IHC-plus™ Neurofibromin / NF1 Antibody (aa27-41, clone McNFn27) LS-B217
LifeSpan Biosciences
catalog: LS-B217

mouse monoclonal (McNFn27)
reactivity: human
application: ELISA, immunohistochemistry, immunoprecipitation, immunohistochemistry - paraffin section

Anti-NF1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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Neurofibromin / NF1 Antibody (aa27-41, clone McNFn27a) LS-C2721
LifeSpan Biosciences
catalog: LS-C2721

mouse monoclonal (McNFn27a)
reactivity: human
application: western blot, ELISA, immunoprecipitation

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Neurofibromin / NF1 Antibody (N-Terminus, clone McNFn27a, Biotin) LS-C142055
LifeSpan Biosciences
catalog: LS-C142055

mouse monoclonal (McNFn27a)
reactivity: human
conjugate: biotin
application: western blot, ELISA, immunoprecipitation

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Neurofibromin / NF1 Antibody (N-Terminus, clone McNFn27a, DY650) LS-C142056
LifeSpan Biosciences
catalog: LS-C142056

mouse monoclonal (McNFn27a)
reactivity: human
application: western blot, ELISA, immunoprecipitation

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Neurofibromin / NF1 Antibody (N-Terminus, clone McNFn27a, DY488) LS-C142057
LifeSpan Biosciences
catalog: LS-C142057

mouse monoclonal (McNFn27a)
reactivity: human
application: western blot, ELISA, immunoprecipitation

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Neurofibromin / NF1 Antibody (N-Terminus, clone McNFn27a, DY550) LS-C142059
LifeSpan Biosciences
catalog: LS-C142059

mouse monoclonal (McNFn27a)
reactivity: human
application: western blot, ELISA, immunoprecipitation

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IHC-plus™ Neurofibromin / NF1 Antibody (aa1551-1600) LS-B6339
LifeSpan Biosciences
catalog: LS-B6339

domestic rabbit polyclonal
reactivity: human
application: immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Anti-NF1 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.

Immunofluorescence analysis of HepG2 cells, using NF1 Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from HepG2 cells, using NF1 Antibody. The lane on the right is blocked with the synthesized peptide.
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IHC-plus™ Neurofibromin / NF1 Antibody (aa27-41) LS-B8110
LifeSpan Biosciences
catalog: LS-B8110

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

Anti-Neurofibromin / NF1 antibody IHC of human brain, cortex neurons. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
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Neurofibromin / NF1 Antibody (clone 2D1) LS-C197771
LifeSpan Biosciences
catalog: LS-C197771

mouse monoclonal (2D1)
reactivity: human
application: western blot, ELISA

Detection limit for recombinant GST tagged NF1 is approximately 3 ng/ml as a capture antibody.
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Neurofibromin / NF1 Antibody (Internal) LS-C352564
LifeSpan Biosciences
catalog: LS-C352564

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot analysis of NF1 expression in A431 (A); HeLa (B) whole cell lysates.

Immunohistochemical analysis of NF1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Immunofluorescent analysis of NF1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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Neurofibromin / NF1 Antibody (1520-1600 aa, Internal) LS-C384834
LifeSpan Biosciences
catalog: LS-C384834

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunocytochemistry

Western blot of Neurofibromin antibody
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