PAK1 Antibody (aa238-251) LS-C55227
LifeSpan Biosciences
catalog: LS-C55227
domestic goat polyclonal
reactivity: human
application: western blot

Antibody (1 ug/ml) staining of Rat Brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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PAK1 + PAK2 + PAK3 Antibody (phospho-Thr423) LS-C124333
LifeSpan Biosciences
catalog: LS-C124333
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: immunohistochemistry
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IHC-plus™ PAK1 Antibody LS-B4853
LifeSpan Biosciences
catalog: LS-B4853
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Sample (30 ug of whole cell lysate). A: A431. 7.5% SDS PAGE. PAK1 antibody diluted at 1:1000

Anti-PAK1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
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PAK1 + PAK2 + PAK3 Antibody LS-C124334
LifeSpan Biosciences
catalog: LS-C124334
domestic rabbit polyclonal
reactivity: human
application: immunohistochemistry
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PAK1 + PAK2 + PAK3 Antibody (Thr423) LS-C92994
LifeSpan Biosciences
catalog: LS-C92994
domestic rabbit polyclonal
reactivity: human
application: immunohistochemistry
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PAK1 Antibody (N-Terminus) LS-C38037
LifeSpan Biosciences
catalog: LS-C38037
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunoprecipitation, immunohistochemistry - paraffin section
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PAK1 Antibody (aa333-545) LS-C38039
LifeSpan Biosciences
catalog: LS-C38039
domestic rabbit polyclonal
reactivity: human
application: western blot, immunoprecipitation
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PAK1 Antibody (Internal) LS-C38042
LifeSpan Biosciences
catalog: LS-C38042
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunoprecipitation
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PAK1 Antibody (N-Terminus) LS-C47062
LifeSpan Biosciences
catalog: LS-C47062
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA
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PAK1 Antibody LS-C48269
LifeSpan Biosciences
catalog: LS-C48269
domestic rabbit polyclonal
reactivity: human
application: western blot, immunoprecipitation
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PAK1 Antibody (phospho-Thr423) LS-C96754
LifeSpan Biosciences
catalog: LS-C96754
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: immunocytochemistry

Confocal immunofluorescence of Phospho-PAK1-pT423 Antibody with HeLa cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Dot blot of anti-PAK1-pT423 Phospho-specific antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
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PAK1 Antibody (phospho-Thr212) LS-C97035
LifeSpan Biosciences
catalog: LS-C97035
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot

Dot blot of Phospho-PAK1-T212 antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
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PAK1 Antibody (Thr423) LS-C99498
LifeSpan Biosciences
catalog: LS-C99498
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot of PAK1 (arrow) using rabbit polyclonal PAK1 Antibody (T423). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PAK1 gene (Lane 2) (Origene Technologies).

Confocal immunofluorescence of PAK1 Antibody (T423) with HeLa cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).

Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with PAK1 Antibody (T423) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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PAK1 / PAK2 Antibody (phospho-Ser199/Ser204) LS-C74032
LifeSpan Biosciences
catalog: LS-C74032
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot
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PAK1 / PAK2 Antibody (phospho-Ser144/Ser141) LS-C74034
LifeSpan Biosciences
catalog: LS-C74034
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot
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PAK1 + PAK2 + PAK3 Antibody (C-Terminus) LS-C74039
LifeSpan Biosciences
catalog: LS-C74039
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA
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PAK1 + PAK2 + PAK3 Antibody (phospho-Thr423) LS-C74040
LifeSpan Biosciences
catalog: LS-C74040
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot
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PAK1 + PAK2 + PAK3 Antibody (phospho-Thr423/Thr402/Thr421) LS-C74041
LifeSpan Biosciences
catalog: LS-C74041
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot
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PAK1 + PAK2 + PAK3 Antibody (phospho-Thr423/Thr402/Thr436) LS-C74043
LifeSpan Biosciences
catalog: LS-C74043
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: ELISA
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PAK1 + PAK2 + PAK3 Antibody (phospho-Ser144/Ser141/Ser139) LS-C74044
LifeSpan Biosciences
catalog: LS-C74044
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot
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PAK1 Antibody (aa170-219) LS-C118317
LifeSpan Biosciences
catalog: LS-C118317
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using PAK1 Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from NIH/3T3 cells, treated with UV 15', using PAK1 Antibody. The lane on the right is blocked with the synthesized peptide.
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PAK1 Antibody (phospho-Thr212) LS-C124331
LifeSpan Biosciences
catalog: LS-C124331
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry
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PAK1 Antibody LS-C124332
LifeSpan Biosciences
catalog: LS-C124332
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry
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PAK1 Antibody (clone 4D1) LS-C133360
LifeSpan Biosciences
catalog: LS-C133360
mouse monoclonal (4D1)
reactivity: human
application: western blot, ELISA, immunocytochemistry

PAK1 monoclonal antibody (M02), clone 4D1 Western blot of PAK1 expression in HeLa NE.

Immunofluorescence of monoclonal antibody to PAK1 on HeLa cell. [antibody concentration 35 ug/ml]

Detection limit for recombinant GST tagged PAK1 is approximately 0.1 ng/ml as a capture antibody.
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IHC-plus™ PAK1 Antibody (clone 1E11) LS-B6113
LifeSpan Biosciences
catalog: LS-B6113
mouse monoclonal (1E11)
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section

Detection limit for recombinant GST tagged PAK1 is approximately 0.03 ng/ml as a capture antibody.

Anti-PAK1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
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IHC-plus™ PAK1 Antibody (aa178-227) LS-B7619
LifeSpan Biosciences
catalog: LS-B7619
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Anti-PAK1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.

Immunofluorescence analysis of HeLa cells, using PAK1 Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from 293 cells, treated with Etoposide 25uM 60', using PAK1 Antibody. The lane on the right is blocked with the synthesized peptide.
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IHC-plus™ PAK1 Antibody (aa165-214) LS-B7662
LifeSpan Biosciences
catalog: LS-B7662
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Anti-PAK1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.

Immunofluorescence analysis of HeLa cells, using PAK1/2 Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from K562 cells, using PAK1/2 Antibody. The lane on the right is blocked with the synthesized peptide.
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PAK1 Antibody (Ile206) LS-C176444
LifeSpan Biosciences
catalog: LS-C176444
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot of PAK1 (I206) pAb in extracts from 293 cells treated with Etoposide 25uM 60'.

IHC of PAK1 (I206) pAb in paraffin-embedded human colon carcinoma tissue.
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PAK1 Antibody (Glu195) LS-C176642
LifeSpan Biosciences
catalog: LS-C176642
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot of PAK (E195) pAb in extracts from K562 cells.

IHC of PAK (E195) pAb in paraffin-embedded human breast carcinoma tissue.
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PAK1 Antibody (Lys198) LS-C177783
LifeSpan Biosciences
catalog: LS-C177783
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Western blot of PAK (K198) pAb in extracts from 3T3 cells.

IHC of PAK (K198) pAb in paraffin-embedded human brain tissue.
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PAK1 Antibody (phospho-Ser199) LS-C199397
LifeSpan Biosciences
catalog: LS-C199397
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Immunohistochemistry analysis of paraffin-embedded human placenta, using PAK1 (Phospho-Ser199) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from LOVO cells treated with starved 24h, using PAK1 (Phospho-Ser199) Antibody. The lane on the right is blocked with the phospho peptide.
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PAK1 Antibody (phospho-Ser204) LS-C199398
LifeSpan Biosciences
catalog: LS-C199398
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Immunohistochemistry analysis of paraffin-embedded human brain, using PAK1 (Phospho-Ser204) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from NIH/3T3 cells treated with UV 15', using PAK1 (Phospho-Ser204) Antibody. The lane on the right is blocked with the phospho peptide.
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PAK1 Antibody (phospho-Thr212) LS-C199399
LifeSpan Biosciences
catalog: LS-C199399
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using PAK1 (Phospho-Thr212) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from 293 cells treated with etoposide 25uM 1h, using PAK1 (Phospho-Thr212) Antibody. The lane on the right is blocked with the phospho peptide.
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PAK1 Antibody (aa149-198) LS-C200458
LifeSpan Biosciences
catalog: LS-C200458
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

Western blot analysis of the lysates from COLO205 cells using PAK1 antibody.
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PAK1 Antibody (phospho-Thr212, AP) LS-C280500
LifeSpan Biosciences
catalog: LS-C280500
domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA
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PAK1 Antibody (phospho-Thr212, APC) LS-C280501
LifeSpan Biosciences
catalog: LS-C280501
domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA
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PAK1 Antibody (phospho-Thr212, Biotin) LS-C280502
LifeSpan Biosciences
catalog: LS-C280502
domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA
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PAK1 Antibody (phospho-Thr212, FITC) LS-C280503
LifeSpan Biosciences
catalog: LS-C280503
domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA
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PAK1 Antibody (phospho-Thr212, Azide-free, HRP) LS-C280504
LifeSpan Biosciences
catalog: LS-C280504
domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA
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PAK1 Antibody (phospho-Thr212, PE) LS-C280505
LifeSpan Biosciences
catalog: LS-C280505
domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA
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PAK1 Antibody (phospho-Thr423, AP) LS-C280506
LifeSpan Biosciences
catalog: LS-C280506
domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: ELISA
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PAK1 Antibody (phospho-Thr423, APC) LS-C280507
LifeSpan Biosciences
catalog: LS-C280507
domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: ELISA
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PAK1 Antibody (phospho-Thr423, Biotin) LS-C280508
LifeSpan Biosciences
catalog: LS-C280508
domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: ELISA
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PAK1 Antibody (phospho-Thr423, FITC) LS-C280509
LifeSpan Biosciences
catalog: LS-C280509
domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: ELISA
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PAK1 Antibody (phospho-Thr423, Azide-free, HRP) LS-C280510
LifeSpan Biosciences
catalog: LS-C280510
domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: ELISA
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PAK1 Antibody (phospho-Thr423, PE) LS-C280511
LifeSpan Biosciences
catalog: LS-C280511
domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: ELISA
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PAK1 Antibody (Thr423, AP) LS-C280512
LifeSpan Biosciences
catalog: LS-C280512
domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA, immunohistochemistry
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PAK1 Antibody (Thr423, APC) LS-C280513
LifeSpan Biosciences
catalog: LS-C280513
domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA, immunohistochemistry
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PAK1 Antibody (Thr423, Biotin) LS-C280514
LifeSpan Biosciences
catalog: LS-C280514
domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA, immunohistochemistry
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PAK1 Antibody (Thr423, PE) LS-C280517
LifeSpan Biosciences
catalog: LS-C280517
domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA, immunohistochemistry
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PAK1 Antibody (aa40-90) LS-C288071
LifeSpan Biosciences
catalog: LS-C288071
domestic rabbit polyclonal
reactivity: human
application: immunoprecipitation
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PAK1 Antibody (aa1-50) LS-C288072
LifeSpan Biosciences
catalog: LS-C288072
domestic rabbit polyclonal
reactivity: human
application: western blot, immunoprecipitation
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PAK1 Antibody (C-Terminus) LS-C305969
LifeSpan Biosciences
catalog: LS-C305969
domestic rabbit polyclonal
reactivity: human
application: western blot
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PAK1 Antibody (aa1-14) LS-C312721
LifeSpan Biosciences
catalog: LS-C312721
domestic rabbit polyclonal
reactivity: human
application: western blot

WB of PAK1 antibody. Lane 1: Rat Testis Tissue Lysate. Lane 2: Rat Spleen Tissue Lysate. Lane 3: SW620 Cell Lysate. Lane 4: MCF-7 Cell Lysate. Lane 5: CEM Cell Lysate. Lane 6: HT1080 Cell Lysate.
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PAK1 Antibody (Thr423) LS-C328132
LifeSpan Biosciences
catalog: LS-C328132
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry
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PAK1 Antibody (phospho-Thr423) LS-C328134
LifeSpan Biosciences
catalog: LS-C328134
domestic rabbit polyclonal
reactivity: human
application: ELISA
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PAK1 Antibody (phospho-Thr212) LS-C330575
LifeSpan Biosciences
catalog: LS-C330575
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA
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PAK1 Antibody LS-C332162
LifeSpan Biosciences
catalog: LS-C332162
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

Western blot analysis of extracts of mouse kidney cells.

Immunohistochemistry of paraffin-embedded rat kidney tissue.
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PAK1 Antibody LS-C332460
LifeSpan Biosciences
catalog: LS-C332460
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

Western blot analysis of extracts of 293T cells.

Immunohistochemistry of paraffin-embedded human liver cancer tissue.
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PAK1 Antibody (phospho-Thr212) LS-C335853
LifeSpan Biosciences
catalog: LS-C335853
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry

Western blot analysis of extracts from 293 cells.

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue.

Immunofluorescence staining of methanol-fixed Hela cells.
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PAK1 Antibody (phospho-Thr423/402/421) LS-C336060
LifeSpan Biosciences
catalog: LS-C336060
domestic rabbit polyclonal
reactivity: human
application: immunohistochemistry, immunocytochemistry

Immunohistochemistry of paraffin-embedded human breast carcinoma tissue.

Immunofluorescence of methanol-fixed HeLa cells.
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PAK1 Antibody (Internal) LS-C356243
LifeSpan Biosciences
catalog: LS-C356243
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot analysis of PAK1 expression in A431 (A); HEK293T (B) whole cell lysates.

Immunohistochemical analysis of PAK1 staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Immunofluorescent analysis of PAK1 staining in HEK293T cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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PAK1 Antibody (phospho-Thr212) LS-C356247
LifeSpan Biosciences
catalog: LS-C356247
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot analysis of PAK1 (pT212) expression in HCT116 (A) whole cell lysates.

Immunohistochemical analysis of PAK1 (pT212) staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Immunofluorescent analysis of PAK1 (pT212) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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PAK1 Antibody (phospho-Ser204) LS-C357282
LifeSpan Biosciences
catalog: LS-C357282
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Western blot of extracts from 3T3 cells, treated with UV (15mins), using PAK1 (Phospho-Ser204) antibody.

Immunohistochemistry of paraffin-embedded human brain tissue using PAK1 (Phospho-Ser204) antibody.
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PAK1 Antibody (aa1-240) LS-C377326
LifeSpan Biosciences
catalog: LS-C377326
domestic rabbit polyclonal
reactivity: human
application: ELISA, immunohistochemistry, immunohistochemistry - paraffin section

Immunohistochemistry of paraffin-embedded human endometrial cancer using antibody at 1:100 dilution.
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PAK1 Antibody (150-230 aa) LS-C381263
LifeSpan Biosciences
catalog: LS-C381263
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, ELISA, immunohistochemistry

Western blot of Phospho-PAK alpha (T212) antibody
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PAK1 Antibody (140-220 aa) LS-C381355
LifeSpan Biosciences
catalog: LS-C381355
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, ELISA, immunohistochemistry
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PAK1 Antibody (140-220 aa) LS-C381390
LifeSpan Biosciences
catalog: LS-C381390
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, ELISA, immunohistochemistry

Western blot of Phospho-PAK alpha (S204) antibody
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PAK1 Antibody (140-220 aa) LS-C385348
LifeSpan Biosciences
catalog: LS-C385348
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry
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PAK1 Antibody (150-230 aa) LS-C385351
LifeSpan Biosciences
catalog: LS-C385351
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry

Western blot of PAK alpha antibody
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PAK1 Antibody (140-220 aa) LS-C385352
LifeSpan Biosciences
catalog: LS-C385352
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry

Western blot of PAK alpha antibody
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PAK1 Antibody (Internal) LS-C413063
LifeSpan Biosciences
catalog: LS-C413063
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot analysis of PAK1 expression in HeLa (A); HEK293T (B) whole cell lysates.

Immunohistochemical analysis of PAK1 staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Immunofluorescent analysis of PAK1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
quantity:
price:
to the supplier