IHC-plus™ ATF1 Antibody (N-Terminus) LS-B1511
LifeSpan Biosciences
catalog: LS-B1511
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Anti-ATF1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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IHC-plus™ ATF1 Antibody (clone 3E7) LS-B5378
LifeSpan Biosciences
catalog: LS-B5378
mouse monoclonal (3E7)
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section

Western blot of ATF1 expression in transfected 293T cell line by ATF1 monoclonal antibody (M03), clone 3E7.

Detection limit for recombinant GST tagged ATF1 is 0.3 ng/ml as a capture antibody.

Western blot of ATF1 over-expressed 293 cell line, cotransfected with ATF1 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with ATF1 monoclonal antibody (3E7). GAPDH (36.1 kD) used as specificity and loading control.
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ATF1 Antibody LS-C63598
LifeSpan Biosciences
catalog: LS-C63598
domestic goat polyclonal
reactivity: human
application: western blot
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ATF1 Antibody (aa31-80) LS-C117593
LifeSpan Biosciences
catalog: LS-C117593
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using ATF1 Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from COLO cells, using ATF1 Antibody. The lane on the right is blocked with the synthesized peptide.
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ATF1 Antibody LS-C136891
LifeSpan Biosciences
catalog: LS-C136891
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA
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IHC-plus™ ATF1 Antibody (aa179-228) LS-B6733
LifeSpan Biosciences
catalog: LS-B6733
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Anti-ATF1 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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IHC-plus™ ATF1 Antibody (C-Terminus) LS-B6808
LifeSpan Biosciences
catalog: LS-B6808
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Anti-ATF1 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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ATF1 Antibody (aa193-219) LS-C156560
LifeSpan Biosciences
catalog: LS-C156560
domestic rabbit polyclonal
reactivity: human
application: western blot, flow cytometry

Western blot of ATF1 Antibody in Jurkat cell line lysates (35 ug/lane). ATF1 (arrow) was detected using the purified antibody.

ATF1 Antibody flow cytometry of Jurkat cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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ATF1 Antibody (Thr209) LS-C176744
LifeSpan Biosciences
catalog: LS-C176744
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry

Western blot of ATF1 (T209) pAb in extracts from HepG2 cells.
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IHC-plus™ ATF1 Antibody (aa176-225) LS-B9140
LifeSpan Biosciences
catalog: LS-B9140
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Human, Skin: Formalin-Fixed Paraffin-Embedded (FFPE)

Immunofluorescence analysis of HUVEC cells, using ATF1 Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from HepG2, COLO205, and HeLa cells, using ATF1 Antibody. The lane on the right is blocked with the synthesized peptide.
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ATF1 Antibody (phospho-Ser63) LS-C198647
LifeSpan Biosciences
catalog: LS-C198647
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot

Western blot analysis of lysates from HT29 cells treated with Insulin 0.01U/ML 15', using ATF1 (Phospho-Ser63) Antibody. The lane on the right is blocked with the phospho peptide.
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ATF1 Antibody (aa70-119) LS-C205641
LifeSpan Biosciences
catalog: LS-C205641
domestic rabbit polyclonal
reactivity: human
application: western blot
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ATF1 Antibody (aa193-219, AP) LS-C208053
LifeSpan Biosciences
catalog: LS-C208053
domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA, flow cytometry
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ATF1 Antibody (aa193-219, APC) LS-C208054
LifeSpan Biosciences
catalog: LS-C208054
domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA, flow cytometry
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ATF1 Antibody (aa193-219, Biotin) LS-C208055
LifeSpan Biosciences
catalog: LS-C208055
domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA, flow cytometry
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ATF1 Antibody (aa193-219, FITC) LS-C208056
LifeSpan Biosciences
catalog: LS-C208056
domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA, flow cytometry
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ATF1 Antibody (aa193-219, Azide-free, HRP) LS-C208057
LifeSpan Biosciences
catalog: LS-C208057
domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA, flow cytometry
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ATF1 Antibody (aa193-219, PE) LS-C208058
LifeSpan Biosciences
catalog: LS-C208058
domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA, flow cytometry
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ATF1 Antibody (aa1-50) LS-C289740
LifeSpan Biosciences
catalog: LS-C289740
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunoprecipitation
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ATF1 Antibody (aa171-221) LS-C289741
LifeSpan Biosciences
catalog: LS-C289741
domestic rabbit polyclonal
reactivity: human
application: immunohistochemistry, immunoprecipitation
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ATF1 Antibody (aa221-271) LS-C289743
LifeSpan Biosciences
catalog: LS-C289743
domestic rabbit polyclonal
reactivity: human
application: western blot, immunoprecipitation
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ATF1 Antibody (aa3-213) LS-C292616
LifeSpan Biosciences
catalog: LS-C292616
domestic rabbit polyclonal
reactivity: human
application: western blot

Western Blot; Sample: Lane1: Rat Blood Cells; Lane2: Porcine Blood Cells.

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ATF1 Antibody (aa2-240) LS-C292617
LifeSpan Biosciences
catalog: LS-C292617
domestic rabbit polyclonal
reactivity: human, rat
application: western blot

Western Blot; Sample: Mouse Lung lysate; Primary Ab: 2µg/ml Rabbit Anti-Rat ATF1 Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody

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ATF1 Antibody LS-C309542
LifeSpan Biosciences
catalog: LS-C309542
domestic rabbit polyclonal
reactivity: human
application: western blot
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ATF1 Antibody (aa193-219) LS-C319652
LifeSpan Biosciences
catalog: LS-C319652
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, flow cytometry
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ATF1 Antibody LS-C334292
LifeSpan Biosciences
catalog: LS-C334292
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

Western blot analysis of extracts of various cell lines.
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ATF1 Antibody (clone OTI5D10) LS-C340369
LifeSpan Biosciences
catalog: LS-C340369
mouse monoclonal (OTI5D10)
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ATF1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ATF1.

IHC of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-ATF1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
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ATF1 Antibody (clone OTI8G3) LS-C340371
LifeSpan Biosciences
catalog: LS-C340371
mouse monoclonal (OTI8G3)
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ATF1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ATF1.

IHC of paraffin-embedded Carcinoma of Human lung tissue using anti-ATF1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
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ATF1 Antibody (aa1-271) LS-C343989
LifeSpan Biosciences
catalog: LS-C343989
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

ATF1 antibody Western blot. All lanes: Anti ATF1 at 0.5 ug/ml. Lane 1: Rat Spleen Tissue Lysate at 50 ug. Lane 2: HELA Whole Cell Lysate at 40 ug. Lane 3: COLO320 Whole Cell Lysate at 40 ug. Predicted band size: 29 kD . Observed band size: 29 kD.

ATF1 antibody IHC-paraffin: Human Intestinal Cancer Tissue.
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ATF1 Antibody (C-Terminus) LS-C351857
LifeSpan Biosciences
catalog: LS-C351857
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

Western blot analysis of ATF1 expression in HeLa (A); KNRK (B); A431 (C); SP2/0 (D); mouse brain (E); H9C2 (F) whole cell lysates.

Immunohistochemical analysis of ATF1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Immunofluorescent analysis of ATF1 staining in KNRK cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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ATF1 Antibody (phospho-Ser63) LS-C368742
LifeSpan Biosciences
catalog: LS-C368742
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunoprecipitation

Western blot analysis of ATF1 (pS63) expression in HEK293T (A); NIH3T3 (B); mouse kidney (C) whole cell lysates.
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ATF1 Antibody (aa3-245) LS-C374104
LifeSpan Biosciences
catalog: LS-C374104
domestic rabbit polyclonal
reactivity: human, mouse
application: western blot


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ATF1 Antibody (aa1-210) LS-C375280
LifeSpan Biosciences
catalog: LS-C375280
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section
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ATF1 Antibody (1-80 aa) LS-C380888
LifeSpan Biosciences
catalog: LS-C380888
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, ELISA

Western blot of Phospho-ATF-1 (S63) antibody
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ATF1 Antibody (1-80 aa) LS-C382172
LifeSpan Biosciences
catalog: LS-C382172
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry

Western blot of ATF-1 antibody
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ATF1 Antibody (150-230 aa, C-terminal) LS-C382173
LifeSpan Biosciences
catalog: LS-C382173
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry

Western blot of ATF-1 antibody
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