IHC-plus™ MUTYH / MYH Antibody (aa531-546) LS-B1974
LifeSpan Biosciences
catalog: LS-B1974

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Anti-MUTYH antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
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MUTYH / MYH Antibody (C-Terminus) LS-C25691
LifeSpan Biosciences
catalog: LS-C25691

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA

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MUTYH / MYH Antibody (Internal) LS-C354036
LifeSpan Biosciences
catalog: LS-C354036

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
citations: 1

Western blot analysis of MUTYH expression in K562 (A); HL60 (B); NIH3T3 (C) whole cell lysates.

Immunohistochemical analysis of MUTYH staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Immunofluorescent analysis of MUTYH staining in K562 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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MUTYH / MYH Antibody (N-Terminus) LS-C25689
LifeSpan Biosciences
catalog: LS-C25689

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA

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MUTYH / MYH Antibody (aa533-546) LS-C55134
LifeSpan Biosciences
catalog: LS-C55134

domestic goat polyclonal
reactivity: human
application: western blot

Antibody (0.1 ug/ml) staining of Human Bone Marrow lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Myosin Heavy Chain Antibody LS-C41290
LifeSpan Biosciences
catalog: LS-C41290

domestic rabbit polyclonal
reactivity: human
application: immunohistochemistry, immunocytochemistry

Hela cells stained with purified rabbit polyclonal antibody against Myosin heavy chain (poly6212), followed by Cy3 conjugated Donkey anti-rabbit IgG and DAPI.

Rat carotid artery frozen sections were stained with Poly6212, followed by anti-rabbit Alexa Fluor 594 and DAPI staining.
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MUTYH / MYH Antibody (aa151-200) LS-C119579
LifeSpan Biosciences
catalog: LS-C119579

domestic rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry

Immunofluorescence analysis of A549 cells, using MUTYH Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from HeLa and HepG2 cells, using MUTYH Antibody. The lane on the right is blocked with the synthesized peptide.
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MUTYH / MYH Antibody (aa341-367) LS-C164686
LifeSpan Biosciences
catalog: LS-C164686

domestic rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry, flow cytometry

Western blot of MUTYH Antibody in mouse lung tissue lysates (35 ug/lane). MUTYH (arrow) was detected using the purified antibody.

MUTYH Antibody flow cytometry of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Confocal immunofluorescence of MUTYH Antibody with HeLa cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
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MUTYH / MYH Antibody (Tyr176) LS-C177045
LifeSpan Biosciences
catalog: LS-C177045

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot of MYH (Y176) pAb in extracts from HepG2 cells.

IHC of MYH (Y176) pAb in paraffin-embedded human colon carcinoma tissue.
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MUTYH / MYH Antibody (clone 4D10) LS-C197721
LifeSpan Biosciences
catalog: LS-C197721

mouse monoclonal (4D10)
reactivity: human
application: western blot, ELISA, immunoprecipitation

Western Blot analysis of MUTYH expression in transfected 293T cell line by MUTYH monoclonal antibody (M01), clone 4D10.Lane 1: MUTYH transfected lysate(59.1 KDa).Lane 2: Non-transfected lysate.

Immunoprecipitation of MUTYH transfected lysate using anti-MUTYH monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with MUTYH rabbit polyclonal antibody.

Detection limit for recombinant GST tagged MUTYH is approximately 1 ng/ml as a capture antibody.
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MUTYH / MYH Antibody (aa127-176) LS-C200241
LifeSpan Biosciences
catalog: LS-C200241

domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot analysis of the lysates from HUVECcells using MUTYH antibody.
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MUTYH / MYH Antibody (C-Terminus) LS-C204259
LifeSpan Biosciences
catalog: LS-C204259

domestic goat polyclonal
reactivity: human
application: western blot

Western blot. Anti-Mutyh antibody at 1:1000 dilution. Recombinant protein at 50 ng and 293HEK transfected lysate at 100 ug per lane. Rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution.
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MUTYH / MYH Antibody LS-C331584
LifeSpan Biosciences
catalog: LS-C331584

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry

Western blot analysis of extracts of various cell lines.

Immunofluorescence analysis of U20S cells.
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MUTYH / MYH Antibody LS-C332024
LifeSpan Biosciences
catalog: LS-C332024

domestic rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry, flow cytometry

Western blot analysis of extracts of various cell lines.
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MUTYH / MYH Antibody (aa1-265) LS-C379928
LifeSpan Biosciences
catalog: LS-C379928

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section

Western blot. All lanes: A/G-specific adenine DNA glycosylase antibody at 5 ug/ml. Lane 1: HeLa whole cell lysate. Lane 2: Jurkat whole cell lysate. Secondary antibody: Goat polyclonal to rabbit at 1:10000 dilution. Predicted band size: 60 kDa. Observed band size: 60 kDa.

Immunohistochemistry of paraffin-embedded human colon cancer using antibody at 1:100 dilution.
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MUTYH / MYH Antibody (120-200 aa, Internal) LS-C384708
LifeSpan Biosciences
catalog: LS-C384708

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry

Western blot of MYH antibody
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