IHC-plus™ YBX1 / YB1 Antibody (aa143-192) LS-B5607
LifeSpan Biosciences
catalog: LS-B5607
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Anti-YBX1 / YB1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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YBX1 / YB1 Antibody (C-Terminus) LS-C122232
LifeSpan Biosciences
catalog: LS-C122232
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
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YBX1 / YB1 Antibody (aa236-285) LS-C29829
LifeSpan Biosciences
catalog: LS-C29829
domestic rabbit polyclonal
reactivity: human
application: western blot
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YBX1 / YB1 Antibody (aa58-87) LS-C100023
LifeSpan Biosciences
catalog: LS-C100023
domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot of YBX1 (arrow) using rabbit polyclonal YBX1 Antibody (RB14116). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the YBX1 gene (Lane 2) (Origene Technologies).
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YBX1 / YB1 Antibody (aa276-305) LS-C100024
LifeSpan Biosciences
catalog: LS-C100024
domestic rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry

Western blot of YBX1(arrow) using rabbit polyclonal YBX1 Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the YBX1 gene (Lane 2) (Origene Technologies).

Fluorescent confocal image of U251 cell stained with YBX1 Antibody. U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with YBX1 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). YBX1 immunoreactivity is localized to Cytoplasm significantly.
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YBX1 / YB1 Antibody (N-Terminus) LS-C144945
LifeSpan Biosciences
catalog: LS-C144945
domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, immunohistochemistry
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YBX1 / YB1 Antibody (aa149-178) LS-C157043
LifeSpan Biosciences
catalog: LS-C157043
domestic rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry

YBX1 Antibody western blot of 293T cell line lysates (35 ug/lane). The YBX1 antibody detected the YBX1 protein (arrow).

Fluorescent image of A549 cell stained with YBX1 Antibody. A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with YBX1 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). YBX1 immunoreactivity is localized to Cytoplasm significantly.
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YBX1 / YB1 Antibody (aa68-117) LS-C199739
LifeSpan Biosciences
catalog: LS-C199739
domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot analysis of lysates from Jurkat cells, using YB1 Antibody. The lane on the right is blocked with the synthesized peptide.
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YBX1 / YB1 Antibody (phospho-Ser102) LS-C199740
LifeSpan Biosciences
catalog: LS-C199740
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot

Western blot analysis of lysates from HepG2 cells treated with PMA 125ng/ml 15', using YB1 (Phospho-Ser102) Antibody. The lane on the right is blocked with the phospho peptide.
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YBX1 / YB1 Antibody (aa201-250) LS-C205787
LifeSpan Biosciences
catalog: LS-C205787
domestic rabbit polyclonal
reactivity: human
application: western blot
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YBX1 / YB1 Antibody (aa149-178, AP) LS-C273778
LifeSpan Biosciences
catalog: LS-C273778
domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA
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YBX1 / YB1 Antibody (aa149-178, APC) LS-C273779
LifeSpan Biosciences
catalog: LS-C273779
domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA
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YBX1 / YB1 Antibody (aa149-178, Biotin) LS-C273780
LifeSpan Biosciences
catalog: LS-C273780
domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA
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YBX1 / YB1 Antibody (aa149-178, FITC) LS-C273781
LifeSpan Biosciences
catalog: LS-C273781
domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA
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YBX1 / YB1 Antibody (aa149-178, Azide-free, HRP) LS-C273782
LifeSpan Biosciences
catalog: LS-C273782
domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA
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YBX1 / YB1 Antibody (aa149-178, PE) LS-C273784
LifeSpan Biosciences
catalog: LS-C273784
domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA
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YBX1 / YB1 Antibody (aa137-152) LS-C312907
LifeSpan Biosciences
catalog: LS-C312907
domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot - Anti-YB1 Antibody
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IHC-plus™ YBX1 / YB1 Antibody (clone 4F12) LS-B11709
LifeSpan Biosciences
catalog: LS-B11709
mouse monoclonal (4F12)
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Immunofluorescence of monoclonal antibody to YBX1 on HeLa cell. [antibody concentration 20 ug/ml]

Detection limit for recombinant GST tagged YBX1 is approximately 0.3 ng/ml as a capture antibody.

Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
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YBX1 / YB1 Antibody (clone OTI2C9) LS-C339858
LifeSpan Biosciences
catalog: LS-C339858
mouse monoclonal (OTI2C9)
reactivity: human
application: western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY YBX1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-YBX1.
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YBX1 / YB1 Antibody (clone OTI7B4) LS-C339859
LifeSpan Biosciences
catalog: LS-C339859
mouse monoclonal (OTI7B4)
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY YBX1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-YBX1.

IHC of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-YBX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
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YBX1 / YB1 Antibody (clone OTI11A7) LS-C339912
LifeSpan Biosciences
catalog: LS-C339912
mouse monoclonal (OTI11A7)
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY YBX1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-YBX1.

IHC of paraffin-embedded Carcinoma of Human kidney tissue using anti-YBX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
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IHC-plus™ YBX1 / YB1 Antibody (aa224-274) LS-B12352
LifeSpan Biosciences
catalog: LS-B12352
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunoprecipitation, immunohistochemistry - paraffin section

Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
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IHC-plus™ YBX1 / YB1 Antibody (aa274-324) LS-B12353
LifeSpan Biosciences
catalog: LS-B12353
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunoprecipitation, immunohistochemistry - paraffin section

Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
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YBX1 / YB1 Antibody (phospho-Ser102) LS-C368798
LifeSpan Biosciences
catalog: LS-C368798
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot

Western blot analysis of YBX1 (pS102) expression in HeLa (A); MCF7 (B); Jurkat (C) whole cell lysates.
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YBX1 / YB1 Antibody (40-120 aa) LS-C381087
LifeSpan Biosciences
catalog: LS-C381087
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, ELISA
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YBX1 / YB1 Antibody (40-120 aa) LS-C386694
LifeSpan Biosciences
catalog: LS-C386694
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA
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