IHC-plus™ P40PHOX / NCF4 Antibody (aa228-238) LS-B3604
LifeSpan Biosciences
catalog: LS-B3604

domestic goat polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

HEK293 overexpressing P40PHOX (RC201191) and probed with (mock transfection in first lane).

Anti-NCF4 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.
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P40PHOX / NCF4 Antibody (aa120-169) LS-C117767
LifeSpan Biosciences
catalog: LS-C117767

domestic rabbit polyclonal
reactivity: human
application: immunohistochemistry

Immunohistochemistry analysis of paraffin-embedded human ovary tissue, using p40 phox Antibody. The picture on the right is blocked with the synthesized peptide.
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P40PHOX / NCF4 Antibody (C-Terminus) LS-C9419
LifeSpan Biosciences
catalog: LS-C9419

domestic rabbit polyclonal
reactivity: human
application: western blot

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P40PHOX / NCF4 Antibody (aa51-100) LS-C80786
LifeSpan Biosciences
catalog: LS-C80786

domestic rabbit polyclonal
reactivity: human
application: western blot

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P40PHOX / NCF4 Antibody (aa260-289) LS-C100269
LifeSpan Biosciences
catalog: LS-C100269

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, flow cytometry, immunohistochemistry - paraffin section

Western blot of NCF4 antibody in K562 cell line lysates (35 ug/lane). NCF4 (arrow) was detected using the purified antibody.

Formalin-fixed and paraffin-embedded human lymphoma reacted with NCF4 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

NCF4 Antibody flow cytometry of K562 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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P40PHOX / NCF4 Antibody (aa216-265) LS-C102201
LifeSpan Biosciences
catalog: LS-C102201

domestic rabbit polyclonal
reactivity: human
application: western blot

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P40PHOX / NCF4 Antibody (aa267-316) LS-C117085
LifeSpan Biosciences
catalog: LS-C117085

domestic rabbit polyclonal
reactivity: human
application: western blot

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P40PHOX / NCF4 Antibody LS-C155298
LifeSpan Biosciences
catalog: LS-C155298

domestic rabbit polyclonal
reactivity: human
application: western blot

Sample (30 ug of whole cell lysate) A: Jurkat B: Raji C: K562 D: THP-1 10% SDS PAGE NCF4 antibody diluted at 1:1000
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P40PHOX / NCF4 Antibody (Arg149) LS-C176438
LifeSpan Biosciences
catalog: LS-C176438

domestic rabbit polyclonal
reactivity: human
application: immunohistochemistry, immunohistochemistry - paraffin section

IHC of p40 phox (R149) pAb in paraffin-embedded human ovary tissue.
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P40PHOX / NCF4 Antibody (clone 3C10) LS-C197752
LifeSpan Biosciences
catalog: LS-C197752

mouse monoclonal (3C10)
reactivity: human
application: ELISA

Detection limit for recombinant GST tagged NCF4 is approximately 0.3 ng/ml as a capture antibody.
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P40PHOX / NCF4 Antibody (phospho-Thr154) LS-C199374
LifeSpan Biosciences
catalog: LS-C199374

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: immunohistochemistry, immunohistochemistry - paraffin section

Immunohistochemistry analysis of paraffin-embedded human ovary, using p40 phox (Phospho-Thr154) Antibody. The picture on the right is blocked with the phospho peptide.
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P40PHOX / NCF4 Antibody (aa132-339) LS-C314467
LifeSpan Biosciences
catalog: LS-C314467

domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot of recombinant P40PHOX / NCF4.

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P40PHOX / NCF4 Antibody (aa111-339) LS-C314469
LifeSpan Biosciences
catalog: LS-C314469

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot

Western Blot; Sample: Mouse Spleen Tissue.

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P40PHOX / NCF4 Antibody LS-C331888
LifeSpan Biosciences
catalog: LS-C331888

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry

Western blot analysis of extracts of various cells.

Immunofluorescence analysis of U20S cells.
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P40PHOX / NCF4 Antibody (Internal) LS-C356208
LifeSpan Biosciences
catalog: LS-C356208

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Western blot analysis of p40 phox expression in MCF7 (A) whole cell lysates.

Immunohistochemical analysis of p40 phox staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
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P40PHOX / NCF4 Antibody (90-170 aa) LS-C381557
LifeSpan Biosciences
catalog: LS-C381557

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: ELISA, immunohistochemistry

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P40PHOX / NCF4 Antibody (90-170 aa) LS-C385279
LifeSpan Biosciences
catalog: LS-C385279

domestic rabbit polyclonal
reactivity: human
application: ELISA, immunohistochemistry

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