NRAS / N-ras Antibody (aa147-179) LS-C99435
LifeSpan Biosciences
catalog: LS-C99435

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, flow cytometry, immunohistochemistry - paraffin section

NRAS Antibody western blot of MCF-7 cell line lysates (35 ug/lane). The NRAS antibody detected the NRAS protein (arrow).

Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with NRAS antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

NRAS Antibody flow cytometry of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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IHC-plus™ Pan Ras Antibody (aa1-50) LS-B5494
LifeSpan Biosciences
catalog: LS-B5494

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Anti-Ras, Pan antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.

Western blot analysis of lysates from HeLa cells, using RASH/RASK Antibody. The lane on the right is blocked with the synthesized peptide.
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IHC-plus™ NRAS / N-ras Antibody (aa167-179) LS-B2501
LifeSpan Biosciences
catalog: LS-B2501

domestic goat polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

NRAS / N-ras antibody (0.1µg/ml) staining of Rat Intestines lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-NRAS antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
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IHC-plus™ NRAS / N-ras Antibody (clone AT2G9) LS-B6270
LifeSpan Biosciences
catalog: LS-B6270

mouse monoclonal (AT2G9)
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section

Extracts of 293T(35 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human RAS (1:500~1:5000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The cross-reacting of anti-human RAS(AT2G9) was analyzed by western blot with recombinant protein (200ng) of NRAS, KRAS and HRAS.

Anti-NRAS antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 15 ug/ml.
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IHC-plus™ NRAS / N-ras Antibody (aa91-140) LS-B8261
LifeSpan Biosciences
catalog: LS-B8261

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Human, Pancreas: Formalin-Fixed Paraffin-Embedded (FFPE). This image was taken for the unconjugated form of this product. Other forms have not been tested.
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NRAS / N-ras Antibody (clone OTI5G7) LS-C174539
LifeSpan Biosciences
catalog: LS-C174539

mouse monoclonal (OTI5G7)
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NRAS (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NRAS.

IHC of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-NRAS mouse monoclonal antibody.
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NRAS / N-ras Antibody (clone 2A3) LS-C197802
LifeSpan Biosciences
catalog: LS-C197802

mouse monoclonal (2A3)
reactivity: human
application: western blot, ELISA

Detection limit for recombinant GST tagged NRAS is approximately 0.1 ng/ml as a capture antibody.
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IHC-plus™ NRAS / N-ras Antibody LS-B10113
LifeSpan Biosciences
catalog: LS-B10113

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section

Western blot analysis of N-RAS in 293 cell lysate with N-RAS antibody at (A) 1 and (B) 2 ug/ml.

Anti-NRAS / N-ras antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
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NRAS / N-ras Antibody (aa70-101, clone 822CT17.5.1) LS-C344579
LifeSpan Biosciences
catalog: LS-C344579

mouse monoclonal (822CT17.5.1)
reactivity: human
application: western blot

NRAS Antibody western blot of A431 and rat C6 cell line lysates (35 ug/lane). The NRAS antibody detected the NRAS protein (arrow).
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NRAS / N-ras Antibody (clone RBT-NRAS, Ready-to-Use) LS-C348547
LifeSpan Biosciences
catalog: LS-C348547

domestic rabbit recombinant (RBT-NRAS)
reactivity: human
application: immunohistochemistry - paraffin section, immunohistochemistry - frozen section

IHC of NRAS on FFPE Melanoma Tissue. This image was taken for the unmodified form of this product. Other forms have not been tested.
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NRAS / N-ras Antibody (Q61R mutation, clone RBT-NRAS) LS-C348548
LifeSpan Biosciences
catalog: LS-C348548

domestic rabbit recombinant (RBT-NRAS)
reactivity: human
application: immunohistochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

IHC of NRAS on FFPE Melanoma Tissue.
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NRAS / N-ras Antibody LS-C356955
LifeSpan Biosciences
catalog: LS-C356955

domestic rabbit polyclonal
reactivity: human
application: western blot

All lanes : Anti-NRAS/HRAS/KRAS Antibody at 1:1000 dilution Lane 1: MCF-7 whole cell lysates Lane 2: HeLa whole cell lysates Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution Predicted band size : 21 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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