IHC-plus™ FOXO4 / AFX1 Antibody LS-B5285
LifeSpan Biosciences
catalog: LS-B5285

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Anti-FOXO4 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Immunocytochemistry of FOXO4 in HeLa cells with FOXO4 antibody at 10 ug/ml.

Western blot analysis of FOXO4 in HeLa cell lysate with FOXO4 antibody at (A) 0.5 and (B) 1 ug/ml.
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IHC-plus™ FOXO4 / AFX1 Antibody LS-B5287
LifeSpan Biosciences
catalog: LS-B5287

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Anti-FOXO4 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

Immunocytochemistry of FOXO4 in HeLa cells with FOXO4 antibody at 10 ug/ml.

Western blot analysis of FOXO4 in A-20 cell lysate with FOXO4 antibody at (A) 0.5 and (B) 1 ug/ml.
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IHC-plus™ FOXO4 / AFX1 Antibody (aa59-73) LS-B873
LifeSpan Biosciences
catalog: LS-B873

domestic rabbit polyclonal
reactivity: human
application: ELISA, immunohistochemistry, immunohistochemistry - paraffin section

Anti-FOXO4 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
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FOXO4 / AFX1 Antibody (phospho-Ser193) LS-C34911
LifeSpan Biosciences
catalog: LS-C34911

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, ELISA

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FOXO4 / AFX1 Antibody (phospho-Ser193) LS-C48284
LifeSpan Biosciences
catalog: LS-C48284

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot

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FOXO4 / AFX1 Antibody LS-C48387
LifeSpan Biosciences
catalog: LS-C48387

domestic rabbit polyclonal
reactivity: human
application: western blot

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FOXO4 / AFX1 Antibody (aa459-471) LS-C54870
LifeSpan Biosciences
catalog: LS-C54870

domestic goat polyclonal
reactivity: human
application: western blot, ELISA

Antibody (1 ug/ml) staining of K562 cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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FOXO4 / AFX1 Antibody (aa108-157) LS-C112273
LifeSpan Biosciences
catalog: LS-C112273

domestic rabbit polyclonal
reactivity: human
application: western blot

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FOXO4 / AFX1 Antibody (phospho-Ser197) LS-C117359
LifeSpan Biosciences
catalog: LS-C117359

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using AFX (Phospho-Ser197) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from 293 cells treated with serum, using AFX (Phospho-Ser197) Antibody. The lane on the right is blocked with the phospho peptide.
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FOXO4 / AFX1 Antibody (aa417-466) LS-C118003
LifeSpan Biosciences
catalog: LS-C118003

domestic rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry

Immunofluorescence analysis of HeLa cells, using FOXO4 Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from K562 cells, using FOXO4 Antibody. The lane on the right is blocked with the synthesized peptide.
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FOXO4 / AFX1 Antibody (phospho-Ser197) LS-C122619
LifeSpan Biosciences
catalog: LS-C122619

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry

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FOXO4 / AFX1 Antibody LS-C122620
LifeSpan Biosciences
catalog: LS-C122620

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

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FOXO4 / AFX1 Antibody (C-Terminus) LS-C134941
LifeSpan Biosciences
catalog: LS-C134941

domestic rabbit polyclonal
reactivity: human
application: western blot

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FOXO4 / AFX1 Antibody (C-Terminus) LS-C145800
LifeSpan Biosciences
catalog: LS-C145800

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot

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FOXO4 / AFX1 Antibody (Lys447) LS-C176677
LifeSpan Biosciences
catalog: LS-C176677

domestic rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry

Western blot of FoxO4 (K447) pAb in extracts from K562 cells.
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FOXO4 / AFX1 Antibody (Pro191) LS-C177631
LifeSpan Biosciences
catalog: LS-C177631

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Western blot of FoxO4 (P191) pAb in extracts from COLO205 cells.

IHC of FoxO4 (P191) pAb in paraffin-embedded human breast carcinoma tissue.
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FOXO4 / AFX1 Antibody (phospho-Ser197) LS-C177994
LifeSpan Biosciences
catalog: LS-C177994

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Western blot of p-AFX1 (S197) pAb in extracts from COLO205 cells.

IHC of p-AFX1 (S197) pAb in paraffin-embedded human brain tissue.
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FOXO4 / AFX1 Antibody (phospho-Ser262) LS-C198979
LifeSpan Biosciences
catalog: LS-C198979

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Immunohistochemistry analysis of paraffin-embedded human brain, using FOXO4 (Phospho-Ser262) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from Jurkat cells treated with starved 24h, using FOXO4 (Phospho-Ser262) Antibody. The lane on the right is blocked with the phospho peptide.
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FOXO4 / AFX1 Antibody (phospho-Thr451) LS-C198980
LifeSpan Biosciences
catalog: LS-C198980

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot

Western blot analysis of lysates from HUVEC cells treated with EGF 200ng/ml 5', using FOXO4 (Phospho-Thr451) Antibody. The lane on the right is blocked with the phospho peptide.
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FOXO4 / AFX1 Antibody (N-Terminus, AP) LS-C277420
LifeSpan Biosciences
catalog: LS-C277420

domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: ELISA, immunohistochemistry

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FOXO4 / AFX1 Antibody (N-Terminus, APC) LS-C277421
LifeSpan Biosciences
catalog: LS-C277421

domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: ELISA, immunohistochemistry

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FOXO4 / AFX1 Antibody (N-Terminus, Biotin) LS-C277422
LifeSpan Biosciences
catalog: LS-C277422

domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: ELISA, immunohistochemistry

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FOXO4 / AFX1 Antibody (N-Terminus, FITC) LS-C277423
LifeSpan Biosciences
catalog: LS-C277423

domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: ELISA, immunohistochemistry

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FOXO4 / AFX1 Antibody (N-Terminus, Azide-free, HRP) LS-C277424
LifeSpan Biosciences
catalog: LS-C277424

domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: ELISA, immunohistochemistry

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FOXO4 / AFX1 Antibody (N-Terminus, PE) LS-C277426
LifeSpan Biosciences
catalog: LS-C277426

domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: ELISA, immunohistochemistry

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FOXO4 / AFX1 Antibody (aa455-505) LS-C289144
LifeSpan Biosciences
catalog: LS-C289144

domestic rabbit polyclonal
reactivity: human
application: western blot

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FOXO4 / AFX1 Antibody (aa228-277) LS-C291232
LifeSpan Biosciences
catalog: LS-C291232

domestic rabbit polyclonal
reactivity: human
application: western blot

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FOXO4 / AFX1 Antibody (phospho-Thr451) LS-C313587
LifeSpan Biosciences
catalog: LS-C313587

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot

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FOXO4 / AFX1 Antibody (N-Terminus) LS-C330443
LifeSpan Biosciences
catalog: LS-C330443

domestic rabbit polyclonal
reactivity: human
application: ELISA, immunohistochemistry

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FOXO4 / AFX1 Antibody LS-C332480
LifeSpan Biosciences
catalog: LS-C332480

domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot analysis of extracts of various cells.
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FOXO4 / AFX1 Antibody (phospho-Ser197) LS-C335844
LifeSpan Biosciences
catalog: LS-C335844

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry

Western blot analysis of extracts from 293 cells.

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue.

Immunofluorescence staining of methanol-fixed MCF7 cells.
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FOXO4 / AFX1 Antibody (phospho-Thr451) LS-C352512
LifeSpan Biosciences
catalog: LS-C352512

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot

Western blot analysis of FOXO4 (pT451) expression in THP1 EGF-treated (A); K562 (B); Jurkat (C); NIH3T3 (D); mouse lung (E) whole cell lysates.
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FOXO4 / AFX1 Antibody (phospho-Ser197) LS-C352513
LifeSpan Biosciences
catalog: LS-C352513

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot analysis of FOXO4 (pS197) expression in HeLa (A); MM142 (B); HuT78 (C); HEK293T (D); NIH3T3 (E) whole cell lysates.

Immunohistochemical analysis of FOXO4 (pS197) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Immunofluorescent analysis of FOXO4 (pS197) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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FOXO4 / AFX1 Antibody (Internal) LS-C352514
LifeSpan Biosciences
catalog: LS-C352514

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Western blot analysis of FOXO4 expression in HeLa (A); MM142 (B); HuT78 (C); NIH3T3 (D) whole cell lysates.

Immunohistochemical analysis of FOXO4 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
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FOXO4 / AFX1 Antibody (aa415-450) LS-C356394
LifeSpan Biosciences
catalog: LS-C356394

domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot of lysate from human placenta tissue lysate, using FOXO4 antibody diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20 ug.
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FOXO4 / AFX1 Antibody (140-220 aa) LS-C380715
LifeSpan Biosciences
catalog: LS-C380715

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, ELISA, immunohistochemistry

Western blot of Phospho-FoxO4 (S197) antibody
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FOXO4 / AFX1 Antibody (390-470 aa) LS-C380980
LifeSpan Biosciences
catalog: LS-C380980

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, ELISA

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FOXO4 / AFX1 Antibody (200-280 aa) LS-C381368
LifeSpan Biosciences
catalog: LS-C381368

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, ELISA, immunohistochemistry

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FOXO4 / AFX1 Antibody (140-220 aa) LS-C383550
LifeSpan Biosciences
catalog: LS-C383550

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry

Western blot of FoxO4 antibody
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FOXO4 / AFX1 Antibody (390-470 aa) LS-C383553
LifeSpan Biosciences
catalog: LS-C383553

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunocytochemistry

Western blot of FoxO4 antibody
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FOXO4 / AFX1 Antibody (200-280 aa) LS-C383557
LifeSpan Biosciences
catalog: LS-C383557

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA

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