ACLY / ATP Citrate Lyase Antibody LS-C109477
LifeSpan Biosciences
catalog: LS-C109477

domestic rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry

Immunofluorescence of methanol-fixed HeLa, using ATP citrate lyase antibody at 1:200 dilution.

Sample (30 ug of whole cell lysate). A: Hela. B: Raji. 7.5% SDS PAGE. ACLY antibody diluted at 1:1000.
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ACLY / ATP Citrate Lyase Antibody (aa451-500) LS-C110717
LifeSpan Biosciences
catalog: LS-C110717

domestic rabbit polyclonal
reactivity: human
application: western blot

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ACLY / ATP Citrate Lyase Antibody (aa575-624) LS-C110718
LifeSpan Biosciences
catalog: LS-C110718

domestic rabbit polyclonal
reactivity: human
application: western blot

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ACLY / ATP Citrate Lyase Antibody (aa420-469) LS-C117739
LifeSpan Biosciences
catalog: LS-C117739

domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot analysis of lysates from COS7 cells, treated with Calyculin 50nM 30', using ATP-Citrate Lyase Antibody. The lane on the right is blocked with the synthesized peptide.
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ACLY / ATP Citrate Lyase Antibody (clone 5F8D11) LS-C169149
LifeSpan Biosciences
catalog: LS-C169149

mouse monoclonal (5F8D11)
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section

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ACLY / ATP Citrate Lyase Antibody (C-Terminus) LS-C178323
LifeSpan Biosciences
catalog: LS-C178323

mouse monoclonal
reactivity: human
application: western blot, immunocytochemistry, flow cytometry

ACLY (C-terminus) antibody at 1/1000 dilution Lane1: K562 whole cell lysate 40 ug/lane Lane2: HeLa whole cell lysate 40 ug/lane.

Immunocytochemistry of HeLa cells using anti- ATP-Citrate Lyase (C-terminus) antibody diluted 1:150.

Flow Cytometry analysis of HeLa cells stained with ATP-Citrate Lyase (red, 1/100 dilution), followed by FITC-conjugated goat anti-mouse IgG. Black line histogram represents the isotype control, normal mouse IgG.
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ACLY / ATP Citrate Lyase Antibody LS-C184984
LifeSpan Biosciences
catalog: LS-C184984

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA

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ACLY / ATP Citrate Lyase Antibody LS-C184985
LifeSpan Biosciences
catalog: LS-C184985

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA

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ACLY / ATP Citrate Lyase Antibody (Biotin) LS-C184986
LifeSpan Biosciences
catalog: LS-C184986

domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA

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ACLY / ATP Citrate Lyase Antibody (phospho-Ser454) LS-C198654
LifeSpan Biosciences
catalog: LS-C198654

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using ATP-Citrate Lyase (Phospho-Ser454) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from COS7 cells treated with Calyculin 50nM 30', using ATP-Citrate Lyase (Phospho-Ser454) Antibody. The lane on the right is blocked with the phospho peptide.
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IHC-plus™ ACLY / ATP Citrate Lyase Antibody (clone 3G8) LS-B9467
LifeSpan Biosciences
catalog: LS-B9467

mouse monoclonal (3G8)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Anti-ACLY mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ACLY.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACLY (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ACLY.

Human, Prostate: Formalin-Fixed Paraffin-Embedded (FFPE)
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ACLY / ATP Citrate Lyase Antibody (aa1051-1101) LS-C290518
LifeSpan Biosciences
catalog: LS-C290518

domestic rabbit polyclonal
reactivity: human
application: western blot

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ACLY / ATP Citrate Lyase Antibody (phospho-Thr447) LS-C291482
LifeSpan Biosciences
catalog: LS-C291482

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot

Western blot analysis of lysates from COS7 cells treated with Calyculin, using phospho-ATP-citrate synthase (Phospho-Ser455) antibody.
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ACLY / ATP Citrate Lyase Antibody (Internal) LS-C358894
LifeSpan Biosciences
catalog: LS-C358894

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot analysis of ACLY expression in Jurkat (A); HeLa (B) whole cell lysates.

Immunohistochemical analysis of ACLY staining in human liver formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Immunofluorescent analysis of ACLY staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 ??C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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ACLY / ATP Citrate Lyase Antibody (390-470 aa) LS-C381273
LifeSpan Biosciences
catalog: LS-C381273

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, ELISA, immunohistochemistry

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ACLY / ATP Citrate Lyase Antibody (390-470 aa) LS-C382209
LifeSpan Biosciences
catalog: LS-C382209

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunocytochemistry

Western blot of ATP-citrate synthase antibody
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