IHC-plus™ NUMA1 / NUMA Antibody (aa900-950) LS-B11047
LifeSpan Biosciences
catalog: LS-B11047

domestic rabbit polyclonal
reactivity: human
application: immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
citations: 1

Anti-NUMA1 / NUMA antibody IHC staining of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.

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NUMA1 / NUMA Antibody (aa1586-1635) LS-C81567
LifeSpan Biosciences
catalog: LS-C81567

domestic rabbit polyclonal
reactivity: human
application: western blot

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NUMA1 / NUMA Antibody (clone B497) LS-C171016
LifeSpan Biosciences
catalog: LS-C171016

mouse monoclonal (B497)
reactivity: human
application: immunohistochemistry, immunohistochemistry - paraffin section

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NUMA1 / NUMA Antibody (clone C02142) LS-C179470
LifeSpan Biosciences
catalog: LS-C179470

mouse monoclonal (C02142)
reactivity: human
application: western blot, immunocytochemistry, immunoprecipitation

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NUMA1 / NUMA Antibody (aa900-950) LS-C288302
LifeSpan Biosciences
catalog: LS-C288302

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

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NUMA1 / NUMA Antibody (aa2065-2115) LS-C288305
LifeSpan Biosciences
catalog: LS-C288305

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunoprecipitation

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IHC-plus™ NUMA1 / NUMA Antibody (aa1-339) LS-B10896
LifeSpan Biosciences
catalog: LS-B10896

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

NuMA antibody [N1], N-term detects NUMA1 protein by Western blot analysis. A. 30 ug HeLa whole cell lysate/extract. B. 30 ug A375 whole cell lysate/extract. 5 % SDS-PAGE. NuMA antibody [N1], N-term dilution:1:1000

NuMA antibody [N1], N-term Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed U2OS using NuMA antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with alpha-tubulin antibody (Red) at 1:2000. Scale bar = 10 m.

Anti-NUMA1 / NUMA antibody IHC staining of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 7.5 ug/ml.
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IHC-plus™ NUMA1 / NUMA Antibody (aa315-364) LS-B11432
LifeSpan Biosciences
catalog: LS-B11432

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Anti-NUMA1 / NUMA antibody IHC staining of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.

Western blot analysis of the lysates from K562 cells using NUMA1 antibody.
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NUMA1 / NUMA Antibody LS-C330989
LifeSpan Biosciences
catalog: LS-C330989

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry

Western blot analysis of extracts of various cell lines, using NUMA1 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90s.

Immunohistochemistry of paraffin-embedded human stomach using NUMA1 antibodyat dilution of 1:100 (40x lens).

Immunofluorescence analysis of U2OS cells using NUMA1 antibodyat dilution of 1:100. Blue: DAPI for nuclear staining.
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IHC-plus™ NUMA1 / NUMA Antibody (clone 1C5) LS-B11675
LifeSpan Biosciences
catalog: LS-B11675

mouse monoclonal (1C5)
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunoprecipitation, immunohistochemistry - paraffin section

NUMA1 monoclonal antibody (M01), clone 1C5 Western Blot analysis of NUMA1 expression in K-562.

Immunoprecipitation of NUMA1 transfected lysate using anti-NUMA1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with NUMA1 rabbit polyclonal antibody.

Detection limit for recombinant GST tagged NUMA1 is approximately 0.1 ng/ml as a capture antibody.
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NUMA1 / NUMA Antibody (N-Terminus) LS-C368913
LifeSpan Biosciences
catalog: LS-C368913

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Western blot analysis of NUMA expression in HeLa (A); A431 (B); A549 (C) whole cell lysates.

Immunohistochemical analysis of NUMA staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected with HRP and DAB as chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
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NUMA1 / NUMA Antibody (aa1700-2115) LS-C372941
LifeSpan Biosciences
catalog: LS-C372941

domestic rabbit polyclonal
reactivity: human
application: western blot

Western Blot; Sample: Human A431 cell lysate; Primary Ab: 3µg/ml Rabbit Anti-Human NUMA1 Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody

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