mouse monoclonal (4F8)
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section


Western blot of MYOC expression in transfected 293T cell line by MYOC monoclonal antibody clone 4F8.

Western blot of MYOC over-expressed 293 cell line, cotransfected with MYOC Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with MYOC monoclonal antibody clone 4F8. GAPDH ( 36.1 kD ) used as specificity and.

Detection limit for recombinant GST tagged MYOC is approximately 0.3 ng/ml as a capture antibody.
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to the supplier
domestic rabbit polyclonal
reactivity: human
application: western blot
reactivity: human
application: western blot
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry
reactivity: human
application: western blot, immunohistochemistry
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry
reactivity: human
application: western blot, immunohistochemistry


Western blot analysis of extracts of various cell lines, using MYOC antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 60s.
quantity:
price:
to the supplier