NEFH / NF-H Antibody LS-C61817
LifeSpan Biosciences
catalog: LS-C61817

mouse monoclonal
reactivity: human, bovine
application: western blot, immunocytochemistry

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NEFH / NF-H Antibody LS-C2730
LifeSpan Biosciences
catalog: LS-C2730

chicken polyclonal
reactivity: human, bovine
application: western blot, immunocytochemistry

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NEFH / NF-H Antibody LS-C37564
LifeSpan Biosciences
catalog: LS-C37564

mouse monoclonal
reactivity: human, bovine
application: western blot, immunohistochemistry, immunohistochemistry - frozen section

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NEFH / NF-H Antibody LS-C37568
LifeSpan Biosciences
catalog: LS-C37568

mouse monoclonal
reactivity: human, bovine
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

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NEFH / NF-H Antibody LS-C61818
LifeSpan Biosciences
catalog: LS-C61818

chicken polyclonal
reactivity: human, bovine
application: western blot, immunocytochemistry

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NEFH / NF-H Antibody LS-C73366
LifeSpan Biosciences
catalog: LS-C73366

chicken polyclonal
reactivity: human, bovine
application: western blot, immunocytochemistry

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NEFH / NF-H Antibody LS-C73378
LifeSpan Biosciences
catalog: LS-C73378

chicken polyclonal
reactivity: human, bovine
application: western blot, ELISA, immunohistochemistry, immunocytochemistry

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NEFH / NF-H Antibody LS-C187452
LifeSpan Biosciences
catalog: LS-C187452

domestic rabbit polyclonal
reactivity: human, bovine
application: western blot, immunohistochemistry - paraffin section

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NEFH / NF-H Antibody LS-C190119
LifeSpan Biosciences
catalog: LS-C190119

domestic rabbit polyclonal
reactivity: human, bovine
application: western blot, immunohistochemistry

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NEFH / NF-H Antibody LS-C195145
LifeSpan Biosciences
catalog: LS-C195145

chicken polyclonal
reactivity: human, bovine
application: western blot, immunohistochemistry, immunocytochemistry

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NEFH / NF-H Antibody (clone AH1) LS-C204550
LifeSpan Biosciences
catalog: LS-C204550

mouse monoclonal (AH1)
reactivity: human, bovine
application: western blot, immunocytochemistry

Strip blots of crude rat spinal cord extract stained with three different antibodies to phosphorylated NF-H, MCA-NAP4 (lane 1), NEFH / NF-H antibody (Lane 2) and MCA-9B12 (lane 3). All three antibodies bind to a prominent band with an apparent SDS-PAGE molecular weight of 200kDa.

Mixed neuron/glial cultures stained with NEFH / NF-H antibody (green) and also stained with rabbit polyclonal antibody to neurofilament NF-L RPCA-NF-L (red). The NF-L antibody stains neurofilaments in both axons and dendrites, and so can reveal neuronal cell bodies, while NEFH / NF-H antibody binds to only heavily phosphorylated forms of NF-H which are localized to mature axonal neurofilaments. In this image a few axons course from left to right and top to bottom- since they contain both NF-L and phosphorylated NF-H they appear golden in color. Blue shows the distribution of DNA.
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NEFH / NF-H Antibody (clone 9B12) LS-C204552
LifeSpan Biosciences
catalog: LS-C204552

mouse monoclonal (9B12)
reactivity: human, bovine
application: western blot, immunocytochemistry

Strip blots of crude rat spinal cord extract stained with three different antibodies to phosphorylated NF-H, MCA-NAP4 (lane 1), MCA-AH1 (Lane 2) and NEFH / NF-H antibody (lane 3). All three antibodies bind to a prominent band with an apparent SDS-PAGE molecular weight of 200kDa.

Mixed neuron/glial cultures stained with NEFH / NF-H antibody (red) and rabbit GFAP antibody RPCA-GFAP (green). Axonal profiles are stained in red, while astrocytic cells are revealed in green. Nuclei are revealed with a fluorescent DNA stain (blue).
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NEFH / NF-H Antibody LS-C204553
LifeSpan Biosciences
catalog: LS-C204553

domestic rabbit polyclonal
reactivity: human, bovine
application: western blot, immunohistochemistry, immunocytochemistry

Lane labeled CBB shows Coomassie Brilliant Blue stained crude extract of rat spinal cord, with the prominent major neurofilament subunit indicated. In rodents, NF-H runs at 200kDa, NF-M at 145kDa and NF-L at 68kDa. The Rab lane shows a blot probed with rabbit anti NF-H antibody NEFH / NF-H antibody. A prominent band at an apparent SDS-PAGE molecular weight of 200kDa corresponds to phosphorylated form of NF-H. The lane marked Rab shows a similar blot probed with our rabbit antibody to NF-H, CPCA-NF-H.

Shows a section of rat spinal cord stained with NEFH / NF-H antibody (green) and monoclonal antibody to ubiquitin C-terminal hydrolase 1 (UCHL1) clone MCA-BH7 (red). The neurofilament NF-H antibody binds primarily to phosphorylated axonal forms of NF-H, and so stains axons coursing between the large UCHL1 positive neurons. These large cells are motorneurons and UCHL1 protein is a major component of the perikarya and dendrites of these cells.
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PathPlus™ NEFH / NF-H Antibody LS-B10015
LifeSpan Biosciences
catalog: LS-B10015

chicken polyclonal
reactivity: human, bovine
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Anti-NEFH / NF-H antibody IHC staining of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.
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