IHC-plus™ NUCB1 / Nucleobindin Antibody (C-Terminus) LS-B1551
LifeSpan Biosciences
catalog: LS-B1551

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Anti-NUCB1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

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NUCB1 / Nucleobindin Antibody (C-Terminus) LS-C31089
LifeSpan Biosciences
catalog: LS-C31089

domestic rabbit polyclonal
reactivity: human, rat
application: western blot, immunohistochemistry

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NUCB1 / Nucleobindin Antibody (C-Terminus) LS-C31094
LifeSpan Biosciences
catalog: LS-C31094

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation

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NUCB1 / Nucleobindin Antibody (clone OTI1A5) LS-C173489
LifeSpan Biosciences
catalog: LS-C173489

mouse monoclonal (OTI1A5)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NUCB1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NUCB1.

IHC of paraffin-embedded Human endometrium tissue using anti-NUCB1 mouse monoclonal antibody.

Anti-NUCB1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NUCB1.
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NUCB1 / Nucleobindin Antibody (clone OTI2A5) LS-C173523
LifeSpan Biosciences
catalog: LS-C173523

mouse monoclonal (OTI2A5)
reactivity: human
application: western blot, immunocytochemistry

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NUCB1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NUCB1.

Anti-NUCB1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NUCB1.
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NUCB1 / Nucleobindin Antibody (clone OTI1G3) LS-C173595
LifeSpan Biosciences
catalog: LS-C173595

mouse monoclonal (OTI1G3)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NUCB1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NUCB1.

IHC of paraffin-embedded Carcinoma of Human kidney tissue using anti-NUCB1 mouse monoclonal antibody.

Anti-NUCB1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NUCB1.
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NUCB1 / Nucleobindin Antibody (clone OTI1A3) LS-C337551
LifeSpan Biosciences
catalog: LS-C337551

mouse monoclonal (OTI1A3)
reactivity: human
application: western blot, flow cytometry

Western blot of extracts (35ug) from 9 different cell lines by using anti-NUCB1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
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IHC-plus™ NUCB1 / Nucleobindin Antibody (clone 1H8-2C11) LS-B13070
LifeSpan Biosciences
catalog: LS-B13070

mouse monoclonal (1H8-2C11)
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

NUCB1 monoclonal antibody (M01), clone 1H8-2C11 Western blot of NUCB1 expression in HepG2.

Immunofluorescence of monoclonal antibody to NUCB1 on HeLa cell. [antibody concentration 10 ug/ml]

Detection limit for recombinant GST tagged NUCB1 is approximately 0.1 ng/ml as a capture antibody.
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