IHC-plus™ NEDD8 Antibody (aa20-32) LS-B4950
LifeSpan Biosciences
catalog: LS-B4950
mouse monoclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section

Anti-NEDD8 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
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IHC-plus™ NEDD8 Antibody (aa10-59) LS-B6641
LifeSpan Biosciences
catalog: LS-B6641
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Anti-NEDD8 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.

Western blot analysis of lysates from Jurkat cells, using NEDD8 Antibody. The lane on the right is blocked with the synthesized peptide.
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NEDD8 Antibody (N-Terminus) LS-C9223
LifeSpan Biosciences
catalog: LS-C9223
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA
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NEDD8 Antibody LS-C9225
LifeSpan Biosciences
catalog: LS-C9225
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA
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NEDD8 Antibody (N-Terminus) LS-C37486
LifeSpan Biosciences
catalog: LS-C37486
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section

Western blot of NEDD8

IHC of paraffin-embedded human colon carcinoma

Flow cytometry of untreated HeLa cells
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NEDD8 Antibody (N-Terminus) LS-C94349
LifeSpan Biosciences
catalog: LS-C94349
domestic sheep polyclonal
reactivity: human, mouse
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
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NEDD8 Antibody (aa1-32) LS-C98774
LifeSpan Biosciences
catalog: LS-C98774
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Western blot of NEDD8 polyclonal antibody in HeLa cell lysate. NEDD8 (arrow) was detected using purified antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
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NEDD8 Antibody (aa2-77) LS-C144032
LifeSpan Biosciences
catalog: LS-C144032
mouse monoclonal
reactivity: human
application: western blot
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NEDD8 Antibody (clone 1A7) LS-C169462
LifeSpan Biosciences
catalog: LS-C169462
mouse monoclonal (1A7)
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry
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NEDD8 Antibody (Asp21) LS-C176339
LifeSpan Biosciences
catalog: LS-C176339
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Western blot of NEDD8 (D21) pAb in extracts from Jurkat cells.

IHC of NEDD8 (D21) pAb in paraffin-embedded human lung carcinoma tissue.
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NEDD8 Antibody LS-C179916
LifeSpan Biosciences
catalog: LS-C179916
domestic rabbit polyclonal
reactivity: human
application: western blot, immunoprecipitation
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NEDD8 Antibody (aa1-32, APC) LS-C280018
LifeSpan Biosciences
catalog: LS-C280018
domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA, immunohistochemistry
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NEDD8 Antibody (aa1-32, AP) LS-C280019
LifeSpan Biosciences
catalog: LS-C280019
domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA, immunohistochemistry
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NEDD8 Antibody (aa1-32, Biotin) LS-C280020
LifeSpan Biosciences
catalog: LS-C280020
domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA, immunohistochemistry
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NEDD8 Antibody (aa1-32, Azide-free, HRP) LS-C280021
LifeSpan Biosciences
catalog: LS-C280021
domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA, immunohistochemistry
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NEDD8 Antibody (aa1-32, FITC) LS-C280022
LifeSpan Biosciences
catalog: LS-C280022
domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA, immunohistochemistry
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NEDD8 Antibody (aa1-32, PE) LS-C280023
LifeSpan Biosciences
catalog: LS-C280023
domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA, immunohistochemistry
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IHC-plus™ NEDD8 Antibody (clone 5B8) LS-B11079
LifeSpan Biosciences
catalog: LS-B11079
mouse monoclonal (5B8)
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section

Anti-NEDD8 antibody IHC staining of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
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NEDD8 Antibody LS-C192417
LifeSpan Biosciences
catalog: LS-C192417
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

Western blot of NEDD8 pAb in extracts from COS1, Jurkat cells and mouse brain tissue.
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NEDD8 Antibody (aa1-32) LS-C328012
LifeSpan Biosciences
catalog: LS-C328012
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry
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NEDD8 Antibody LS-C331019
LifeSpan Biosciences
catalog: LS-C331019
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

Western blot analysis of extracts of mouse brain, using NEDD8 antibody. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.
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NEDD8 Antibody LS-C331310
LifeSpan Biosciences
catalog: LS-C331310
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry

Immunofluorescence analysis of HeLa cells.

Western blot analysis of extracts of various cell lines, using NEDD8 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 60s.

Immunohistochemistry of paraffin-embedded human esophagus using NEDD8 antibodyat dilution of 1:100 (40x lens).
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NEDD8 Antibody (N-Terminus) LS-C352565
LifeSpan Biosciences
catalog: LS-C352565
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunoprecipitation, immunohistochemistry - paraffin section

Western blot analysis of NEDD8 expression in BJAB (A); K562 (B); HeLa (C) whole cell lysates.

Immunohistochemical analysis of NEDD8 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
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NEDD8 Antibody (aa1-81) LS-C377416
LifeSpan Biosciences
catalog: LS-C377416
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA

Western blot. All lanes: NEDD8 antibody at 8 ug/ml. Lane 1: mouse heart tissue. Lane 2: mouse spleen tissue. Secondary antibody: Goat polyclonal to rabbit at 1:10000 dilution. Predicted band size: 9 kDa. Observed band size: 9 kDa.
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NEDD8 Antibody (aa1-81) LS-C377417
LifeSpan Biosciences
catalog: LS-C377417
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section

Western blot. All lanes: NEDD8 antibody at 16 ug/ml. Lane 1: mouse heart tissue. Lane 2: mouse spleen tissue. Secondary antibody: Goat polyclonal to rabbit at 1:10000 dilution. Predicted band size: 9 kDa. Observed band size: 9 kDa Immunohistochemistry.

Immunohistochemistry of paraffin-embedded human breast cancer using antibody at 1:100 dilution.
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NEDD8 Antibody (10-90 aa, N-terminal) LS-C384806
LifeSpan Biosciences
catalog: LS-C384806
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry

Western blot of Neddylin antibody
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