NME2 Antibody (aa25-54) LS-C101173
LifeSpan Biosciences
catalog: LS-C101173

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

NME2 Antibody (F40) western blot of HL-60,Jurkat,MCF-7 cell line,mouse kidney and rat stomach tissue lysates (35 ug/lane). The NME2 antibody detected the NME2 protein (arrow).

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Fluorescent confocal image of HeLa cell stained with NME2 Antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NME2 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). NME2 immunoreactivity is localized to Cytoplasm and Nucleus significantly.
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NME2 Antibody (clone 1D3) LS-C133349
LifeSpan Biosciences
catalog: LS-C133349

mouse monoclonal (1D3)
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

NME2 monoclonal antibody (M06), clone 1D3 Western blot of NME2 expression in HeLa.

Immunoperoxidase of monoclonal antibody to NME2 on formalin-fixed paraffin-embedded human testis. [antibody concentration 1.5 ug/ml]

Immunofluorescence of monoclonal antibody to NME2 on HeLa cell. [antibody concentration 10 ug/ml]
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IHC-plus™ NME2 Antibody (aa91-140) LS-B9880
LifeSpan Biosciences
catalog: LS-B9880

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section
citations: 1

Anti-NME2 / NM23 antibody IHC staining of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.

Western blot analysis of lysates from K562 cells, using NM23 Antibody. The lane on the right is blocked with the synthesized peptide.
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NME2 Antibody (His118) LS-C176292
LifeSpan Biosciences
catalog: LS-C176292

domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot of NM23/NDP Kinase B (H118) pAb in extracts from K562 cells.
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NME2 Antibody (aa25-54, AP) LS-C220189
LifeSpan Biosciences
catalog: LS-C220189

domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA, immunohistochemistry

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NME2 Antibody (aa25-54, Biotin) LS-C220191
LifeSpan Biosciences
catalog: LS-C220191

domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA, immunohistochemistry

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NME2 Antibody (aa25-54, APC) LS-C220192
LifeSpan Biosciences
catalog: LS-C220192

domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA, immunohistochemistry

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NME2 Antibody (aa25-54, FITC) LS-C220193
LifeSpan Biosciences
catalog: LS-C220193

domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA, immunohistochemistry

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NME2 Antibody (aa25-54, Azide-free, HRP) LS-C220194
LifeSpan Biosciences
catalog: LS-C220194

domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA, immunohistochemistry

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NME2 Antibody (aa25-54, PE) LS-C220195
LifeSpan Biosciences
catalog: LS-C220195

domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA, immunohistochemistry

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NME2 Antibody (aa137-152) LS-C312970
LifeSpan Biosciences
catalog: LS-C312970

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

WB of NME2 / NM23 antibody. All lanes: Anti-NME2 at 0.5ug/ml. Lane 1: Rat Heart Tissue Lysate at 40ug. Lane 2: Rat Brain Tissue Lysate at 40ug. Lane 3: Rat Liver Tissue Lysate at 40ug. Lane 4: Rat Skeletal Muscle Tissue Lysate at 40ug. Lane 5: PANC Whole Cell Lysate at 40ug. Lane 6: HELA Whole Cell Lysate at 40ug. Lane 7: SMMC Whole Cell Lysate at 40ug. Lane 8: U87 Whole Cell Lysate at 40ug. Lane 9: COLO320 Whole Cell Lysate at 40ug. Predicted bind size: 17KD. Observed bind size: 17KD.

NME2 / NM23 antibody. IHC(P): Rat Cerebellum Tissue.

NME2 / NM23 antibody. ICC: HELA Cell.
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NME2 Antibody (aa25-54) LS-C330559
LifeSpan Biosciences
catalog: LS-C330559

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry

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NME2 Antibody (clone OTI2F9) LS-C337918
LifeSpan Biosciences
catalog: LS-C337918

mouse monoclonal (OTI2F9)
reactivity: human
application: western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NME2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NME2.
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NME2 Antibody LS-C349144
LifeSpan Biosciences
catalog: LS-C349144

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry

Western blot analysis of extracts of various cell lines.

Immunohistochemistry of paraffin-embedded human kidney tissue.

Immunofluorescence analysis of A549 cells.
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NME2 Antibody (Internal) LS-C352587
LifeSpan Biosciences
catalog: LS-C352587

domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot analysis of nm23-H2 expression in MCF7 (A); HeLa (B); A431 (C); Jurkat (D); K562 (E); BJAB (F) whole cell lysates.
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NME2 Antibody (60-140 aa, Internal) LS-C384920
LifeSpan Biosciences
catalog: LS-C384920

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA

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