PFKM / PFK-1 Antibody (aa122-151) LS-C101264
LifeSpan Biosciences
catalog: LS-C101264
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Western blot of lysate from human skeletal muscle tissue lysate, using PFKM Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
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PFKM / PFK-1 Antibody (aa746-776) LS-C101265
LifeSpan Biosciences
catalog: LS-C101265
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot of PFKM polyclonal antibody in HeLa cell line lysate. PFKM(arrow) was detected using the purified antibody.

Formalin-fixed and paraffin-embedded human skeletal muscle reacted with PFKM Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Confocal immunofluorescence of Fructose 6 Phosphate Kinase (PFKM) Antibody with HeLa cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
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PFKM / PFK-1 Antibody LS-C59233
LifeSpan Biosciences
catalog: LS-C59233
domestic goat polyclonal
reactivity: human
application: western blot, ELISA
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PFKM / PFK-1 Antibody (C-Terminus) LS-C135936
LifeSpan Biosciences
catalog: LS-C135936
domestic rabbit polyclonal
reactivity: human
application: western blot
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PFKM / PFK-1 Antibody (clone 1D1) LS-C139191
LifeSpan Biosciences
catalog: LS-C139191
mouse monoclonal (1D1)
reactivity: human
application: western blot, ELISA

PFKM monoclonal antibody (M04), clone 1D1. Western blot of PFKM expression in K-562.

Detection limit for recombinant GST tagged PFKM is 0.3 ng/ml as a capture antibody.
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PFKM / PFK-1 Antibody LS-C147414
LifeSpan Biosciences
catalog: LS-C147414
domestic sheep polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry
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PFKM / PFK-1 Antibody (Biotin) LS-C147415
LifeSpan Biosciences
catalog: LS-C147415
domestic sheep polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA, immunohistochemistry, immunocytochemistry
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PFKM / PFK-1 Antibody LS-C147416
LifeSpan Biosciences
catalog: LS-C147416
domestic sheep polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry
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PFKM / PFK-1 Antibody (clone AT2F11) LS-C175824
LifeSpan Biosciences
catalog: LS-C175824
mouse monoclonal (AT2F11)
reactivity: human
application: western blot, ELISA

Western Blot: The HepG2 cell lysate (30 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PFKM antibody (1:500-1:2000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
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PFKM / PFK-1 Antibody (Lyophilized) LS-C209185
LifeSpan Biosciences
catalog: LS-C209185
domestic goat polyclonal
reactivity: human
application: western blot, ELISA, immunocytochemistry
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PFKM / PFK-1 Antibody (Lyophilized, HRP) LS-C209626
LifeSpan Biosciences
catalog: LS-C209626
domestic goat polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA
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PFKM / PFK-1 Antibody (Lyophilized, Biotin) LS-C209631
LifeSpan Biosciences
catalog: LS-C209631
domestic goat polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA
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PFKM / PFK-1 Antibody (aa122-151, APC) LS-C225508
LifeSpan Biosciences
catalog: LS-C225508
domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA, immunohistochemistry
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PFKM / PFK-1 Antibody (aa122-151, AP) LS-C225509
LifeSpan Biosciences
catalog: LS-C225509
domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA, immunohistochemistry
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PFKM / PFK-1 Antibody (aa122-151, Biotin) LS-C225510
LifeSpan Biosciences
catalog: LS-C225510
domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA, immunohistochemistry
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PFKM / PFK-1 Antibody (aa122-151, FITC) LS-C225511
LifeSpan Biosciences
catalog: LS-C225511
domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA, immunohistochemistry
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PFKM / PFK-1 Antibody (aa122-151, Azide-free, HRP) LS-C225512
LifeSpan Biosciences
catalog: LS-C225512
domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA, immunohistochemistry
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PFKM / PFK-1 Antibody (aa746-776, AP) LS-C225513
LifeSpan Biosciences
catalog: LS-C225513
domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA, immunohistochemistry
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PFKM / PFK-1 Antibody (aa122-151, PE) LS-C225514
LifeSpan Biosciences
catalog: LS-C225514
domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA, immunohistochemistry
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PFKM / PFK-1 Antibody (aa746-776, APC) LS-C225515
LifeSpan Biosciences
catalog: LS-C225515
domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA, immunohistochemistry
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PFKM / PFK-1 Antibody (aa746-776, Biotin) LS-C225516
LifeSpan Biosciences
catalog: LS-C225516
domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA, immunohistochemistry
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PFKM / PFK-1 Antibody (aa746-776, FITC) LS-C225517
LifeSpan Biosciences
catalog: LS-C225517
domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA, immunohistochemistry
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PFKM / PFK-1 Antibody (aa746-776, Azide-free, HRP) LS-C225518
LifeSpan Biosciences
catalog: LS-C225518
domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA, immunohistochemistry
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PFKM / PFK-1 Antibody (aa746-776, PE) LS-C225519
LifeSpan Biosciences
catalog: LS-C225519
domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA, immunohistochemistry
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PFKM / PFK-1 Antibody (aa1-50) LS-C290898
LifeSpan Biosciences
catalog: LS-C290898
domestic rabbit polyclonal
reactivity: human
application: western blot, immunoprecipitation
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PFKM / PFK-1 Antibody (aa730-780) LS-C290900
LifeSpan Biosciences
catalog: LS-C290900
domestic rabbit polyclonal
reactivity: human
application: western blot, immunoprecipitation
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IHC-plus™ PFKM / PFK-1 Antibody (aa1-253) LS-B10796
LifeSpan Biosciences
catalog: LS-B10796
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Sample (30 ug of whole cell lysate). A: A431 . 7.5% SDS PAGE. PFKM antibody diluted at 1:5000.

Anti-PFKM / PFK-1 antibody IHC staining of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 7.5 ug/ml.
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PFKM / PFK-1 Antibody (aa122-151) LS-C327407
LifeSpan Biosciences
catalog: LS-C327407
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry
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PFKM / PFK-1 Antibody (aa746-776) LS-C327410
LifeSpan Biosciences
catalog: LS-C327410
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry
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PFKM / PFK-1 Antibody LS-C334085
LifeSpan Biosciences
catalog: LS-C334085
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry

Western blot analysis of extracts of various cell lines.

Immunohistochemistry of paraffin-embedded human esophageal cancer tissue.
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PFKM / PFK-1 Antibody LS-C341634
LifeSpan Biosciences
catalog: LS-C341634
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry
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IHC-plus™ PFKM / PFK-1 Antibody (aa320-369) LS-B12566
LifeSpan Biosciences
catalog: LS-B12566
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Human Skeletal Muscle: Formalin-Fixed, Paraffin-Embedded (FFPE)

Western blot analysis of lysate from Hela cells, using PFK-1 antibody.
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PFKM / PFK-1 Antibody (Internal) LS-C358834
LifeSpan Biosciences
catalog: LS-C358834
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Western blot analysis of PFKM expression in SHSY5Y (A); A549 (B); Ramos (C) whole cell lysates.

Immunohistochemical analysis of PFKM staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
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PFKM / PFK-1 Antibody (aa2-390) LS-C373155
LifeSpan Biosciences
catalog: LS-C373155
domestic rabbit polyclonal
reactivity: human, rat
application: western blot

Western blot of PFKM / PFK-1 antibody.

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PFKM / PFK-1 Antibody (Internal) LS-C385467
LifeSpan Biosciences
catalog: LS-C385467
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry

Western blot of PFKM antibody
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