MCM2 Antibody (clone CRCT2.1) LS-C11595
LifeSpan Biosciences
catalog: LS-C11595

mouse monoclonal (CRCT2.1)
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

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PathPlus™ MCM2 Antibody (aa15-40) LS-B391
LifeSpan Biosciences
catalog: LS-B391

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section

Anti-MCM2 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
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MCM2 Antibody (aa28-77) LS-C31454
LifeSpan Biosciences
catalog: LS-C31454

domestic rabbit polyclonal
reactivity: human
application: western blot

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MCM2 Antibody LS-C48681
LifeSpan Biosciences
catalog: LS-C48681

domestic rabbit polyclonal
reactivity: human
application: western blot, immunoprecipitation

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MCM2 Antibody LS-C72268
LifeSpan Biosciences
catalog: LS-C72268

mouse monoclonal
reactivity: human
application: western blot

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MCM2 Antibody (clone CRCT2.1) LS-C87828
LifeSpan Biosciences
catalog: LS-C87828

mouse monoclonal (CRCT2.1)
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Formalin-fixed, paraffin-embedded human tonsil stained with peroxidase-conjugate and AEC chromogen. Note nuclear staining of lymphocytes. This image was taken for the unmodified form of this product. Other forms have not been tested.
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MCM2 Antibody (clone CRCT2.1, Azide-free) LS-C87829
LifeSpan Biosciences
catalog: LS-C87829

mouse monoclonal (CRCT2.1)
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Formalin-fixed, paraffin-embedded human tonsil stained with peroxidase-conjugate and AEC chromogen. Note nuclear staining of lymphocytes. This image was taken for the unmodified form of this product. Other forms have not been tested.
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MCM2 Antibody (aa828-877) LS-C110186
LifeSpan Biosciences
catalog: LS-C110186

domestic rabbit polyclonal
reactivity: human
application: western blot

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MCM2 Antibody (aa143-296) LS-C125922
LifeSpan Biosciences
catalog: LS-C125922

domestic goat polyclonal
reactivity: human
application: western blot

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PathPlus™ MCM2 Antibody (aa1-50) LS-B7044
LifeSpan Biosciences
catalog: LS-B7044

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Anti-MCM2 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.

Immunofluorescence analysis of HepG2 cells, using MCM2 Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from 293 cells, using MCM2 Antibody. The lane on the right is blocked with the synthesized peptide.
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MCM2 Antibody (aa713-742) LS-C158232
LifeSpan Biosciences
catalog: LS-C158232

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot

Mouse Mcm2 Antibody western blot of mouse Neuro-2a cell line lysates (35 ug/lane). The Mcm2 antibody detected the Mcm2 protein (arrow).
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MCM2 Antibody (aa649-677) LS-C164090
LifeSpan Biosciences
catalog: LS-C164090

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Western blot of MCM2 Antibody in HL-60 cell line lysates (35 ug/lane). MCM2 (arrow) was detected using the purified antibody.

MCM2 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human colon carcinoma followed by peroxidase-conjugated secondary antibody and DAB staining.
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MCM2 Antibody (clone 1E7) LS-C169438
LifeSpan Biosciences
catalog: LS-C169438

mouse monoclonal (1E7)
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section

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MCM2 Antibody (Ala11) LS-C176112
LifeSpan Biosciences
catalog: LS-C176112

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot of MCM2 (A11) pAb in extracts from 293 cells.

IHC of MCM2 (A11) pAb in paraffin-embedded human breast carcinoma tissue.
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MCM2 Antibody (clone 4B8) LS-C179800
LifeSpan Biosciences
catalog: LS-C179800

mouse monoclonal (4B8)
reactivity: human
application: western blot, immunocytochemistry

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MCM2 Antibody (clone 2546C1a) LS-C184630
LifeSpan Biosciences
catalog: LS-C184630

mouse monoclonal (2546C1a)
reactivity: human
application: western blot, immunocytochemistry

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MCM2 Antibody (clone 6A8) LS-C197660
LifeSpan Biosciences
catalog: LS-C197660

mouse monoclonal (6A8)
reactivity: human
application: western blot, ELISA, immunocytochemistry, immunoprecipitation

Western Blot analysis of MCM2 expression in transfected 293T cell line by MCM2 monoclonal antibody (M01), clone 6A8.Lane 1: MCM2 transfected lysate(101.9 KDa).Lane 2: Non-transfected lysate.

Immunofluorescence of monoclonal antibody to MCM2 on HeLa cell. [antibody concentration 10 ug/ml]

Immunoprecipitation of MCM2 transfected lysate using anti-MCM2 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with MCM2 rabbit polyclonal antibody.
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MCM2 Antibody (clone CRCT2.1, Concentrated) LS-C202580
LifeSpan Biosciences
catalog: LS-C202580

mouse monoclonal (CRCT2.1)
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

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MCM2 Antibody (aa649-677, AP) LS-C223234
LifeSpan Biosciences
catalog: LS-C223234

domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA, immunohistochemistry

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MCM2 Antibody (aa649-677, APC) LS-C223235
LifeSpan Biosciences
catalog: LS-C223235

domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA, immunohistochemistry

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MCM2 Antibody (aa649-677, Biotin) LS-C223236
LifeSpan Biosciences
catalog: LS-C223236

domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA, immunohistochemistry

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MCM2 Antibody (aa649-677, FITC) LS-C223237
LifeSpan Biosciences
catalog: LS-C223237

domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA, immunohistochemistry

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MCM2 Antibody (aa649-677, Azide-free, HRP) LS-C223238
LifeSpan Biosciences
catalog: LS-C223238

domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA, immunohistochemistry

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MCM2 Antibody (aa649-677, PE) LS-C223239
LifeSpan Biosciences
catalog: LS-C223239

domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA, immunohistochemistry

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MCM2 Antibody (aa713-742, APC) LS-C223240
LifeSpan Biosciences
catalog: LS-C223240

domestic rabbit polyclonal
reactivity: human, mouse
conjugate: APC
application: western blot, ELISA

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MCM2 Antibody (aa713-742, AP) LS-C223241
LifeSpan Biosciences
catalog: LS-C223241

domestic rabbit polyclonal
reactivity: human, mouse
conjugate: AP
application: western blot, ELISA

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MCM2 Antibody (aa713-742, Biotin) LS-C223242
LifeSpan Biosciences
catalog: LS-C223242

domestic rabbit polyclonal
reactivity: human, mouse
conjugate: biotin
application: western blot, ELISA

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MCM2 Antibody (aa713-742, FITC) LS-C223243
LifeSpan Biosciences
catalog: LS-C223243

domestic rabbit polyclonal
reactivity: human, mouse
conjugate: FITC
application: western blot, ELISA

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MCM2 Antibody (aa713-742, Azide-free, HRP) LS-C223245
LifeSpan Biosciences
catalog: LS-C223245

domestic rabbit polyclonal
reactivity: human, mouse
conjugate: HRP
application: western blot, ELISA

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MCM2 Antibody (aa713-742, PE) LS-C223247
LifeSpan Biosciences
catalog: LS-C223247

domestic rabbit polyclonal
reactivity: human, mouse
conjugate: PE
application: western blot, ELISA

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IHC-plus™ MCM2 Antibody (clone 2B3) LS-B10738
LifeSpan Biosciences
catalog: LS-B10738

mouse monoclonal (2B3)
reactivity: human
application: western blot, ELISA, immunohistochemistry, flow cytometry, immunohistochemistry - paraffin section

Anti-MCM2 antibody IHC staining of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
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MCM2 Antibody (phospho-Ser108) LS-C285943
LifeSpan Biosciences
catalog: LS-C285943

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: immunohistochemistry, immunocytochemistry

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MCM2 Antibody (phospho-Ser41) LS-C285953
LifeSpan Biosciences
catalog: LS-C285953

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: immunohistochemistry

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MCM2 Antibody (phospho-Ser53) LS-C286342
LifeSpan Biosciences
catalog: LS-C286342

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: immunohistochemistry

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MCM2 Antibody (phospho-Ser40/41) LS-C286354
LifeSpan Biosciences
catalog: LS-C286354

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: immunohistochemistry

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MCM2 Antibody (phospho-Ser108) LS-C287032
LifeSpan Biosciences
catalog: LS-C287032

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry

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MCM2 Antibody (phospho-Ser41) LS-C287052
LifeSpan Biosciences
catalog: LS-C287052

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry

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MCM2 Antibody (phospho-Ser53) LS-C287626
LifeSpan Biosciences
catalog: LS-C287626

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry

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MCM2 Antibody (phospho-Ser40/41) LS-C287647
LifeSpan Biosciences
catalog: LS-C287647

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry, flow cytometry

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MCM2 Antibody LS-C310967
LifeSpan Biosciences
catalog: LS-C310967

domestic rabbit polyclonal
reactivity: human
application: western blot, immunoprecipitation

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MCM2 Antibody LS-C310969
LifeSpan Biosciences
catalog: LS-C310969

domestic rabbit polyclonal
reactivity: human
application: western blot

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MCM2 Antibody (clone RBT-MCM2) LS-C312097
LifeSpan Biosciences
catalog: LS-C312097

domestic rabbit recombinant (RBT-MCM2)
reactivity: human
application: immunohistochemistry - paraffin section, immunohistochemistry - frozen section

IHC of MCM-2 on FFPE HSIL of the Cervix.
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MCM2 Antibody (aa889-904) LS-C312810
LifeSpan Biosciences
catalog: LS-C312810

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

WB of MCM2 antibody. All lanes: Anti-MCM2 at 0.5ug/ml. Lane 1: SW620 Whole Cell Lysate at 40ug. Lane 2: PANC Whole Cell Lysate at 40ug. Lane 3: JURKAT Whole Cell Lysate at 40ug. Lane 4: HELA Whole Cell Lysate at 40ug. Predicted bind size: 102KD. Observed bind size: 102KD.

MCM2 antibody. ICC: A549 Cell.
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MCM2 Antibody (aa884-904) LS-C312866
LifeSpan Biosciences
catalog: LS-C312866

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

MCM2 antibody. IHC(P): Rat Intestinal lymphocyte Tissue.

WB of MCM2 antibody. Lane 1: Rat Testis Tissue Lysate. Lane 2: Rat Kidney Tissue Lysate. Lane 3: Mouse Intestine Tissue Lysate. Lane 4: Mouse Testis Tissue Lysate. Lane 5: Mouse Ovary Tissue Lysate. Lane 6: Mouse Kidney Tissue Lysate.
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MCM2 Antibody (FITC) LS-C318494
LifeSpan Biosciences
catalog: LS-C318494

domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA

Western blot of anti-human DNA replication licensing factor MCM2 antibody at 2 ug/ml with HeLa whole cell lysate lane 1: Jurkat whole cell lysate lane 2: K562 whole cell lysate lane 3: MCF-7 whole cell lysate. Secondary: Goat polyclonal to Rabbit IgG at 1:15000 dilution. Predicted band size: 102 KDa. Observed band size: 102 KDa. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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MCM2 Antibody (Biotin) LS-C318496
LifeSpan Biosciences
catalog: LS-C318496

domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA

Western blot of anti-human DNA replication licensing factor MCM2 antibody at 2 ug/ml with HeLa whole cell lysate lane 1: Jurkat whole cell lysate lane 2: K562 whole cell lysate lane 3: MCF-7 whole cell lysate. Secondary: Goat polyclonal to Rabbit IgG at 1:15000 dilution. Predicted band size: 102 KDa. Observed band size: 102 KDa. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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MCM2 Antibody (HRP) LS-C318498
LifeSpan Biosciences
catalog: LS-C318498

domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA

Western blot of anti-human DNA replication licensing factor MCM2 antibody at 2 ug/ml with HeLa whole cell lysate lane 1: Jurkat whole cell lysate lane 2: K562 whole cell lysate lane 3: MCF-7 whole cell lysate. Secondary: Goat polyclonal to Rabbit IgG at 1:15000 dilution. Predicted band size: 102 KDa. Observed band size: 102 KDa. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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MCM2 Antibody LS-C192328
LifeSpan Biosciences
catalog: LS-C192328

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

Western blot of MCM2 pAb in extracts from PC3, K562, 293 and Hela cells.
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MCM2 Antibody (aa713-742) LS-C322911
LifeSpan Biosciences
catalog: LS-C322911

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, ELISA

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MCM2 Antibody (aa649-677) LS-C322915
LifeSpan Biosciences
catalog: LS-C322915

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry

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MCM2 Antibody LS-C330979
LifeSpan Biosciences
catalog: LS-C330979

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

Western blot analysis of extracts of various cell lines, using MCM2 antibody at 1:500 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90s.

Immunohistochemistry of paraffin-embedded mouse spleen using MCM2 antibody(40x lens).
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MCM2 Antibody LS-C331229
LifeSpan Biosciences
catalog: LS-C331229

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

Western blot analysis of extracts of various cell lines.

Immunohistochemistry of paraffin-embedded human colon using MCM2 antibodyat dilution of 1:200 (40x lens).
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MCM2 Antibody (clone OTI1G4) LS-C339608
LifeSpan Biosciences
catalog: LS-C339608

mouse monoclonal (OTI1G4)
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MCM2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MCM2.

IHC of paraffin-embedded Human colon tissue using anti-MCM2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
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MCM2 Antibody (clone OTI3C12) LS-C339609
LifeSpan Biosciences
catalog: LS-C339609

mouse monoclonal (OTI3C12)
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MCM2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MCM2.

IHC of paraffin-embedded Human colon tissue using anti-MCM2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
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MCM2 Antibody (clone OTI1H2) LS-C339616
LifeSpan Biosciences
catalog: LS-C339616

mouse monoclonal (OTI1H2)
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MCM2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MCM2.

IHC of paraffin-embedded Human tonsil using anti-MCM2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
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MCM2 Antibody (clone OTI3B12) LS-C339621
LifeSpan Biosciences
catalog: LS-C339621

mouse monoclonal (OTI3B12)
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MCM2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MCM2.

IHC of paraffin-embedded Human colon tissue using anti-MCM2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
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MCM2 Antibody (clone OTI1G10) LS-C339623
LifeSpan Biosciences
catalog: LS-C339623

mouse monoclonal (OTI1G10)
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MCM2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MCM2.

IHC of paraffin-embedded Human colon tissue using anti-MCM2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
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MCM2 Antibody (clone OTI4C9) LS-C339637
LifeSpan Biosciences
catalog: LS-C339637

mouse monoclonal (OTI4C9)
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MCM2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MCM2.

IHC of paraffin-embedded Human colon tissue using anti-MCM2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
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MCM2 Antibody (clone OTI8A11) LS-C339643
LifeSpan Biosciences
catalog: LS-C339643

mouse monoclonal (OTI8A11)
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MCM2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MCM2.

IHC of paraffin-embedded Human colon tissue using anti-MCM2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
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MCM2 Antibody (clone OTI3B6) LS-C339670
LifeSpan Biosciences
catalog: LS-C339670

mouse monoclonal (OTI3B6)
reactivity: human
application: western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MCM2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MCM2.
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MCM2 Antibody (clone OTI1C7) LS-C339671
LifeSpan Biosciences
catalog: LS-C339671

mouse monoclonal (OTI1C7)
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MCM2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MCM2.

IHC of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-MCM2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
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MCM2 Antibody (clone OTI1B1) LS-C339724
LifeSpan Biosciences
catalog: LS-C339724

mouse monoclonal (OTI1B1)
reactivity: human
application: immunohistochemistry, immunohistochemistry - paraffin section

IHC of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-MCM2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
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MCM2 Antibody (clone RBT-MCM2, Ready-to-Use) LS-C348521
LifeSpan Biosciences
catalog: LS-C348521

domestic rabbit recombinant (RBT-MCM2)
reactivity: human
application: immunohistochemistry - paraffin section, immunohistochemistry - frozen section

IHC of MCM-2 on FFPE HSIL of the Cervix. This image was taken for the unmodified form of this product. Other forms have not been tested.
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MCM2 Antibody (N-Terminus) LS-C352500
LifeSpan Biosciences
catalog: LS-C352500

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot analysis of MCM2 expression in HeLa (A); Jurkat (B); HeLa (C) whole cell lysates.

Immunohistochemical analysis of MCM2 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Immunofluorescent analysis of MCM2 staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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MCM2 Antibody LS-C356942
LifeSpan Biosciences
catalog: LS-C356942

domestic rabbit polyclonal
reactivity: human
application: western blot

All lanes : Anti-MCM2 Antibody at 1:4000 dilution Lane 1: HeLa whole cell lysates Lane 2: MCF-7 whole cell lysates Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution Predicted band size : 102 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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MCM2 Antibody (aa473-679) LS-C372813
LifeSpan Biosciences
catalog: LS-C372813

domestic rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry, immunoprecipitation

Western Blot;Sample: Lane1: Human K562 Cells; Lane2: Human Hela Cells; Lane3: Human Jurkat Cells.

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MCM2 Antibody (1-80 aa, N-terminal) LS-C382294
LifeSpan Biosciences
catalog: LS-C382294

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry

Western blot of BM28 antibody
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