LifeSpan Biosciences IkappaBalpha antibody

NFKBIA / IKB Alpha / IKBA Antibody (phospho-Ser32/Ser36, clone 39A1413) LS-C583
LifeSpan Biosciences
catalog: LS-C583

mouse monoclonal (39A1413)
reactivity: human
modification: phosphorylated
application: western blot, immunoprecipitation

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IHC-plus™ NFKBIA / IKB Alpha / IKBA Antibody (N-Terminus) LS-B1550
LifeSpan Biosciences
catalog: LS-B1550

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Anti-IKB Alpha antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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NFKBIA / IKB Alpha / IKBA Antibody (clone 5D1623) LS-C512
LifeSpan Biosciences
catalog: LS-C512

mouse monoclonal (5D1623)
reactivity: human
application: western blot

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NFKBIA / IKB Alpha / IKBA Antibody (aa32-291, clone 6A920) LS-C552
LifeSpan Biosciences
catalog: LS-C552

mouse monoclonal (6A920)
reactivity: human
application: western blot, immunoprecipitation, flow cytometry

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NFKBIA / IKB Alpha / IKBA Antibody LS-C718
LifeSpan Biosciences
catalog: LS-C718

domestic rabbit polyclonal
reactivity: human
application: western blot

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NFKBIA / IKB Alpha / IKBA Antibody LS-C15880
LifeSpan Biosciences
catalog: LS-C15880

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot

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NFKBIA / IKB Alpha / IKBA Antibody (phospho-Ser32/Ser36) LS-C15881
LifeSpan Biosciences
catalog: LS-C15881

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot

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NFKBIA / IKB Alpha / IKBA Antibody (C-Terminus) LS-C24619
LifeSpan Biosciences
catalog: LS-C24619

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunoprecipitation

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NFKBIA / IKB Alpha / IKBA Antibody LS-C24621
LifeSpan Biosciences
catalog: LS-C24621

domestic sheep polyclonal
reactivity: human
application: western blot, ELISA, immunoprecipitation

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NFKBIA / IKB Alpha / IKBA Antibody LS-C24626
LifeSpan Biosciences
catalog: LS-C24626

mouse monoclonal
reactivity: human
application: western blot

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NFKBIA / IKB Alpha / IKBA Antibody LS-C24630
LifeSpan Biosciences
catalog: LS-C24630

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot

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IHC-plus™ NFKBIA / IKB Alpha / IKBA Antibody (C-Terminus) LS-B717
LifeSpan Biosciences
catalog: LS-B717

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section

Anti-IKB Alpha antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:1000.

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NFKBIA / IKB Alpha / IKBA Antibody LS-C24620
LifeSpan Biosciences
catalog: LS-C24620

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunoprecipitation

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IHC-plus™ NFKBIA / IKB Alpha / IKBA Antibody (C-Terminus) LS-B1640
LifeSpan Biosciences
catalog: LS-B1640

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, immunohistochemistry, immunoprecipitation, immunohistochemistry - paraffin section

Anti-IKB Alpha antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.

Hela cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbit anti-IkappaBalpha polyclonal antibody. Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence system.
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NFKBIA / IKB Alpha / IKBA Antibody LS-C48273
LifeSpan Biosciences
catalog: LS-C48273

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot

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NFKBIA / IKB Alpha / IKBA Antibody LS-C48310
LifeSpan Biosciences
catalog: LS-C48310

mouse monoclonal
reactivity: human
application: western blot, immunohistochemistry, immunoprecipitation

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NFKBIA / IKB Alpha / IKBA Antibody LS-C85864
LifeSpan Biosciences
catalog: LS-C85864

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunoprecipitation

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NFKBIA / IKB Alpha / IKBA Antibody (aa1-30) LS-C98467
LifeSpan Biosciences
catalog: LS-C98467

domestic rabbit polyclonal
reactivity: human
application: western blot

The anti-IKB-alpha N-term antibody is used in Western blot to detect IKB-alpha in Jurkat cell lysate.
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NFKBIA / IKB Alpha / IKBA Antibody (N-Terminus) LS-C112275
LifeSpan Biosciences
catalog: LS-C112275

domestic rabbit polyclonal
reactivity: human
application: western blot

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NFKBIA / IKB Alpha / IKBA Antibody (C-Terminus) LS-C112311
LifeSpan Biosciences
catalog: LS-C112311

domestic rabbit polyclonal
reactivity: human
application: western blot

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NFKBIA / IKB Alpha / IKBA Antibody (phospho-Tyr42) LS-C117383
LifeSpan Biosciences
catalog: LS-C117383

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: immunohistochemistry, immunohistochemistry - paraffin section

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using IkappaB-alpha (Phospho-Tyr42) Antibody. The picture on the right is blocked with the phospho peptide.
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NFKBIA / IKB Alpha / IKBA Antibody (phospho-Ser32/Ser36) LS-C123815
LifeSpan Biosciences
catalog: LS-C123815

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: immunohistochemistry

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NFKBIA / IKB Alpha / IKBA Antibody (phospho-Tyr42) LS-C123816
LifeSpan Biosciences
catalog: LS-C123816

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: immunohistochemistry

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NFKBIA / IKB Alpha / IKBA Antibody LS-C123817
LifeSpan Biosciences
catalog: LS-C123817

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

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NFKBIA / IKB Alpha / IKBA Antibody LS-C123818
LifeSpan Biosciences
catalog: LS-C123818

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

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NFKBIA / IKB Alpha / IKBA Antibody (C-Terminus) LS-C124263
LifeSpan Biosciences
catalog: LS-C124263

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

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IHC-plus™ NFKBIA / IKB Alpha / IKBA Antibody (aa34-48) LS-B6500
LifeSpan Biosciences
catalog: LS-B6500

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Anti-IKB Alpha antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
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NFKBIA / IKB Alpha / IKBA Antibody (aa130-143) LS-C147914
LifeSpan Biosciences
catalog: LS-C147914

domestic rabbit polyclonal
reactivity: human
application: ELISA

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NFKBIA / IKB Alpha / IKBA Antibody (aa32-291, clone 6A920, Biotin) LS-C147975
LifeSpan Biosciences
catalog: LS-C147975

mouse monoclonal (6A920)
reactivity: human
conjugate: biotin
application: ELISA

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NFKBIA / IKB Alpha / IKBA Antibody (phospho-Ser32/Ser36, clone 39A1413, Biotin) ...
LifeSpan Biosciences
catalog: LS-C147980

mouse monoclonal (39A1413)
reactivity: human
conjugate: biotin
modification: phosphorylated
application: ELISA

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NFKBIA / IKB Alpha / IKBA Antibody (phospho-Ser32) LS-C163651
LifeSpan Biosciences
catalog: LS-C163651

domestic rabbit polyclonal
reactivity: human

Dot blot of anti-Phospho-NFKBIA (Ser32) Antibody Phospho-specific antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
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NFKBIA / IKB Alpha / IKBA Antibody (aa12-41) LS-C164850
LifeSpan Biosciences
catalog: LS-C164850

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section

Western blot of NFKBIA-S32/36 in HeLa cell line lysates (35 ug/lane). NFKBIA (arrow) was detected using the purified antibody.

Formalin-fixed and paraffin-embedded human hepatocarcinoma with NFKBIA Antibody (S32/36 ), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Flow cytometric of HeLa cells using NFKBIA Antibody ( S32/36 )(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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NFKBIA / IKB Alpha / IKBA Antibody (N-Terminus) LS-C169491
LifeSpan Biosciences
catalog: LS-C169491

domestic rabbit polyclonal
reactivity: human
application: western blot

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NFKBIA / IKB Alpha / IKBA Antibody (Ser36) LS-C177650
LifeSpan Biosciences
catalog: LS-C177650

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot of IB- (S36) pAb in extracts from 293 cells.

IHC of IB- (S36) pAb in paraffin-embedded human breast carcinoma tissue.
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NFKBIA / IKB Alpha / IKBA Antibody (phospho-Tyr42) LS-C178012
LifeSpan Biosciences
catalog: LS-C178012

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: immunohistochemistry, immunohistochemistry - paraffin section

IHC of p-IB- (Y42) pAb in paraffin-embedded human breast carcinoma tissue.
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NFKBIA / IKB Alpha / IKBA Antibody (N-Terminus) LS-C178359
LifeSpan Biosciences
catalog: LS-C178359

mouse monoclonal
reactivity: human
application: western blot

NFKBIA(N-terminus) antibody at 1/1000 dilution Lane1:Jurkat cell lysate 40 ug/Lane Lane2: HeLa cell lysate 40 ug/Lane.
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NFKBIA / IKB Alpha / IKBA Antibody (clone IKB5I328) LS-C184252
LifeSpan Biosciences
catalog: LS-C184252

mouse monoclonal (IKB5I328)
reactivity: human
application: ELISA

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NFKBIA / IKB Alpha / IKBA Antibody (aa1-317) LS-C186162
LifeSpan Biosciences
catalog: LS-C186162

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

IKB alpha antibody detects IKB alpha protein by Western blot analysis. A. 30 ug BCL-1 whole cell lysate/extract. B. 30 ug C2C12 whole cell lysate/extract. 10 % SDS-PAGE. IKB alpha antibody dilution:1:5000

Immunofluorescence of paraformaldehyde-fixed HeLa using IkappaB-alpha antibody at 1:200 dilution.

IKB alpha antibody detects NFKBIA protein at nucleus on U251 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded U251 xenograft. IKB alpha antibody dilution:1:500.
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NFKBIA / IKB Alpha / IKBA Antibody (clone 3H4) LS-C197775
LifeSpan Biosciences
catalog: LS-C197775

mouse monoclonal (3H4)
reactivity: human
application: western blot, ELISA

Western Blot analysis of NFKBIA expression in transfected 293T cell line by NFKBIA monoclonal antibody (M10), clone 3H4.Lane 1: NFKBIA transfected lysate (Predicted MW: 35.6 KDa).Lane 2: Non-transfected lysate.

Detection limit for recombinant GST tagged NFKBIA is 0.03 ng/ml as a capture antibody.
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NFKBIA / IKB Alpha / IKBA Antibody (phospho-Ser32/Ser36) LS-C199097
LifeSpan Biosciences
catalog: LS-C199097

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Immunofluorescence analysis of LOVO cells, using IkappaB-alpha (Phospho-Ser32/Ser36) Antibody. The picture on the right is blocked with the phospho peptide.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using IkappaB-alpha (Phospho-Ser32/Ser36) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from COS7 cells, using IkappaB-alpha (Phospho-Ser32/Ser36) Antibody. The lane on the right is blocked with the phospho peptide.
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NFKBIA / IKB Alpha / IKBA Antibody (phospho-Tyr305) LS-C199098
LifeSpan Biosciences
catalog: LS-C199098

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Immunohistochemistry analysis of paraffin-embedded human lymph node, using IkappaB-alpha (Phospho-Tyr305) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from COS7 cells treated with nocodazole 1ug/ml 16h, using IkappaB-alpha (Phospho-Tyr305) Antibody. The lane on the right is blocked with the phospho peptide.
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NFKBIA / IKB Alpha / IKBA Antibody (clone 1121CT8.6.1) LS-C204023
LifeSpan Biosciences
catalog: LS-C204023

mouse monoclonal (1121CT8.6.1)
reactivity: human
application: western blot

NFKBIA Antibody western blot of HeLa,mouse NIH/3T3 cell line lysates (35 ug/lane). The NFKBIA antibody detected the NFKBIA protein (arrow).
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NFKBIA / IKB Alpha / IKBA Antibody (phospho-Ser32, AP) LS-C264312
LifeSpan Biosciences
catalog: LS-C264312

domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: ELISA

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NFKBIA / IKB Alpha / IKBA Antibody (phospho-Ser32, APC) LS-C264314
LifeSpan Biosciences
catalog: LS-C264314

domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: ELISA

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NFKBIA / IKB Alpha / IKBA Antibody (phospho-Ser32, FITC) LS-C264317
LifeSpan Biosciences
catalog: LS-C264317

domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: ELISA

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NFKBIA / IKB Alpha / IKBA Antibody (phospho-Ser32, Azide-free, HRP) LS-C264318
LifeSpan Biosciences
catalog: LS-C264318

domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: ELISA

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NFKBIA / IKB Alpha / IKBA Antibody (phospho-Ser32, PE) LS-C264319
LifeSpan Biosciences
catalog: LS-C264319

domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: ELISA

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NFKBIA / IKB Alpha / IKBA Antibody (aa1-30, AP) LS-C280087
LifeSpan Biosciences
catalog: LS-C280087

domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA

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NFKBIA / IKB Alpha / IKBA Antibody (aa1-30, APC) LS-C280088
LifeSpan Biosciences
catalog: LS-C280088

domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA

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NFKBIA / IKB Alpha / IKBA Antibody (aa1-30, Biotin) LS-C280091
LifeSpan Biosciences
catalog: LS-C280091

domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA

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NFKBIA / IKB Alpha / IKBA Antibody (aa1-30, FITC) LS-C280093
LifeSpan Biosciences
catalog: LS-C280093

domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA

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NFKBIA / IKB Alpha / IKBA Antibody (aa1-30, PE) LS-C280094
LifeSpan Biosciences
catalog: LS-C280094

domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA

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NFKBIA / IKB Alpha / IKBA Antibody (aa1-30, Azide-free, HRP) LS-C280095
LifeSpan Biosciences
catalog: LS-C280095

domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA

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NFKBIA / IKB Alpha / IKBA Antibody (aa12-41, AP) LS-C280096
LifeSpan Biosciences
catalog: LS-C280096

domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA

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NFKBIA / IKB Alpha / IKBA Antibody (aa12-41, APC) LS-C280097
LifeSpan Biosciences
catalog: LS-C280097

domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA

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NFKBIA / IKB Alpha / IKBA Antibody (aa12-41, Biotin) LS-C280098
LifeSpan Biosciences
catalog: LS-C280098

domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA

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NFKBIA / IKB Alpha / IKBA Antibody (aa12-41, FITC) LS-C280099
LifeSpan Biosciences
catalog: LS-C280099

domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA

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NFKBIA / IKB Alpha / IKBA Antibody (aa12-41, Azide-free, HRP) LS-C280100
LifeSpan Biosciences
catalog: LS-C280100

domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA

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NFKBIA / IKB Alpha / IKBA Antibody (aa12-41, PE) LS-C280103
LifeSpan Biosciences
catalog: LS-C280103

domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA

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NFKBIA / IKB Alpha / IKBA Antibody (aa268-317) LS-C286251
LifeSpan Biosciences
catalog: LS-C286251

domestic rabbit polyclonal
reactivity: human
application: immunohistochemistry, immunocytochemistry

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NFKBIA / IKB Alpha / IKBA Antibody (aa35-84) LS-C286287
LifeSpan Biosciences
catalog: LS-C286287

domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot analysis of the lysates from HeLa cells using IkappaB-alpha antibody.
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NFKBIA / IKB Alpha / IKBA Antibody (aa74-247) LS-C294629
LifeSpan Biosciences
catalog: LS-C294629

domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot of recombinant NFKBIA / IKB Alpha / IKBA. This image was taken for the unconjugated form of this product. Other forms have not been tested.

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NFKBIA / IKB Alpha / IKBA Antibody (aa74-247, Biotin) LS-C299577
LifeSpan Biosciences
catalog: LS-C299577

domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot

Western blot of recombinant NFKBIA / IKB Alpha / IKBA. This image was taken for the unconjugated form of this product. Other forms have not been tested.

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NFKBIA / IKB Alpha / IKBA Antibody LS-C192447
LifeSpan Biosciences
catalog: LS-C192447

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

Western blot of NFKBIA pAb in extracts from HL60 cells.
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NFKBIA / IKB Alpha / IKBA Antibody (aa1-30) LS-C328023
LifeSpan Biosciences
catalog: LS-C328023

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA

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NFKBIA / IKB Alpha / IKBA Antibody (aa12-41) LS-C328029
LifeSpan Biosciences
catalog: LS-C328029

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA

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NFKBIA / IKB Alpha / IKBA Antibody (phospho-Ser32) LS-C329897
LifeSpan Biosciences
catalog: LS-C329897

domestic rabbit polyclonal
reactivity: human
application: ELISA

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NFKBIA / IKB Alpha / IKBA Antibody LS-C331335
LifeSpan Biosciences
catalog: LS-C331335

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

Immunohistochemistry of paraffin-embedded mouse kidney tissue.

Immunofluorescence analysis of U2OS cells.

Western blot analysis of extracts of various cell lines, using NFKBIA antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 3s.
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NFKBIA / IKB Alpha / IKBA Antibody (phospho-Tyr42) LS-C336061
LifeSpan Biosciences
catalog: LS-C336061

domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot analysis of extracts of HeLa cellss.
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NFKBIA / IKB Alpha / IKBA Antibody (clone OTI1D4) LS-C338406
LifeSpan Biosciences
catalog: LS-C338406

mouse monoclonal (OTI1D4)
reactivity: human
application: western blot, immunocytochemistry

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NFKBIA (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NFKBIA.

Immunofluorescent staining of HeLa cells using anti-NFKBIA mouse monoclonal antibody.
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NFKBIA / IKB Alpha / IKBA Antibody (clone OTI2F5) LS-C338411
LifeSpan Biosciences
catalog: LS-C338411

mouse monoclonal (OTI2F5)
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

IHC of paraffin-embedded Human colon tissue using anti-NFKBIA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min).
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NFKBIA / IKB Alpha / IKBA Antibody (phospho-Ser32/Ser36) LS-C352580
LifeSpan Biosciences
catalog: LS-C352580

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot analysis of IKB alpha (pS32/S36) expression in HepG2 TNFa-treated (A); Raw264.7 TNFa-treated (B); PC12 TNFa-treated (C) whole cell lysates.

Immunohistochemical analysis of IKB alpha (pS32/S36) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Immunofluorescent analysis of IKB alpha (pS32/S36) staining in Raw264.7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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NFKBIA / IKB Alpha / IKBA Antibody (N-Terminus) LS-C352583
LifeSpan Biosciences
catalog: LS-C352583

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

Western blot analysis of IKB alpha expression in THP1 (A); HeLa (B); Raw264.7 (C) whole cell lysates.

Immunohistochemical analysis of IKB alpha staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Immunofluorescent analysis of IKB alpha staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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NFKBIA / IKB Alpha / IKBA Antibody (N-Terminus) LS-C352590
LifeSpan Biosciences
catalog: LS-C352590

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot analysis of IKB alpha expression in HeLa (A); HepG2 (B); Raw264.7 (C) whole cell lysates.

Immunofluorescent analysis of IKB alpha staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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IHC-plus™ NFKBIA / IKB Alpha / IKBA Antibody (aa9-58) LS-B12430
LifeSpan Biosciences
catalog: LS-B12430

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)

Western blot analysis of lysates from HUVEC cells, treated with TNF-alpha 20ng/ml 30', using IkappaB-alpha Antibody. The lane on the right is blocked with the synthesized peptide.
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IHC-plus™ NFKBIA / IKB Alpha / IKBA Antibody (aa15-64) LS-B12432
LifeSpan Biosciences
catalog: LS-B12432

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)

Western blot analysis of lysates from MCF7 cells, treated with TNF-alpha, using IkappaB-alpha Antibody. The lane on the right is blocked with the synthesized peptide.
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NFKBIA / IKB Alpha / IKBA Antibody (phospho-Tyr305) LS-C357190
LifeSpan Biosciences
catalog: LS-C357190

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Western blot of extracts from COS7 cells, treated with nocodazole (1 ug/ml, 16hours), using I kappa B- alpha (Phospho-Tyr305) antibody.

Immunohistochemistry of paraffin-embedded human lymph node tissue using I kappa B- alpha (Phospho-Tyr305) antibody.
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NFKBIA / IKB Alpha / IKBA Antibody (aa3-112) LS-C357485
LifeSpan Biosciences
catalog: LS-C357485

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

IKB alpha antibody Western blot. All lanes: Anti IKB alpha at 0.5 ug/ml. Lane 1: Rat Brain Tissue Lysate at 50 ug. Lane 2: Mouse Brain Tissue Lysate at 50 ug. Lane 3: Rat Kidney Tissue Lysate at 50 ug. Lane 4: Mouse Kidney Tissue Lysate at 50 ug. Lane 5: 293T Whole Cell Lysate at 40 ug. Lane 6: JURKAT Whole Cell Lysate at 40 ug. Lane 7: RAJI Whole Cell Lysate at 40 ug. Predicted band size: 36 kD. Observed band size: 36 kD.

IKB alpha antibody IHC-paraffin: Mouse Intestine Tissue.
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NFKBIA / IKB Alpha / IKBA Antibody (aa32-36, clone 39A1413, Azide-free) LS-C3683 ...
LifeSpan Biosciences
catalog: LS-C368314

mouse monoclonal (39A1413)
reactivity: human
application: western blot, immunoprecipitation

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NFKBIA / IKB Alpha / IKBA Antibody (10-90 aa) LS-C380764
LifeSpan Biosciences
catalog: LS-C380764

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, ELISA, immunohistochemistry, immunocytochemistry

Western blot of Phospho-IkappaB-alpha (S32/S36) antibody
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NFKBIA / IKB Alpha / IKBA Antibody (20-100 aa) LS-C380768
LifeSpan Biosciences
catalog: LS-C380768

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, ELISA, immunohistochemistry

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NFKBIA / IKB Alpha / IKBA Antibody (240-320 aa) LS-C381364
LifeSpan Biosciences
catalog: LS-C381364

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, ELISA, immunohistochemistry

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NFKBIA / IKB Alpha / IKBA Antibody (10-90 aa, N-terminal) LS-C384224
LifeSpan Biosciences
catalog: LS-C384224

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA

Western blot of IkappaB-alpha antibody
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NFKBIA / IKB Alpha / IKBA Antibody (240-320 aa) LS-C384227
LifeSpan Biosciences
catalog: LS-C384227

domestic rabbit polyclonal
reactivity: human
application: ELISA, immunohistochemistry, immunocytochemistry

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NFKBIA / IKB Alpha / IKBA Antibody (20-100 aa) LS-C384228
LifeSpan Biosciences
catalog: LS-C384228

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry

Western blot of IkappaB-alpha antibody
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NFKBIA / IKB Alpha / IKBA Antibody (10-90 aa) LS-C384234
LifeSpan Biosciences
catalog: LS-C384234

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry

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NFKBIA / IKB Alpha / IKBA Antibody (Internal) LS-C387830
LifeSpan Biosciences
catalog: LS-C387830

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

Immunohistochemistry of IkappaB-alpha antibody

Western blot of IkappaB-alpha antibody
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price:
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