IHC-plus™ NFYB Antibody (N-Terminus) LS-B718
LifeSpan Biosciences
catalog: LS-B718

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section

Anti-NFYB antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.
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NFYB Antibody (N-Terminus) LS-C9326
LifeSpan Biosciences
catalog: LS-C9326

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunoprecipitation

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NFYB Antibody (N-Terminus) LS-C9329
LifeSpan Biosciences
catalog: LS-C9329

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunoprecipitation

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NFYB Antibody (N-Terminus) LS-C18766
LifeSpan Biosciences
catalog: LS-C18766

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA

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NFYB Antibody (N-Terminus) LS-C30346
LifeSpan Biosciences
catalog: LS-C30346

domestic rabbit polyclonal
reactivity: human
application: western blot

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NFYB Antibody LS-C73809
LifeSpan Biosciences
catalog: LS-C73809

mouse monoclonal
reactivity: human
application: western blot

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NFYB Antibody (C-Terminus) LS-C109581
LifeSpan Biosciences
catalog: LS-C109581

domestic rabbit polyclonal
reactivity: human
application: western blot

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NFYB Antibody (aa1-50) LS-C118837
LifeSpan Biosciences
catalog: LS-C118837

domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot analysis of lysates from 293 cells, using NFYB Antibody. The lane on the right is blocked with the synthesized peptide.
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NFYB Antibody (aa17-46) LS-C164858
LifeSpan Biosciences
catalog: LS-C164858

domestic rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry

NFYB Antibody western blot of A549 cell line lysates (35 ug/lane). The NFYB antibody detected the NFYB protein (arrow).

Fluorescent confocal image of HeLa cell stained with NFYB Antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NFYB primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). NFYB immunoreactivity is localized to nucleus significantly.
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NFYB Antibody (clone NFYBA7) LS-C184290
LifeSpan Biosciences
catalog: LS-C184290

mouse monoclonal (NFYBA7)
reactivity: human
application: western blot, immunocytochemistry

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NFYB Antibody (Gln30) LS-C191956
LifeSpan Biosciences
catalog: LS-C191956

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

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NFYB Antibody (clone 6H6) LS-C197778
LifeSpan Biosciences
catalog: LS-C197778

mouse monoclonal (6H6)
reactivity: human
application: western blot, ELISA

Western Blot analysis of NFYB expression in transfected 293T cell line by NFYB monoclonal antibody (M01), clone 6H6.Lane 1: NFYB transfected lysate(22.8 KDa).Lane 2: Non-transfected lysate.

Detection limit for recombinant GST tagged NFYB is approximately 0.1 ng/ml as a capture antibody.

Western blot analysis of NFYB over-expressed 293 cell line, cotransfected with NFYB Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with NFYB monoclonal antibody (M01), clone 6H6 . GAPDH ( 36.1 kDa ) used as specificity and loading control.
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NFYB Antibody (aa17-46, AP) LS-C264335
LifeSpan Biosciences
catalog: LS-C264335

domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA

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NFYB Antibody (aa17-46, APC) LS-C264336
LifeSpan Biosciences
catalog: LS-C264336

domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA

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NFYB Antibody (aa17-46, Biotin) LS-C264337
LifeSpan Biosciences
catalog: LS-C264337

domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA

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NFYB Antibody (aa17-46, FITC) LS-C264338
LifeSpan Biosciences
catalog: LS-C264338

domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA

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NFYB Antibody (aa17-46, Azide-free, HRP) LS-C264339
LifeSpan Biosciences
catalog: LS-C264339

domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA

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NFYB Antibody (aa17-46, PE) LS-C264340
LifeSpan Biosciences
catalog: LS-C264340

domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA

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NFYB Antibody (aa17-46) LS-C323325
LifeSpan Biosciences
catalog: LS-C323325

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA

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NFYB Antibody LS-C346341
LifeSpan Biosciences
catalog: LS-C346341

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry

Western blot analysis of extracts of various cell lines.

Immunofluorescence analysis of MCF7 cells.
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NFYB Antibody (N-Terminus) LS-C368656
LifeSpan Biosciences
catalog: LS-C368656

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Western blot analysis of NFYB expression in A549 (A); HEK293T (B) whole cell lysates.

Immunohistochemical analysis of NFYB staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected with HRP and DAB as chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
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NFYB Antibody (10-90 aa, N-terminal) LS-C384859
LifeSpan Biosciences
catalog: LS-C384859

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry

Western blot of NF-YB antibody
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