity: human
application: western blot, ELISA, immunocytochemistry, immunoprecipitation
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PLK1 / PLK-1 Antibody (aa402-603) LS-C17320
LifeSpan Biosciences
catalog: LS-C17320
mouse monoclonal
reactivity: human
application: western blot, ELISA, immunocytochemistry, immunoprecipitation
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PLK1 / PLK-1 Antibody (clone 3F8) LS-C40938
LifeSpan Biosciences
catalog: LS-C40938
mouse monoclonal (3F8)
reactivity: human
application: western blot, immunoprecipitation

Hela cell extract (Lane 1) or NIH3T3 cell extract (Lane 2) was resolved by electrophoresis, transferred to nitrocellulose and probed with monoclonal anti-Plk-1 (Clone 3F8) antibody. Proteins were visualized using a goat anti-mouse secondary conjugated to.
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PLK1 / PLK-1 Antibody (Internal, clone 3F8) LS-C63154
LifeSpan Biosciences
catalog: LS-C63154
mouse monoclonal (3F8)
reactivity: human
application: western blot

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PLK1 / PLK-1 Antibody (aa573-603) LS-C99728
LifeSpan Biosciences
catalog: LS-C99728
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Western blot of anti-PLK antibody in A375 cell lysate. PLK (arrow) was detected using purified antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
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PLK1 / PLK-1 Antibody (aa176-225) LS-C117697
LifeSpan Biosciences
catalog: LS-C117697
domestic rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry

Immunofluorescence analysis of A549 cells, using PLK1 Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from LOVO cells, treated with PMA 125ng/ml 30', using PLK1 Antibody. The lane on the right is blocked with the synthesized peptide.
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IHC-plus™ PLK1 / PLK-1 Antibody (C-Terminus) LS-B5792
LifeSpan Biosciences
catalog: LS-B5792
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Anti-PLK1 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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PLK1 / PLK-1 Antibody (N-Terminus) LS-C146512
LifeSpan Biosciences
catalog: LS-C146512
domestic rabbit polyclonal
reactivity: human
application: western blot
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IHC-plus™ PLK1 / PLK-1 Antibody (aa100-200) LS-B6508
LifeSpan Biosciences
catalog: LS-B6508
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Anti-PLK1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
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IHC-plus™ PLK1 / PLK-1 Antibody LS-B6646
LifeSpan Biosciences
catalog: LS-B6646
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Anti-PLK1 antibody IHC of human colon, epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
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PLK1 / PLK-1 Antibody (clone 1D1) LS-C169107
LifeSpan Biosciences
catalog: LS-C169107
mouse monoclonal (1D1)
reactivity: human
application: western blot, ELISA, immunocytochemistry
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PLK1 / PLK-1 Antibody (clone 3C11) LS-C169110
LifeSpan Biosciences
catalog: LS-C169110
mouse monoclonal (3C11)
reactivity: human
application: western blot, ELISA, immunohistochemistry, flow cytometry
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PLK1 / PLK-1 Antibody (clone OTI1D4) LS-C114495
LifeSpan Biosciences
catalog: LS-C114495
mouse monoclonal (OTI1D4)
reactivity: human
application: western blot, immunohistochemistry, immunoprecipitation, immunohistochemistry - paraffin section

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PLK1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PLK1.

IHC of paraffin-embedded Adenocarcinoma of breast tissue using anti-PLK1 mouse monoclonal antibody. (Dilution 1:50).

Immunoprecipitation(IP) of PLK1 by using monoclonal anti-PLK1 antibodies (Negative control: IP without adding anti-PLK1 antibody.). For each experiment, 500ul of DDK tagged PLK1 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2 ug of anti-PLK1 antibody and 20ul (0.1 mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
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PLK1 / PLK-1 Antibody (clone OTI8C12) LS-C114499
LifeSpan Biosciences
catalog: LS-C114499
mouse monoclonal (OTI8C12)
reactivity: human
application: western blot, immunocytochemistry, immunoprecipitation, flow cytometry

Western blot of extracts (35 ug) from 9 different cell lines by using anti-PLK1 monoclonal antibody.

Anti-PLK1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PLK1.

Immunoprecipitation(IP) of PLK1 by using monoclonal anti-PLK1 antibodies (Negative control: IP without adding anti-PLK1 antibody.). For each experiment, 500ul of DDK tagged PLK1 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2 ug of anti-PLK1 antibody and 20ul (0.1 mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
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PLK1 / PLK-1 Antibody (clone OTI5C1) LS-C114502
LifeSpan Biosciences
catalog: LS-C114502
mouse monoclonal (OTI5C1)
reactivity: human
application: western blot, immunohistochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PLK1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PLK1.

IHC of paraffin-embedded colon tissue using anti-PLK1 mouse monoclonal antibody. (Dilution 1:50).

Immunoprecipitation(IP) of PLK1 by using monoclonal anti-PLK1 antibodies (Negative control: IP without adding anti-PLK1 antibody.). For each experiment, 500ul of DDK tagged PLK1 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2 ug of anti-PLK1 antibody and 20ul (0.1 mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
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PLK1 / PLK-1 Antibody (clone OTI6C6) LS-C114507
LifeSpan Biosciences
catalog: LS-C114507
mouse monoclonal (OTI6C6)
reactivity: human
application: western blot, immunohistochemistry, immunoprecipitation, immunohistochemistry - paraffin section

Western blot of extracts (35 ug) from 9 different cell lines by using anti-PLK1 monoclonal antibody.

IHC of paraffin-embedded colon tissue using anti-PLK1 mouse monoclonal antibody. (Dilution 1:50).

Immunoprecipitation(IP) of PLK1 by using monoclonal anti-PLK1 antibodies (Negative control: IP without adding anti-PLK1 antibody.). For each experiment, 500ul of DDK tagged PLK1 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2 ug of anti-PLK1 antibody and 20ul (0.1 mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
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PLK1 / PLK-1 Antibody (clone OTI9E9) LS-C172325
LifeSpan Biosciences
catalog: LS-C172325
mouse monoclonal (OTI9E9)
reactivity: human
application: western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PLK1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PLK1.
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PLK1 / PLK-1 Antibody (aa287-298) LS-C186577
LifeSpan Biosciences
catalog: LS-C186577
domestic goat polyclonal
reactivity: human
application: western blot

PLK1 antibody (0.3 ug/ml) staining of HeLa nuclear lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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PLK1 / PLK-1 Antibody (aa300-603, clone 1D4B) LS-C189750
LifeSpan Biosciences
catalog: LS-C189750
mouse monoclonal (1D4B)
reactivity: human
application: western blot, immunocytochemistry, immunoprecipitation
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PLK1 / PLK-1 Antibody (clone 2G12) LS-C197924
LifeSpan Biosciences
catalog: LS-C197924
mouse monoclonal (2G12)
reactivity: human
application: western blot, ELISA, immunocytochemistry, immunoprecipitation

Western blot analysis of PLK1 in extracts from 293T, HeLa and A549 cell using anti-PLK1 monoclonal antibody.

Immunofluorescence of monoclonal antibody to PLK1 on HeLa cell. [antibody concentration 10 ug/ml]

Immunoprecipitation of PLK1 transfected lysate using anti-PLK1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PLK1 rabbit polyclonal antibody.
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PLK1 / PLK-1 Antibody (phospho-Thr210) LS-C199481
LifeSpan Biosciences
catalog: LS-C199481
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: immunohistochemistry, immunohistochemistry - paraffin section

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using PLK1 (Phospho-Thr210) Antibody. The picture on the right is blocked with the phospho peptide.
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PLK1 / PLK-1 Antibody (aa573-603, AP) LS-C266556
LifeSpan Biosciences
catalog: LS-C266556
domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA, immunohistochemistry
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PLK1 / PLK-1 Antibody (aa573-603, APC) LS-C266557
LifeSpan Biosciences
catalog: LS-C266557
domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA, immunohistochemistry
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PLK1 / PLK-1 Antibody (aa573-603, Biotin) LS-C266558
LifeSpan Biosciences
catalog: LS-C266558
domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA, immunohistochemistry
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PLK1 / PLK-1 Antibody (aa573-603, FITC) LS-C266559
LifeSpan Biosciences
catalog: LS-C266559
domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA, immunohistochemistry
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PLK1 / PLK-1 Antibody (aa573-603, Azide-free, HRP) LS-C266560
LifeSpan Biosciences
catalog: LS-C266560
domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA, immunohistochemistry
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PLK1 / PLK-1 Antibody (aa573-603, PE) LS-C266566
LifeSpan Biosciences
catalog: LS-C266566
domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA, immunohistochemistry
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PLK1 / PLK-1 Antibody (aa325-375) LS-C286344
LifeSpan Biosciences
catalog: LS-C286344
domestic rabbit polyclonal
reactivity: human
application: immunocytochemistry
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PLK1 / PLK-1 Antibody (aa1-50) LS-C287162
LifeSpan Biosciences
catalog: LS-C287162
domestic rabbit polyclonal
reactivity: human
application: western blot, immunoprecipitation
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PLK1 / PLK-1 Antibody (aa325-375) LS-C287163
LifeSpan Biosciences
catalog: LS-C287163
domestic rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry, immunoprecipitation
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PLK1 / PLK-1 Antibody (phospho-Ser137) LS-C291191
LifeSpan Biosciences
catalog: LS-C291191
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot
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PLK1 / PLK-1 Antibody (aa573-603) LS-C324113
LifeSpan Biosciences
catalog: LS-C324113
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry
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PLK1 / PLK-1 Antibody LS-C332156
LifeSpan Biosciences
catalog: LS-C332156
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry

Western blot analysis of extracts of various cell lines.

Immunofluorescence analysis of U2OS cells.
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PLK1 / PLK-1 Antibody LS-C341284
LifeSpan Biosciences
catalog: LS-C341284
domestic rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry
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PLK1 / PLK-1 Antibody (phospho-Thr210) LS-C354059
LifeSpan Biosciences
catalog: LS-C354059
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Western blot analysis of PLK1 (pT210) expression in HeLa (A); A549 (B); K562 (C); COLO320 (D) whole cell lysates.

Immunohistochemical analysis of PLK1 (pT210) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
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PLK1 / PLK-1 Antibody (Internal) LS-C368472
LifeSpan Biosciences
catalog: LS-C368472
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot analysis of PLK1 expression in HeLa (A); mouse hippocampal homogenate (B) whole cell lysates.

Immunohistochemical analysis of PLK1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Immunofluorescent analysis of PLK1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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PLK1 / PLK-1 Antibody (80-160 aa) LS-C381030
LifeSpan Biosciences
catalog: LS-C381030
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, ELISA
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PLK1 / PLK-1 Antibody (150-230 aa) LS-C381555
LifeSpan Biosciences
catalog: LS-C381555
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, ELISA, immunohistochemistry

Western blot of Phospho-Plk (T210) antibody
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PLK1 / PLK-1 Antibody (150-230 aa) LS-C385588
LifeSpan Biosciences
catalog: LS-C385588
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry
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PLK1 / PLK-1 Antibody (80-160 aa) LS-C386836
LifeSpan Biosciences
catalog: LS-C386836
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA

Western blot of Plk1 antibody
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