NEFM / NF-M Antibody LS-C16816
LifeSpan Biosciences
catalog: LS-C16816
mouse monoclonal
reactivity: human, bovine
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry, radioimmunoassay, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
quantity:
price:
to the supplier
NEFM / NF-M Antibody (C-Terminus, clone 3H11) LS-C2728
LifeSpan Biosciences
catalog: LS-C2728
mouse monoclonal (3H11)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
quantity:
price:
to the supplier
NEFM + NEFH Antibody LS-C73358
LifeSpan Biosciences
catalog: LS-C73358
mouse monoclonal
reactivity: human
application: western blot, immunohistochemistry
quantity:
price:
to the supplier
NEFM / NF-M Antibody LS-C16790
LifeSpan Biosciences
catalog: LS-C16790
mouse monoclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry
quantity:
price:
to the supplier
NEFM / NF-M Antibody LS-C16801
LifeSpan Biosciences
catalog: LS-C16801
mouse monoclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
quantity:
price:
to the supplier
NEFM / NF-M Antibody (C-Terminus) LS-C16802
LifeSpan Biosciences
catalog: LS-C16802
mouse monoclonal
reactivity: human
application: western blot, immunocytochemistry
quantity:
price:
to the supplier
NEFM / NF-M Antibody LS-C16805
LifeSpan Biosciences
catalog: LS-C16805
mouse monoclonal
reactivity: human
application: western blot, immunohistochemistry
quantity:
price:
to the supplier
NEFM / NF-M Antibody (C-Terminus) LS-C16807
LifeSpan Biosciences
catalog: LS-C16807
mouse monoclonal
reactivity: human
application: western blot, immunocytochemistry
quantity:
price:
to the supplier
NEFM / NF-M Antibody LS-C16810
LifeSpan Biosciences
catalog: LS-C16810
mouse monoclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
quantity:
price:
to the supplier
NEFM / NF-M Antibody (C-Terminus) LS-C16814
LifeSpan Biosciences
catalog: LS-C16814
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry
quantity:
price:
to the supplier
NEFM / NF-M Antibody (C-Terminus) LS-C16806
LifeSpan Biosciences
catalog: LS-C16806
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
quantity:
price:
to the supplier
NEFM / NF-M Antibody (C-Terminus) LS-C2722
LifeSpan Biosciences
catalog: LS-C2722
chicken polyclonal
reactivity: human
application: western blot, immunocytochemistry
quantity:
price:
to the supplier
NEFM / NF-M Antibody LS-C37553
LifeSpan Biosciences
catalog: LS-C37553
mouse monoclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
quantity:
price:
to the supplier
NEFM / NF-M Antibody LS-C37554
LifeSpan Biosciences
catalog: LS-C37554
mouse monoclonal
reactivity: human, bovine
application: western blot, immunohistochemistry, flow cytometry
quantity:
price:
to the supplier
NEFM / NF-M Antibody LS-C37573
LifeSpan Biosciences
catalog: LS-C37573
chicken polyclonal
reactivity: human
application: immunohistochemistry
quantity:
price:
to the supplier
NEFM / NF-M Antibody (clone NF-09) LS-C45928
LifeSpan Biosciences
catalog: LS-C45928
mouse monoclonal (NF-09)
reactivity: human
application: western blot, immunocytochemistry, immunohistochemistry - paraffin section
quantity:
price:
to the supplier
NEFM / NF-M Antibody (C-Terminus) LS-C61821
LifeSpan Biosciences
catalog: LS-C61821
mouse monoclonal
reactivity: human
application: western blot, immunocytochemistry
quantity:
price:
to the supplier
NEFM / NF-M Antibody (C-Terminus) LS-C73354
LifeSpan Biosciences
catalog: LS-C73354
chicken polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry
quantity:
price:
to the supplier
NEFM / NF-M Antibody (C-Terminus) LS-C73355
LifeSpan Biosciences
catalog: LS-C73355
mouse monoclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry
quantity:
price:
to the supplier
NEFM / NF-M Antibody (C-Terminus) LS-C73384
LifeSpan Biosciences
catalog: LS-C73384
chicken polyclonal
reactivity: human
application: western blot, immunocytochemistry
quantity:
price:
to the supplier
NEFM / NF-M Antibody LS-C16811
LifeSpan Biosciences
catalog: LS-C16811
mouse monoclonal
reactivity: human, bovine
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
quantity:
price:
to the supplier
NEFM / NF-M Antibody (clone 403) LS-C121871
LifeSpan Biosciences
catalog: LS-C121871
mouse monoclonal (403)
reactivity: human
application: immunohistochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
quantity:
price:
to the supplier
NEFM / NF-M Antibody (C-Terminus) LS-C124245
LifeSpan Biosciences
catalog: LS-C124245
chicken polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry
quantity:
price:
to the supplier
NEFM / NF-M Antibody LS-C149130
LifeSpan Biosciences
catalog: LS-C149130
chicken polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry
quantity:
price:
to the supplier
PathPlus™ NEFM / NF-M Antibody (clone RNF403) LS-B7183
LifeSpan Biosciences
catalog: LS-B7183
mouse monoclonal (RNF403)
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

Anti-NEFM / NF-M antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
quantity:
price:
to the supplier
PathPlus™ NEFM / NF-M Antibody (clone RNF406) LS-B7184
LifeSpan Biosciences
catalog: LS-B7184
mouse monoclonal (RNF406)
reactivity: human
application: western blot, immunohistochemistry, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

Anti-NEFM / NF-M antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
quantity:
price:
to the supplier
NEFM / NF-M Antibody LS-C150331
LifeSpan Biosciences
catalog: LS-C150331
chicken polyclonal
reactivity: human
application: western blot, immunocytochemistry
quantity:
price:
to the supplier
NEFM / NF-M Antibody (aa429-478) LS-C169780
LifeSpan Biosciences
catalog: LS-C169780
domestic rabbit polyclonal
reactivity: human
application: western blot
quantity:
price:
to the supplier
NEFM / NF-M Antibody (C-Terminus) LS-C170429
LifeSpan Biosciences
catalog: LS-C170429
domestic rabbit polyclonal
reactivity: human
application: western blot
quantity:
price:
to the supplier
NEFM / NF-M Antibody (Lys591) LS-C177627
LifeSpan Biosciences
catalog: LS-C177627
domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot of NF-M (K591) pAb in extracts from MCF-7cells.
quantity:
price:
to the supplier
NEFM / NF-M Antibody (clone NF403) LS-C193290
LifeSpan Biosciences
catalog: LS-C193290
mouse monoclonal (NF403)
reactivity: human, bovine
application: western blot, immunohistochemistry
quantity:
price:
to the supplier
NEFM / NF-M Antibody LS-C195023
LifeSpan Biosciences
catalog: LS-C195023
chicken polyclonal
reactivity: human
application: western blot, immunocytochemistry
quantity:
price:
to the supplier
NEFM / NF-M Antibody (aa542-591) LS-C199941
LifeSpan Biosciences
catalog: LS-C199941
domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot analysis of lysate from MCF-7cells, using NF-M antibody.
quantity:
price:
to the supplier
NEFM / NF-M Antibody LS-C203130
LifeSpan Biosciences
catalog: LS-C203130
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA

Western blot of tissue lysates using Neurofilament-M Antibody, pAb, Rabbit (1 ug/ml). The signal was developed with IRDye 800 Conjugated Goat Anti-Rabbit IgG. Predicted Size: 160 kD. Observed Size: 160 kD.
quantity:
price:
to the supplier
NEFM / NF-M Antibody LS-C204560
LifeSpan Biosciences
catalog: LS-C204560
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry

Western blot of whole rat cerebellum homogenate stained with NEFM / NF-M antibody, at dilution of 1:20,000. A prominent band running with an apparent SDS-PAGE molecular weight of ~145kDa corresponds to rodent NF-M. Human, cow and bovine NF-M run a little slower, at about 160kDa.

Section of rat cerebral cortex stained with NEFM / NF-M antibody (red), which reveals the perikarya of pyramidal neurons and dendrites and axons surrounding them. The green channel shows staining with a monoclonal antibody to the beta-adrenergic receptor kinase 1.
quantity:
price:
to the supplier
NEFM / NF-M Antibody (clone 3H11) LS-C204561
LifeSpan Biosciences
catalog: LS-C204561
mouse monoclonal (3H11)
reactivity: human
application: western blot, immunocytochemistry

Rat spinal cord homogenate showing the major intermediate filament proteins of the nervous system (lane 1). The remaining lanes show blots of this material stained with various antibodies including NEFM / NF-M antibody (lane 3).

Culture of adult neural cells grown as described (2). Mature neurons can be identified by their morphology and because they stain strongly with antibodies to NF-L, NF-M and NF-H. The surrounding stellate red cells are stained with rabbit polyclonal Rat facial nucleus. These are apparently mitotic neuronal progenitor cells and express many other neuronal markers.
quantity:
price:
to the supplier
NEFM / NF-M Antibody LS-C204562
LifeSpan Biosciences
catalog: LS-C204562
chicken polyclonal
reactivity: human
application: western blot, immunocytochemistry

Western blots of homogenates of SH-SY5Y cells, a human neuroblastoma cell line. Lane A shows blotting with NEFM / NF-M antibody, which reveals a strong NF-M band at ~150kDa. Lanes B and C are homogenates of SH-SY5Y cells which were treated with maitotoxin to activate caspase family enzymes (ref. 2). Now a ~100kDa band is seen in addition o the major NF-M band. This corresponds to the C-terminal segment of NF-M which is an in vivo calpain degradation product of human NF-M.

View of mixed neuron/glial cultures stained with NEFM / NF-M antibody (red). The NF-M protein is assembled into neurofilaments which are found throughout the axons, dendrites and perikarya of these cells.
quantity:
price:
to the supplier
NEFM / NF-M Antibody (aa105-287) LS-C295580
LifeSpan Biosciences
catalog: LS-C295580
domestic rabbit polyclonal
reactivity: human
application: western blot

Western Blot; Sample: Mouse Spinal cord lysate; Primary Ab: 1µg/ml Rabbit Anti-Human NEF3 Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody

quantity:
price:
to the supplier
PathPlus™ NEFM / NF-M Antibody (aa250-263) LS-B11174
LifeSpan Biosciences
catalog: LS-B11174
domestic goat polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

NEFM antibody (0.03 ug/ml) staining of Mouse Brain lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-NEFM / NF-M antibody IHC staining of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
quantity:
price:
to the supplier
NEFM / NF-M Antibody (clone OTI1A4) LS-C338179
LifeSpan Biosciences
catalog: LS-C338179
mouse monoclonal (OTI1A4)
reactivity: human
application: western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NEFM (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NEFM.
quantity:
price:
to the supplier
NEFM / NF-M Antibody (clone OTI2C4) LS-C338193
LifeSpan Biosciences
catalog: LS-C338193
mouse monoclonal (OTI2C4)
reactivity: human
application: western blot, immunocytochemistry

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NEFM (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NEFM.

Anti-NEFM mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NEFM.
quantity:
price:
to the supplier
NEFM / NF-M Antibody (aa413-773, clone OTI2G3) LS-C339738
LifeSpan Biosciences
catalog: LS-C339738
mouse monoclonal (OTI2G3)
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NEFM (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NEFM.

IHC of paraffin-embedded Human skin tissue using anti-NEFM mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
quantity:
price:
to the supplier
NEFM / NF-M Antibody (aa413-773, clone OTI5H4) LS-C339770
LifeSpan Biosciences
catalog: LS-C339770
mouse monoclonal (OTI5H4)
reactivity: human
application: western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NEFM (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NEFM.
quantity:
price:
to the supplier
NEFM / NF-M Antibody (aa413-773, clone OTI5B7) LS-C339771
LifeSpan Biosciences
catalog: LS-C339771
mouse monoclonal (OTI5B7)
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NEFM (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NEFM.

IHC of paraffin-embedded Human skin tissue using anti-NEFM mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
quantity:
price:
to the supplier
NEFM / NF-M Antibody (clone RMO 14.9) LS-C354909
LifeSpan Biosciences
catalog: LS-C354909
mouse monoclonal (RMO 14.9)
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry
quantity:
price:
to the supplier
NEFM / NF-M Antibody (Internal) LS-C384857
LifeSpan Biosciences
catalog: LS-C384857
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA
quantity:
price:
to the supplier