IHC-plus™ NEK2 Antibody (clone 2F6) LS-B5478
LifeSpan Biosciences
catalog: LS-B5478
mouse monoclonal (2F6)
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunoprecipitation, immunohistochemistry - paraffin section

Western blot of NEK2 expression in transfected 293T cell line by NEK2 monoclonal antibody clone 2F6.

Detection limit for recombinant GST tagged NEK2 is approximately 0.3 ng/ml as a capture antibody.

Anti-NEK2 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
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IHC-plus™ NEK2 Antibody LS-B129
LifeSpan Biosciences
catalog: LS-B129
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
citations: 3

Anti-NEK2 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.

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NEK2 Antibody (aa287-299) LS-C9228
LifeSpan Biosciences
catalog: LS-C9228
domestic rabbit polyclonal
reactivity: human
application: ELISA
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NEK2 Antibody (aa282-295) LS-C82239
LifeSpan Biosciences
catalog: LS-C82239
domestic sheep polyclonal
reactivity: human
application: western blot

Western blot of NEK2 antibody.
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NEK2 Antibody (aa350-382) LS-C101165
LifeSpan Biosciences
catalog: LS-C101165
domestic rabbit polyclonal
reactivity: human
application: western blot

The anti-NEK2 antibody is used in Western blot to detect NEK2 in HeLa cell lysate.
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NEK2 Antibody (aa350-382, AP) LS-C280024
LifeSpan Biosciences
catalog: LS-C280024
domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA
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NEK2 Antibody (aa350-382, Biotin) LS-C280025
LifeSpan Biosciences
catalog: LS-C280025
domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA
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NEK2 Antibody (aa350-382, APC) LS-C280026
LifeSpan Biosciences
catalog: LS-C280026
domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA
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NEK2 Antibody (aa350-382, FITC) LS-C280027
LifeSpan Biosciences
catalog: LS-C280027
domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA
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NEK2 Antibody (aa350-382, Azide-free, HRP) LS-C280028
LifeSpan Biosciences
catalog: LS-C280028
domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA
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NEK2 Antibody (aa350-382, PE) LS-C280029
LifeSpan Biosciences
catalog: LS-C280029
domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA
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NEK2 Antibody (aa396-426, AP) LS-C280030
LifeSpan Biosciences
catalog: LS-C280030
domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA
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NEK2 Antibody (aa396-426, APC) LS-C280031
LifeSpan Biosciences
catalog: LS-C280031
domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA
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NEK2 Antibody (aa396-426, Biotin) LS-C280032
LifeSpan Biosciences
catalog: LS-C280032
domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA
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NEK2 Antibody (aa396-426, FITC) LS-C280033
LifeSpan Biosciences
catalog: LS-C280033
domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA
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NEK2 Antibody (aa396-426, PE) LS-C280034
LifeSpan Biosciences
catalog: LS-C280034
domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA
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NEK2 Antibody (aa396-426, Azide-free, HRP) LS-C280035
LifeSpan Biosciences
catalog: LS-C280035
domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA
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NEK2 Antibody (aa793-824, AP) LS-C280041
LifeSpan Biosciences
catalog: LS-C280041
domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA
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NEK2 Antibody (aa793-824, APC) LS-C280043
LifeSpan Biosciences
catalog: LS-C280043
domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA
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NEK2 Antibody (aa793-824, Biotin) LS-C280044
LifeSpan Biosciences
catalog: LS-C280044
domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA
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NEK2 Antibody (aa793-824, FITC) LS-C280045
LifeSpan Biosciences
catalog: LS-C280045
domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA
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NEK2 Antibody (aa793-824, PE) LS-C280046
LifeSpan Biosciences
catalog: LS-C280046
domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA
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NEK2 Antibody (aa793-824, Azide-free, HRP) LS-C280047
LifeSpan Biosciences
catalog: LS-C280047
domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA
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NEK2 Antibody (aa1165-1196, Biotin) LS-C280048
LifeSpan Biosciences
catalog: LS-C280048
domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA, immunohistochemistry
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NEK2 Antibody (aa1165-1196, AP) LS-C280049
LifeSpan Biosciences
catalog: LS-C280049
domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA, immunohistochemistry
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NEK2 Antibody (aa1165-1196, FITC) LS-C280050
LifeSpan Biosciences
catalog: LS-C280050
domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA, immunohistochemistry
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NEK2 Antibody (aa1165-1196, APC) LS-C280051
LifeSpan Biosciences
catalog: LS-C280051
domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA, immunohistochemistry
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NEK2 Antibody (aa1165-1196, Azide-free, HRP) LS-C280052
LifeSpan Biosciences
catalog: LS-C280052
domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA, immunohistochemistry
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NEK2 Antibody (aa1165-1196, PE) LS-C280053
LifeSpan Biosciences
catalog: LS-C280053
domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA, immunohistochemistry
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NEK2 Antibody (aa137-400) LS-C295623
LifeSpan Biosciences
catalog: LS-C295623
domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot of recombinant NEK2. This image was taken for the unconjugated form of this product. Other forms have not been tested.

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NEK2 Antibody (aa148-397) LS-C295625
LifeSpan Biosciences
catalog: LS-C295625
domestic rabbit polyclonal
reactivity: human, mouse
application: western blot

Western Blot; Sample: Mouse Testis lysate; Primary Ab: 3µg/ml Rabbit Anti-Mouse NEK2 Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody

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NEK2 Antibody (aa1165-1196) LS-C328011
LifeSpan Biosciences
catalog: LS-C328011
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry
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NEK2 Antibody LS-C334005
LifeSpan Biosciences
catalog: LS-C334005
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry

Western blot analysis of extracts of various cell lines, using NEK2 antibody.

Immunohistochemistry of paraffin-embedded human kidney cancer using NEK2 antibody at dilution of 1:200 (400x lens).

Immunofluorescence analysis of U2OS cell using NEK2 antibody. Blue: DAPI for nuclear staining.
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IHC-plus™ NEK2 Antibody (aa396-426) LS-B11803
LifeSpan Biosciences
catalog: LS-B11803
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE)

Fluorescent image of U251 cells stained with hNEK2-C410. Antibody was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).

Western blot of lysates from 293T, HeLa, Jurkat, K562, MCF-7, and mouse NIH/3T3 cell line (from left to right), using NEK2 Antibody (C410). Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
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NEK2 Antibody LS-C341588
LifeSpan Biosciences
catalog: LS-C341588
domestic rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry
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IHC-plus™ NEK2 Antibody (aa130-179) LS-B11940
LifeSpan Biosciences
catalog: LS-B11940
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Human Heart: Formalin-Fixed, Paraffin-Embedded (FFPE). This image was taken for the unconjugated form of this product. Other forms have not been tested.

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NEK2 Antibody (N-Terminus) LS-C345101
LifeSpan Biosciences
catalog: LS-C345101
domestic rabbit polyclonal
reactivity: human
application: western blot
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NEK2 Antibody (C-Terminus) LS-C345686
LifeSpan Biosciences
catalog: LS-C345686
domestic rabbit polyclonal
reactivity: human
application: western blot
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NEK2 Antibody (aa1-135) LS-C376227
LifeSpan Biosciences
catalog: LS-C376227
domestic rabbit polyclonal
reactivity: human
application: ELISA, immunohistochemistry, immunohistochemistry - paraffin section

Immunohistochemistry of paraffin-embedded human kidney using antibody at 1:100 dilution.
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