IHC-plus™ MLH1 Antibody (clone 4C9C7) LS-B3475
LifeSpan Biosciences
catalog: LS-B3475

mouse monoclonal (4C9C7)
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Anti-MLH1 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
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MLH1 Antibody (aa387-403, clone 164C819) LS-C566
LifeSpan Biosciences
catalog: LS-C566

mouse monoclonal (164C819)
reactivity: human
application: western blot

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MLH1 Antibody (clone G168-15) LS-C95607
LifeSpan Biosciences
catalog: LS-C95607

mouse monoclonal (G168-15)
reactivity: human
application: immunohistochemistry, immunohistochemistry - paraffin section

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MLH1 Antibody LS-C109333
LifeSpan Biosciences
catalog: LS-C109333

domestic rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry

Immunofluorescence of paraformaldehyde-fixed HeLa, using MLH1 antibody at 1:200 dilution.

Sample (30 ug of whole cell lysate). A: A549. 7.5% SDS PAGE. MLH1 antibody diluted at 1:1000.
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MLH1 Antibody (aa35-84) LS-C111793
LifeSpan Biosciences
catalog: LS-C111793

domestic rabbit polyclonal
reactivity: human
application: western blot

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MLH1 Antibody (aa441-490) LS-C119574
LifeSpan Biosciences
catalog: LS-C119574

domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot analysis of lysates from K562 cells, using MLH1 Antibody. The lane on the right is blocked with the synthesized peptide.
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MLH1 Antibody (clone M1) LS-C133324
LifeSpan Biosciences
catalog: LS-C133324

mouse monoclonal (M1)
reactivity: human
application: western blot, ELISA, immunocytochemistry

Western blot of MLH1 expression in HeLa nuclear extract.

Immunofluorescence of monoclonal antibody to MLH1 on HeLa cell. [antibody concentration 10 ug/ml]

Detection limit for recombinant GST tagged MLH1 is approximately 0.03 ng/ml as a capture antibody.
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MLH1 Antibody (aa458-489) LS-C158152
LifeSpan Biosciences
catalog: LS-C158152

mouse monoclonal
reactivity: human
application: western blot

MLH1 Antibody (Ascites)western blot of A549 cell line lysates (35 ug/lane). The MLH1 antibody detected the MLH1 protein (arrow).
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MLH1 Antibody (aa452-480) LS-C164215
LifeSpan Biosciences
catalog: LS-C164215

domestic rabbit polyclonal
reactivity: human
application: western blot, flow cytometry

MLH1 Antibody western blot of K562 cell line lysates (35 ug/lane). The MLH1 antibody detected the MLH1 protein (arrow).

MLH1 Antibody flow cytometry of K562 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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MLH1 Antibody (Ser467) LS-C176980
LifeSpan Biosciences
catalog: LS-C176980

domestic rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry

Western blot of MLH1 (S467) pAb in extracts from K562 cells.
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MLH1 Antibody (aa452-480, APC) LS-C263228
LifeSpan Biosciences
catalog: LS-C263228

domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA, flow cytometry

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MLH1 Antibody (aa452-480, Biotin) LS-C263229
LifeSpan Biosciences
catalog: LS-C263229

domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA, flow cytometry

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MLH1 Antibody (aa452-480, AP) LS-C263230
LifeSpan Biosciences
catalog: LS-C263230

domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA, flow cytometry

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MLH1 Antibody (aa452-480, FITC) LS-C263231
LifeSpan Biosciences
catalog: LS-C263231

domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA, flow cytometry

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MLH1 Antibody (aa452-480, Azide-free, HRP) LS-C263234
LifeSpan Biosciences
catalog: LS-C263234

domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA, flow cytometry

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MLH1 Antibody (aa452-480, PE) LS-C263235
LifeSpan Biosciences
catalog: LS-C263235

domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA, flow cytometry

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MLH1 Antibody (aa700-C-term) LS-C286959
LifeSpan Biosciences
catalog: LS-C286959

domestic rabbit polyclonal
reactivity: human
application: western blot, immunoprecipitation

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MLH1 Antibody (aa466-496) LS-C164216
LifeSpan Biosciences
catalog: LS-C164216

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry

Western blot analysis of MLH1 (arrow) using rabbit polyclonal MLH1 Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MLH1 gene (Lane 2).

Formalin-fixed and paraffin-embedded human prostate carcinoma reacted with MLH1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

MLH1 Antibody (Center) flow cytometry analysis of 293 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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MLH1 Antibody (clone G168-728) LS-C312102
LifeSpan Biosciences
catalog: LS-C312102

mouse monoclonal (G168-728)
reactivity: human
application: immunohistochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

IHC of MLH1 on FFPE Colon Carcinoma tissue.
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MLH1 Antibody (aa452-480) LS-C323025
LifeSpan Biosciences
catalog: LS-C323025

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, flow cytometry

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MLH1 Antibody LS-C330874
LifeSpan Biosciences
catalog: LS-C330874

domestic rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry

Western blot analysis of extracts of various cell lines, using MLH1 antibody.

Immunofluorescence analysis of U2OS cells using MLH1 antibody. Blue: DAPI for nuclear staining.
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MLH1 Antibody (aa299-527, clone OTI1C1) LS-C339449
LifeSpan Biosciences
catalog: LS-C339449

mouse monoclonal (OTI1C1)
reactivity: human
application: immunohistochemistry, immunohistochemistry - paraffin section

IHC of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-MLH1 mouse monoclonal antibody. (heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
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MLH1 Antibody LS-C342042
LifeSpan Biosciences
catalog: LS-C342042

mouse monoclonal
reactivity: human
application: western blot, immunocytochemistry

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MLH1 Antibody (clone G168-728, Ready-to-Use) LS-C348524
LifeSpan Biosciences
catalog: LS-C348524

mouse monoclonal (G168-728)
reactivity: human
application: immunohistochemistry - paraffin section, immunohistochemistry - frozen section

IHC of MLH1 on FFPE Colon Carcinoma tissue. This image was taken for the unmodified form of this product. Other forms have not been tested.
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MLH1 Antibody (Internal) LS-C352510
LifeSpan Biosciences
catalog: LS-C352510

domestic rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry

Western blot analysis of MLH1 expression in KNRK (A); HeLa (B); A431 (C); SW480 (D) whole cell lysates.

Immunofluorescent analysis of MLH1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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MLH1 Antibody (410-490 aa, Internal) LS-C384568
LifeSpan Biosciences
catalog: LS-C384568

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunocytochemistry

Western blot of MLH1 antibody
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