IHC-plus™ NFIA / Nuclear Factor 1 Antibody (aa348-397) LS-B1549
LifeSpan Biosciences
catalog: LS-B1549
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Anti-NFIA antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

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NFIA / Nuclear Factor 1 Antibody LS-C73763
LifeSpan Biosciences
catalog: LS-C73763
mouse monoclonal
reactivity: human
application: western blot
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NFIA / Nuclear Factor 1 Antibody (C-Terminus) LS-C135063
LifeSpan Biosciences
catalog: LS-C135063
domestic rabbit polyclonal
reactivity: human, mouse
application: western blot
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NFIA / Nuclear Factor 1 Antibody (aa201-250) LS-C170121
LifeSpan Biosciences
catalog: LS-C170121
domestic rabbit polyclonal
reactivity: human, mouse
application: western blot
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NFIA / Nuclear Factor 1 Antibody (clone 438C1a) LS-C184646
LifeSpan Biosciences
catalog: LS-C184646
mouse monoclonal (438C1a)
reactivity: human
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IHC-plus™ NFIA / Nuclear Factor 1 Antibody (aa470-498) LS-B9077
LifeSpan Biosciences
catalog: LS-B9077
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Anti-NFIA / Nuclear Factor 1 antibody IHC staining of human skin, epidermis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

NFIA Antibody western blot of HepG2 cell line lysates (35 ug/lane). The NFIA antibody detected the NFIA protein (arrow).

Fluorescent confocal image of HeLa cell stained with NFIA Antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NFIA primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). NFIA immunoreactivity is localized to Nucleus significantly.
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NFIA / Nuclear Factor 1 Antibody (aa470-498, APC) LS-C246594
LifeSpan Biosciences
catalog: LS-C246594
domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA
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NFIA / Nuclear Factor 1 Antibody (aa470-498, AP) LS-C246595
LifeSpan Biosciences
catalog: LS-C246595
domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA
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NFIA / Nuclear Factor 1 Antibody (aa470-498, Biotin) LS-C246596
LifeSpan Biosciences
catalog: LS-C246596
domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA
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NFIA / Nuclear Factor 1 Antibody (aa470-498, FITC) LS-C246597
LifeSpan Biosciences
catalog: LS-C246597
domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA
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NFIA / Nuclear Factor 1 Antibody (aa470-498, PE) LS-C246598
LifeSpan Biosciences
catalog: LS-C246598
domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA
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NFIA / Nuclear Factor 1 Antibody (aa470-498, Azide-free, HRP) LS-C248907
LifeSpan Biosciences
catalog: LS-C248907
domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA
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NFIA / Nuclear Factor 1 Antibody (aa470-498) LS-C323312
LifeSpan Biosciences
catalog: LS-C323312
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA
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NFIA / Nuclear Factor 1 Antibody LS-C332448
LifeSpan Biosciences
catalog: LS-C332448
domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot analysis of extracts of mouse brain cells.
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NFIA / Nuclear Factor 1 Antibody (aa384-418) LS-C344561
LifeSpan Biosciences
catalog: LS-C344561
domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot of lysates from HeLa cell line, rat liver tissue lysate (from left to right) with NFIA Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20 ug per lane.
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NFIA / Nuclear Factor 1 Antibody (N-Terminus) LS-C354251
LifeSpan Biosciences
catalog: LS-C354251
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Western blot analysis of NFIA expression in Jurkat (A); Raw264.7 (B); rat brain (C) whole cell lysates.

Immunohistochemical analysis of NFIA staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
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NFIA / Nuclear Factor 1 Antibody (aa1-143) LS-C374625
LifeSpan Biosciences
catalog: LS-C374625
domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot of NFIA / Nuclear Factor 1 antibody.

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NFIA / Nuclear Factor 1 Antibody (30-110 aa, N-terminal) LS-C384844
LifeSpan Biosciences
catalog: LS-C384844
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry
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