LifeSpan Biosciences neurofilament L antibody

PathPlus™ NF-L / NEFL Antibody LS-B257
LifeSpan Biosciences
catalog: LS-B257

domestic rabbit polyclonal
reactivity: human
application: immunohistochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

Anti-NEFL / NF-L antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
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NEFL + NEFH Antibody LS-C131018
LifeSpan Biosciences
catalog: LS-C131018

mouse monoclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

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NF-L / NEFL Antibody (clone DA2) LS-C2725
LifeSpan Biosciences
catalog: LS-C2725

mouse monoclonal (DA2)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

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NEFL + NEFH Antibody LS-C73357
LifeSpan Biosciences
catalog: LS-C73357

mouse monoclonal
reactivity: human
application: western blot, immunohistochemistry

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NF-L / NEFL Antibody (clone DA2) LS-C16795
LifeSpan Biosciences
catalog: LS-C16795

mouse monoclonal (DA2)
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

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NF-L / NEFL Antibody LS-C61819
LifeSpan Biosciences
catalog: LS-C61819

mouse monoclonal
reactivity: human
application: western blot, immunocytochemistry

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NF-L / NEFL Antibody LS-C61820
LifeSpan Biosciences
catalog: LS-C61820

chicken polyclonal
reactivity: human, bovine
application: western blot, immunocytochemistry

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NF-L / NEFL Antibody LS-C16793
LifeSpan Biosciences
catalog: LS-C16793

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

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NF-L / NEFL Antibody LS-C121263
LifeSpan Biosciences
catalog: LS-C121263

mouse monoclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

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NF-L / NEFL Antibody LS-C122209
LifeSpan Biosciences
catalog: LS-C122209

mouse monoclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

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NF-L / NEFL Antibody LS-C124243
LifeSpan Biosciences
catalog: LS-C124243

chicken polyclonal
reactivity: human, mouse
application: western blot, immunohistochemistry, immunocytochemistry

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NF-L / NEFL Antibody LS-C143054
LifeSpan Biosciences
catalog: LS-C143054

chicken polyclonal
reactivity: human, rat
application: western blot, immunocytochemistry

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NF-L / NEFL Antibody LS-C149129
LifeSpan Biosciences
catalog: LS-C149129

chicken polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry

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PathPlus™ NF-L / NEFL Antibody (clone 2F11) LS-B7182
LifeSpan Biosciences
catalog: LS-B7182

mouse monoclonal (2F11)
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

NF-L / NEFL Antibody (clone 1H3) LS-C169458
LifeSpan Biosciences
catalog: LS-C169458

mouse monoclonal (1H3)
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry

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NF-L / NEFL Antibody (clone 2G10) LS-C169461
LifeSpan Biosciences
catalog: LS-C169461

mouse monoclonal (2G10)
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry

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NF-L / NEFL Antibody (clone NF-68) LS-C171214
LifeSpan Biosciences
catalog: LS-C171214

mouse monoclonal (NF-68)
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

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NF-L / NEFL Antibody (clone NF-68, Magnetic beads) LS-C171215
LifeSpan Biosciences
catalog: LS-C171215

mouse monoclonal (NF-68)
reactivity: human
conjugate: Bead
application: immunoprecipitation

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NF-L / NEFL Antibody (clone NF-68, Sepharose) LS-C171216
LifeSpan Biosciences
catalog: LS-C171216

mouse monoclonal (NF-68)
reactivity: human
application: immunoprecipitation

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NF-L / NEFL Antibody (clone OTI3G2) LS-C175341
LifeSpan Biosciences
catalog: LS-C175341

mouse monoclonal (OTI3G2)
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NEFL (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NEFL.

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NF-L / NEFL Antibody (clone OTI11F6) LS-C175551
LifeSpan Biosciences
catalog: LS-C175551

mouse monoclonal (OTI11F6)
reactivity: human
application: western blot

NF-L / NEFL Antibody (clone OTI2F5) LS-C175565
LifeSpan Biosciences
catalog: LS-C175565

mouse monoclonal (OTI2F5)
reactivity: human
application: western blot

NF-L / NEFL Antibody (clone DA2, Supernatant) LS-C188586
LifeSpan Biosciences
catalog: LS-C188586

mouse monoclonal (DA2)
reactivity: human
application: western blot, immunocytochemistry

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NF-L / NEFL Antibody (clone DA2) LS-C204554
LifeSpan Biosciences
catalog: LS-C204554

mouse monoclonal (DA2)
reactivity: human
application: western blot, immunocytochemistry

Rat spinal cord homogenate showing the major intermediate filament proteins of the nervous system (lane 1). The remaining lanes show blots of this material stained with various antibodies including NF-L / NEFL antibody (lane 4).

Cells grown from adult rat brain using method described by Evans et al. (2). Large cell in middle is stained with mouse monoclonal to NF-L clone DA2 (green). Another type of neuronal lineage cell was stained with rabbit polyclonal to alpha-internexin RPCA-a-Int (red). These cells were mitotic but had several characteristics of neurons.
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NF-L / NEFL Antibody (clone 7D1) LS-C204557
LifeSpan Biosciences
catalog: LS-C204557

mouse monoclonal (7D1)
reactivity: human
application: western blot, immunocytochemistry

Strip blots of rat spinal cord homogenate probed with two subclones of NF-L / NEFL antibody. Both subclones, one of which became the definitive antibody, stain strongly and specifically a band at 68 kDa with essentially no background.

Embryonic rat cortical cells grown in tissue culture and stained with NF-L / NEFL antibody (green) and the phosphorylated axonal form of neurofilament NF-H, using NF-H antibody (red). The perikaryal and dendritic neurofilaments in the Large cell in middle are stained with NF-L / NEFL antibody but not with the phosphorylated NF-H antibody. In contrast both antibodies stain axonal neurofilaments which therefore appear orange.
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IHC-plus™ NF-L / NEFL Antibody LS-B10376
LifeSpan Biosciences
catalog: LS-B10376

chicken polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blots of whole protein extracts of rat spinal cord (lane 1), brain stem (lane 2), cerebellum (lane 3) and cerebral cortex (lane 4).

View of mixed neuron/glial cultures stained with NF-L / NEFL antibody (red) and rabbit antibody to phosphorylated NF-H (green). The NF-L protein is assembled into neurofilaments which are found throughout the axons, dendrites and perikarya of these cells. In contrast the phosphorylated NF-H has a much more restricted expression pattern, being found only in developed axonal neurofilaments. Since both proteins are found in neurofilaments, the red and green patterns overlap, so that neurofilaments containing NF-L and phosphorylated NF-H appear yellowish. In contrast neurofilaments containing only NF-L appear red.

Anti-NF-L / NEFL antibody IHC staining of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.
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NF-L / NEFL Antibody (aa139-234) LS-C295581
LifeSpan Biosciences
catalog: LS-C295581

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot

Western blot of recombinant NF-L / NEFL. This image was taken for the unconjugated form of this product. Other forms have not been tested.

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NF-L / NEFL Antibody (aa281-396) LS-C295582
LifeSpan Biosciences
catalog: LS-C295582

domestic rabbit polyclonal
reactivity: human
application: western blot

Western Blot; Sample: Rat Cerebrum lysate; Primary Ab: 1µg/ml Rabbit Anti-Human NEFL Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody

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NF-L / NEFL Antibody (aa281-396, Biotin) LS-C300203
LifeSpan Biosciences
catalog: LS-C300203

domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot

Western Blot; Sample: Rat Cerebrum lysate; Primary Ab: 1µg/ml Rabbit Anti-Human NEFL Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody

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NF-L / NEFL Antibody (aa139-234, Biotin) LS-C300204
LifeSpan Biosciences
catalog: LS-C300204

domestic rabbit polyclonal
reactivity: human, mouse
conjugate: biotin
application: western blot

Western blot of recombinant NF-L / NEFL. This image was taken for the unconjugated form of this product. Other forms have not been tested.

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NF-L / NEFL Antibody (aa485-530) LS-C313856
LifeSpan Biosciences
catalog: LS-C313856

domestic rabbit polyclonal
reactivity: human
application: western blot

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NF-L / NEFL Antibody LS-C192234
LifeSpan Biosciences
catalog: LS-C192234

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Western blot of NEFL pAb in extracts from MCF7 cells.

Immunohistochemistry (IHC) analysis of NEFL pAb in paraffin-embedded H-tonsil tissue.
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NF-L / NEFL Antibody LS-C330877
LifeSpan Biosciences
catalog: LS-C330877

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry

Western blot analysis of extracts of MCF-7 cells, using NEFL antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.

Immunohistochemistry of paraffin-embedded human breast cancer using NEFL antibodyat dilution of 1:200 (40x lens).

Immunofluorescence analysis of HeLa cells using NEFL antibody. Blue: DAPI for nuclear staining.
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IHC-plus™ NF-L / NEFL Antibody (aa295-344) LS-B12562
LifeSpan Biosciences
catalog: LS-B12562

domestic rabbit polyclonal
reactivity: human
application: immunohistochemistry, immunohistochemistry - paraffin section

Human Brain, Cerebellum: Formalin-Fixed, Paraffin-Embedded (FFPE)
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NF-L / NEFL Antibody (Internal) LS-C358819
LifeSpan Biosciences
catalog: LS-C358819

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Western blot analysis of NEFL expression in Jurkat (A); NIH3T3 (B) whole cell lysates.

Immunohistochemical analysis of NEFL staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
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NF-L / NEFL Antibody (Internal) LS-C384855
LifeSpan Biosciences
catalog: LS-C384855

domestic rabbit polyclonal
reactivity: human
application: ELISA, immunohistochemistry

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NF-L / NEFL Antibody (C-terminal) LS-C386871
LifeSpan Biosciences
catalog: LS-C386871

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA

Western blot of NF-L antibody
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