NCK1 / NCK Antibody (Gly300) LS-C37473
LifeSpan Biosciences
catalog: LS-C37473
domestic rabbit monoclonal
reactivity: human
application: western blot, ELISA, immunoprecipitation
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NCK1 / NCK Antibody (aa1-254) LS-C37474
LifeSpan Biosciences
catalog: LS-C37474
domestic rabbit polyclonal
reactivity: human
application: western blot, immunoprecipitation
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IHC-plus™ NCK1 / NCK Antibody LS-B7146
LifeSpan Biosciences
catalog: LS-B7146
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Anti-NCK1 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.

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NCK1 / NCK Antibody (aa52-101) LS-C151733
LifeSpan Biosciences
catalog: LS-C151733
domestic rabbit polyclonal
reactivity: human
application: western blot
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NCK1 / NCK Antibody LS-C155317
LifeSpan Biosciences
catalog: LS-C155317
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Sample (30 ug of whole cell lysate) A: K562 10% SDS PAGE NCK1 antibody diluted at 1:1000

IHC of paraffin-embedded HeLa xenograft, using NCK1 antibody at 1:500 dilution.

Immunofluorescence of paraformaldehyde-fixed A431, using NCK1 antibody at 1:500 dilution.
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NCK1 / NCK Antibody (aa3-29) LS-C164772
LifeSpan Biosciences
catalog: LS-C164772
domestic rabbit polyclonal
reactivity: human
application: western blot, flow cytometry

Western blot of NCK1 Antibody in HeLa cell line lysates (35 ug/lane). NCK1 (arrow) was detected using the purified antibody.

NCK1 Antibody flow cytometry of MDA-MB231 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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NCK1 / NCK Antibody (aa63-92) LS-C164773
LifeSpan Biosciences
catalog: LS-C164773
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Western blot of NCK1 (arrow) using rabbit polyclonal NCK1 Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the NCK1 gene.

NCK1 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human breast carcinoma followed by peroxidase-conjugated secondary antibody and DAB staining.
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NCK1 / NCK Antibody (clone 5B7) LS-C169076
LifeSpan Biosciences
catalog: LS-C169076
mouse monoclonal (5B7)
reactivity: human
application: western blot, ELISA, immunohistochemistry, flow cytometry
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NCK1 / NCK Antibody (clone 1A1) LS-C197753
LifeSpan Biosciences
catalog: LS-C197753
mouse monoclonal (1A1)
reactivity: human
application: western blot, ELISA

Western Blot analysis of NCK1 expression in transfected 293T cell line by NCK1 monoclonal antibody (M01), clone 1A1.Lane 1: NCK1 transfected lysate(42.9 KDa).Lane 2: Non-transfected lysate.

Detection limit for recombinant GST tagged NCK1 is approximately 0.1 ng/ml as a capture antibody.
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NCK1 / NCK Antibody (aa63-92, AP) LS-C223406
LifeSpan Biosciences
catalog: LS-C223406
domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA, immunohistochemistry
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NCK1 / NCK Antibody (aa63-92, APC) LS-C223409
LifeSpan Biosciences
catalog: LS-C223409
domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA, immunohistochemistry
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NCK1 / NCK Antibody (aa63-92, Biotin) LS-C223410
LifeSpan Biosciences
catalog: LS-C223410
domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA, immunohistochemistry
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NCK1 / NCK Antibody (aa63-92, FITC) LS-C223413
LifeSpan Biosciences
catalog: LS-C223413
domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA, immunohistochemistry
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NCK1 / NCK Antibody (aa63-92, Azide-free, HRP) LS-C223414
LifeSpan Biosciences
catalog: LS-C223414
domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA, immunohistochemistry
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NCK1 / NCK Antibody (aa63-92, PE) LS-C223416
LifeSpan Biosciences
catalog: LS-C223416
domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA, immunohistochemistry
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NCK1 / NCK Antibody (aa3-29, AP) LS-C225968
LifeSpan Biosciences
catalog: LS-C225968
domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA
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NCK1 / NCK Antibody (aa3-29, APC) LS-C225969
LifeSpan Biosciences
catalog: LS-C225969
domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA
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NCK1 / NCK Antibody (aa3-29, Biotin) LS-C225970
LifeSpan Biosciences
catalog: LS-C225970
domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA
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NCK1 / NCK Antibody (aa3-29, FITC) LS-C225971
LifeSpan Biosciences
catalog: LS-C225971
domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA
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NCK1 / NCK Antibody (aa3-29, Azide-free, HRP) LS-C225973
LifeSpan Biosciences
catalog: LS-C225973
domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA
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NCK1 / NCK Antibody (aa3-29, PE) LS-C225974
LifeSpan Biosciences
catalog: LS-C225974
domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA
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NCK1 / NCK Antibody LS-C192324
LifeSpan Biosciences
catalog: LS-C192324
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

Western blot of NCK1 pAb in extracts from mouse brain tissue.
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NCK1 / NCK Antibody (aa3-29) LS-C328003
LifeSpan Biosciences
catalog: LS-C328003
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA
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NCK1 / NCK Antibody (aa63-92) LS-C329877
LifeSpan Biosciences
catalog: LS-C329877
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry
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NCK1 / NCK Antibody LS-C330807
LifeSpan Biosciences
catalog: LS-C330807
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

Western blot analysis of extracts of various cell lines, using NCK1 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 30s.
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NCK1 / NCK Antibody LS-C331196
LifeSpan Biosciences
catalog: LS-C331196
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry

Western blot analysis of extracts of various cell lines.

Immunofluorescence analysis of A549 cells.
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NCK1 / NCK Antibody (aa2-377) LS-C374632
LifeSpan Biosciences
catalog: LS-C374632
domestic rabbit polyclonal
reactivity: human
application: western blot

Western Blot; Sample: Human Lung lysate; Primary Ab: 1µg/ml Rabbit Anti-Human NCK1 Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody

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NCK1 / NCK Antibody (aa58-377) LS-C376206
LifeSpan Biosciences
catalog: LS-C376206
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section

Western blot. All lanes: Cytoplasmic protein NCK1 antibody at 4 ug/ml+rat liver tissue. Secondary antibody: Goat polyclonal to rabbit at 1:10000 dilution. Predicted band size: 43 kDa. Observed band size: 43 kDa.

Immunohistochemistry of paraffin-embedded human tonsil using antibody at 1:100 dilution.
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