IHC-plus™ NDN / Necdin Antibody (clone 1B3) LS-B6103
LifeSpan Biosciences
catalog: LS-B6103

mouse monoclonal (1B3)
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot of NDN expression in transfected 293T cell line by NDN monoclonal antibody.

Immunofluorescence of monoclonal antibody to NDN on HeLa cell. [antibody concentration 10 ug/ml]

Detection limit for recombinant GST tagged NDN is approximately 0.03 ng/ml as a capture antibody.
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NDN / Necdin Antibody (aa179-228) LS-C111898
LifeSpan Biosciences
catalog: LS-C111898

domestic rabbit polyclonal
reactivity: human
application: western blot

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NDN / Necdin Antibody (aa43-71) LS-C164787
LifeSpan Biosciences
catalog: LS-C164787

domestic rabbit polyclonal
reactivity: human
application: western blot, flow cytometry

NDN Antibody western blot of Ramos cell line lysates (35 ug/lane). The NDN antibody detected the NDN protein (arrow).

NDN Antibody flow cytometry of Ramos cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
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NDN / Necdin Antibody (aa43-71, AP) LS-C223415
LifeSpan Biosciences
catalog: LS-C223415

domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA, flow cytometry

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NDN / Necdin Antibody (aa43-71, APC) LS-C223417
LifeSpan Biosciences
catalog: LS-C223417

domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA, flow cytometry

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NDN / Necdin Antibody (aa43-71, Biotin) LS-C223418
LifeSpan Biosciences
catalog: LS-C223418

domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA, flow cytometry

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NDN / Necdin Antibody (aa43-71, FITC) LS-C223419
LifeSpan Biosciences
catalog: LS-C223419

domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA, flow cytometry

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NDN / Necdin Antibody (aa43-71, Azide-free, HRP) LS-C223420
LifeSpan Biosciences
catalog: LS-C223420

domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA, flow cytometry

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NDN / Necdin Antibody (aa43-71, PE) LS-C223422
LifeSpan Biosciences
catalog: LS-C223422

domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA, flow cytometry

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NDN / Necdin Antibody (aa43-71) LS-C323259
LifeSpan Biosciences
catalog: LS-C323259

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, flow cytometry

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NDN / Necdin Antibody (clone OTI3A12) LS-C338301
LifeSpan Biosciences
catalog: LS-C338301

mouse monoclonal (OTI3A12)
reactivity: human
application: western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NDN (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NDN.
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NDN / Necdin Antibody (clone OTI5D1) LS-C338303
LifeSpan Biosciences
catalog: LS-C338303

mouse monoclonal (OTI5D1)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NDN (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NDN.

IHC of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-NDN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min).

Immunofluorescent staining of HeLa cells using anti-NDN mouse monoclonal antibody.
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NDN / Necdin Antibody (clone OTI1B1) LS-C338306
LifeSpan Biosciences
catalog: LS-C338306

mouse monoclonal (OTI1B1)
reactivity: human
application: western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NDN (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NDN.
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NDN / Necdin Antibody (aa75-109) LS-C344470
LifeSpan Biosciences
catalog: LS-C344470

domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot of lysate from human heart tissue lysate with NDN Antibody. Antibody was diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20 ug.
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