NFAT4 / NFATC3 Antibody LS-C73517
LifeSpan Biosciences
catalog: LS-C73517

mouse monoclonal
reactivity: human
application: western blot

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NFAT4 / NFATC3 Antibody (aa131-180) LS-C117674
LifeSpan Biosciences
catalog: LS-C117674

domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot analysis of lysates from HeLa cells, treated with Ca+ 40nM 30', using NFAT4 Antibody. The lane on the right is blocked with the synthesized peptide.
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IHC-plus™ NFAT4 / NFATC3 Antibody (aa324-373) LS-B6971
LifeSpan Biosciences
catalog: LS-B6971

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Anti-NFATC3 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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NFAT4 / NFATC3 Antibody (Glu159) LS-C176434
LifeSpan Biosciences
catalog: LS-C176434

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

Western blot of NFAT4 (E159) pAb in extracts from HeLa cells treated with Ca+ 40nM 30'.
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NFAT4 / NFATC3 Antibody (clone 442C2a) LS-C184532
LifeSpan Biosciences
catalog: LS-C184532

mouse monoclonal (442C2a)
reactivity: human

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NFAT4 / NFATC3 Antibody (phospho-Ser165) LS-C199312
LifeSpan Biosciences
catalog: LS-C199312

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunocytochemistry

Immunofluorescence analysis of HeLa cells, using NFAT4 (Phospho-Ser165) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from HeLa cells treated with Ca+ 40nM 30', using NFAT4 (Phospho-Ser165) Antibody. The lane on the right is blocked with the phospho peptide.
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NFAT4 / NFATC3 Antibody (aa100-150) LS-C290237
LifeSpan Biosciences
catalog: LS-C290237

domestic rabbit polyclonal
reactivity: human
application: western blot, immunoprecipitation

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NFAT4 / NFATC3 Antibody (aa700-750) LS-C290238
LifeSpan Biosciences
catalog: LS-C290238

domestic rabbit polyclonal
reactivity: human
application: western blot, immunoprecipitation

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NFAT4 / NFATC3 Antibody (aa1025-1075) LS-C290239
LifeSpan Biosciences
catalog: LS-C290239

domestic rabbit polyclonal
reactivity: human
application: western blot, immunoprecipitation

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NFAT4 / NFATC3 Antibody LS-C334862
LifeSpan Biosciences
catalog: LS-C334862

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry

Western blot analysis of extracts of various cell lines.

Immunofluorescence analysis of HeLa cells.
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NFAT4 / NFATC3 Antibody (N-Terminus) LS-C345231
LifeSpan Biosciences
catalog: LS-C345231

domestic rabbit polyclonal
reactivity: human
application: western blot

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NFAT4 / NFATC3 Antibody (phospho-Ser165) LS-C356217
LifeSpan Biosciences
catalog: LS-C356217

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot analysis of NFAT4 (pS165) expression in Ramos (A); HeLa (B) whole cell lysates.

Immunohistochemical analysis of NFAT4 (pS165) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Immunofluorescent analysis of NFAT4 (pS165) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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IHC-plus™ NFAT4 / NFATC3 Antibody (clone 3A12) LS-B12934
LifeSpan Biosciences
catalog: LS-B12934

mouse monoclonal (3A12)
reactivity: human
application: ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Immunofluorescence of monoclonal antibody to NFATC3 on HeLa cell. [antibody concentration 10 ug/ml].

Detection limit for recombinant GST tagged NFATC3 is approximately 10 ng/ml as a capture antibody.

Human Thymus: Formalin-Fixed, Paraffin-Embedded (FFPE)
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NFAT4 / NFATC3 Antibody (100-180 aa) LS-C381121
LifeSpan Biosciences
catalog: LS-C381121

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, ELISA, immunohistochemistry, immunocytochemistry

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NFAT4 / NFATC3 Antibody (100-180 aa) LS-C384850
LifeSpan Biosciences
catalog: LS-C384850

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry

Western blot of NFATc3 antibody
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