IHC-plus™ ACO2 / Aconitase 2 Antibody (aa438-467) LS-B3427
LifeSpan Biosciences
catalog: LS-B3427

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Anti-ACO2 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

Perfused isolated rat heart whole tissue lysate was lysed with either A) 50 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, 1% NP-40, 0.1% SDS, 0.5% Na-deoxycholate, 1 mM Na3VO4, 20 mM NaF, 1 mM PMSF, 5 v/v % protease inhibitor cocktail or B) T-PER Tissue Protein Extraction Reagent [# 785101; Pierce], containing 1mM Na3VO4, 20 mM NaF, 5 v/v % protease inhibitor cocktail (Sigma); PVDF membrane was incubated in primary Ab [rabbit polyclonal antibody against ACO2. Solution: 1:1000 diluted in 5% NFM TBS-T 0,05 for overnight (15 hrs) at 4 ?. Data courtesy of Boglarka Laczy M.D., Division of Cardiovascular Disease, Dept. of Medicine, University of Alabama at Birmingham.
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ACO2 / Aconitase 2 Antibody (aa294-325) LS-C100181
LifeSpan Biosciences
catalog: LS-C100181

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Western blot of ACO2 (arrow) using rabbit polyclonal ACO2 Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ACO2 gene (Lane 2) (Origene Technologies).

Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with ACO2 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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ACO2 / Aconitase 2 Antibody (aa108-157) LS-C110898
LifeSpan Biosciences
catalog: LS-C110898

domestic rabbit polyclonal
reactivity: human
application: western blot

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IHC-plus™ ACO2 / Aconitase 2 Antibody (aa554-566) LS-B8319
LifeSpan Biosciences
catalog: LS-B8319

domestic goat polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

ACO2 antibody (0.01 ug/ml) staining of Heart lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-ACO2 / Aconitase 2 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 3.75 ug/ml.
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IHC-plus™ ACO2 / Aconitase 2 Antibody (aa541-555) LS-B8320
LifeSpan Biosciences
catalog: LS-B8320

domestic goat polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Anti-ACO2 / Aconitase 2 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 3.75 ug/ml.
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ACO2 / Aconitase 2 Antibody (clone OTI3G8) LS-C114898
LifeSpan Biosciences
catalog: LS-C114898

mouse monoclonal (OTI3G8)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACO2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ACO2.

IHC of paraffin-embedded Adenocarcinoma of breast tissue using anti-ACO2 mouse monoclonal antibody. (Dilution 1:50).

Anti-ACO2 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ACO2.
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ACO2 / Aconitase 2 Antibody (clone OTI7A11) LS-C114914
LifeSpan Biosciences
catalog: LS-C114914

mouse monoclonal (OTI7A11)
reactivity: human
application: western blot, immunocytochemistry, flow cytometry

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACO2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ACO2.

Anti-ACO2 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ACO2.

HEK293T cells transfected with either pCMV6-ENTRY ACO2 (Red) or empty vector control plasmid (Blue) were immunostained with anti-ACO2 mouse monoclonal, and then analyzed by flow cytometry.
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ACO2 / Aconitase 2 Antibody (clone OTI7G4) LS-C114938
LifeSpan Biosciences
catalog: LS-C114938

mouse monoclonal (OTI7G4)
reactivity: human
application: western blot, immunocytochemistry, flow cytometry

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACO2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ACO2.

Anti-ACO2 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ACO2.

HEK293T cells transfected with either pCMV6-ENTRY ACO2 (Red) or empty vector control plasmid (Blue) were immunostained with anti-ACO2 mouse monoclonal, and then analyzed by flow cytometry.
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ACO2 / Aconitase 2 Antibody (clone OTI3G10) LS-C114971
LifeSpan Biosciences
catalog: LS-C114971

mouse monoclonal (OTI3G10)
reactivity: human
application: immunocytochemistry, flow cytometry

Anti-ACO2 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ACO2.

HEK293T cells transfected with either pCMV6-ENTRY ACO2 (Red) or empty vector control plasmid (Blue) were immunostained with anti-ACO2 mouse monoclonal, and then analyzed by flow cytometry.
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ACO2 / Aconitase 2 Antibody (clone 1A11) LS-C196601
LifeSpan Biosciences
catalog: LS-C196601

mouse monoclonal (1A11)
reactivity: human
application: ELISA

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ACO2 / Aconitase 2 Antibody (aa438-467, AP) LS-C274819
LifeSpan Biosciences
catalog: LS-C274819

domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA, immunohistochemistry

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ACO2 / Aconitase 2 Antibody (aa438-467, APC) LS-C274821
LifeSpan Biosciences
catalog: LS-C274821

domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA, immunohistochemistry

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ACO2 / Aconitase 2 Antibody (aa438-467, Biotin) LS-C274822
LifeSpan Biosciences
catalog: LS-C274822

domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA, immunohistochemistry

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ACO2 / Aconitase 2 Antibody (aa438-467, FITC) LS-C274823
LifeSpan Biosciences
catalog: LS-C274823

domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA, immunohistochemistry

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ACO2 / Aconitase 2 Antibody (aa438-467, Azide-free, HRP) LS-C274824
LifeSpan Biosciences
catalog: LS-C274824

domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA, immunohistochemistry

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ACO2 / Aconitase 2 Antibody (aa438-467, PE) LS-C274825
LifeSpan Biosciences
catalog: LS-C274825

domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA, immunohistochemistry

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ACO2 / Aconitase 2 Antibody LS-C309297
LifeSpan Biosciences
catalog: LS-C309297

domestic rabbit polyclonal
reactivity: human
application: western blot

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ACO2 / Aconitase 2 Antibody (aa438-467) LS-C326775
LifeSpan Biosciences
catalog: LS-C326775

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry

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