IHC-plus™ PLA2G4A Antibody (aa153-166) LS-B5952
LifeSpan Biosciences
catalog: LS-B5952

domestic goat polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

PLA2G4A antibody (1µg/ml) staining of A549 cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-PLA2G4A antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.

PLA2G4A antibody flow cytometric analysis of paraformaldehyde fixed A549 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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cPLA2 Antibody (C-Terminus) LS-C17270
LifeSpan Biosciences
catalog: LS-C17270

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunoprecipitation

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PLA2G4A Antibody (phospho-Ser505) LS-C17275
LifeSpan Biosciences
catalog: LS-C17275

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, ELISA, immunoprecipitation

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IHC-plus™ PLA2G4A Antibody (aa471-520) LS-B7555
LifeSpan Biosciences
catalog: LS-B7555

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Anti-PLA2G4A antibody IHC of human tonsil, mitotic figures. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.

Immunofluorescence analysis of HUVEC cells, using c-PLA2 Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from HeLa cells, treated with TNF-a 20ng/ml 30', using c-PLA2 Antibody. The lane on the right is blocked with the synthesized peptide.
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PLA2G4A Antibody (aa513-541) LS-C163759
LifeSpan Biosciences
catalog: LS-C163759

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section

Western blot of lysate from 293 cell line, using PLA2G4A Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.

Formalin-fixed and paraffin-embedded human lung carcinoma with PLA2G4A Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Confocal immunofluorescence of PLA2G4A Antibody with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
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PLA2G4A Antibody (phospho-Ser505) LS-C192155
LifeSpan Biosciences
catalog: LS-C192155

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Western blot of p-cPLA2 (S505) pAb in extracts from NIH-3T3 cells treated with TNF-a.

Immunohistochemistry (IHC) analysis of p-cPLA2 (S505) pAb in paraffin-embedded human breast cancer tissue.
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PLA2G4A Antibody (clone 10G5) LS-C197915
LifeSpan Biosciences
catalog: LS-C197915

mouse monoclonal (10G5)
reactivity: human
application: western blot, ELISA

Detection limit for recombinant GST tagged PLA2G4A is approximately 0.3 ng/ml as a capture antibody.
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PLA2G4A Antibody (aa513-541, APC) LS-C226137
LifeSpan Biosciences
catalog: LS-C226137

domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA

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PLA2G4A Antibody (aa513-541, AP) LS-C226138
LifeSpan Biosciences
catalog: LS-C226138

domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA

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PLA2G4A Antibody (aa513-541, FITC) LS-C226139
LifeSpan Biosciences
catalog: LS-C226139

domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA

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PLA2G4A Antibody (aa513-541, Azide-free, HRP) LS-C226140
LifeSpan Biosciences
catalog: LS-C226140

domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA

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PLA2G4A Antibody (aa513-541, PE) LS-C226141
LifeSpan Biosciences
catalog: LS-C226141

domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA

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PLA2G4A Antibody (aa513-541, Biotin) LS-C227146
LifeSpan Biosciences
catalog: LS-C227146

domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA

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PLA2G4A Antibody (aa686-700) LS-C313173
LifeSpan Biosciences
catalog: LS-C313173

domestic rabbit polyclonal
reactivity: human
application: western blot

WB of PLA2G4 / PLA2G4A antibody. Lane 1: Rat Testis Tissue Lysate. Lane 2: Rat Brain Tissue Lysate. Lane 3: A549 Cell Lysate. Lane 4: COLO320 Cell Lysate.
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IHC-plus™ PLA2G4A Antibody (aa480-530) LS-B11332
LifeSpan Biosciences
catalog: LS-B11332

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Anti-cPLA2 antibody IHC staining of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.

Western blot anylsis of c-PLA2 pAb at a 1:500 dilution. Lane 1: HEK293T whoe cell lysate. Lane 2: Raw264.7 whole cell lysate. Lane 3: PC12 whole cell lysate.
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cPLA2 Antibody (aa563-749, clone C1) LS-C314247
LifeSpan Biosciences
catalog: LS-C314247

mouse monoclonal (C1)
reactivity: human
application: western blot

Western blot of recombinant PLA2G4A.

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PLA2G4A Antibody (aa513-541) LS-C330585
LifeSpan Biosciences
catalog: LS-C330585

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA

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PLA2G4A Antibody (phospho-Ser505) LS-C354045
LifeSpan Biosciences
catalog: LS-C354045

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot analysis of PLA2G4A (pS505) expression in HeLa lambda protein phosphatase-treated (A); NIH3T3 lambda protein phosphatase-treated (B) whole cell lysates.

Immunohistochemical analysis of PLA2G4A (pS505) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Immunofluorescent analysis of PLA2G4A (pS505) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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PLA2G4A Antibody (N-Terminus) LS-C354047
LifeSpan Biosciences
catalog: LS-C354047

domestic rabbit polyclonal
reactivity: human
application: western blot, immunoprecipitation

Western blot analysis of PLA2G4A expression in A549 (A); HeLa (B); mouse kidney (C) whole cell lysates.
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PLA2G4A Antibody (Internal) LS-C354050
LifeSpan Biosciences
catalog: LS-C354050

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot analysis of PLA2G4A expression in HeLa (A); THP1 (B); NIH3T3 (C) whole cell lysates.

Immunohistochemical analysis of PLA2G4A staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Immunofluorescent analysis of PLA2G4A staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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