IHC-plus™ SMAD9 Antibody (aa166-179) LS-B2646
LifeSpan Biosciences
catalog: LS-B2646

domestic goat polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section

Antibody (0.3 ug/ml) staining of Human Skin lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-SMAD9 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
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SMAD9 Antibody (aa200-228) LS-C161303
LifeSpan Biosciences
catalog: LS-C161303

domestic rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry
citations: 1

SMAD9 Antibody western blot of A549 cell line lysates (35 ug/lane). The SMAD9 antibody detected the SMAD9 protein (arrow).

Fluorescent confocal image of MCF-7 cell stained with SMAD9 Antibody. MCF-7 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with SMAD9 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). SMAD9 immunoreactivity is localized to cytoplasm and nucleus significantly.
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SMAD9 Antibody (aa18-32) LS-C39136
LifeSpan Biosciences
catalog: LS-C39136

domestic rabbit polyclonal
reactivity: human
application: western blot

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SMAD9 Antibody (aa166-430) LS-C186364
LifeSpan Biosciences
catalog: LS-C186364

domestic rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry

Sample (30 ug of whole cell lysate). A: NT2D1. 10% SDS PAGE. SMAD9 antibody diluted at 1:500.

Immunofluorescence of paraformaldehyde-fixed HeLa using SMAD9 antibody at 1:500 dilution.
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SMAD9 Antibody (aa158-223) LS-C182951
LifeSpan Biosciences
catalog: LS-C182951

domestic sheep polyclonal
reactivity: human
application: immunohistochemistry, immunocytochemistry

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SMAD9 Antibody (clone 3E5) LS-C197638
LifeSpan Biosciences
catalog: LS-C197638

mouse monoclonal (3E5)
reactivity: human
application: western blot, ELISA

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SMAD9 Antibody (aa200-228, AP) LS-C269643
LifeSpan Biosciences
catalog: LS-C269643

domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA

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SMAD9 Antibody (aa200-228, APC) LS-C269644
LifeSpan Biosciences
catalog: LS-C269644

domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA

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SMAD9 Antibody (aa200-228, Biotin) LS-C269646
LifeSpan Biosciences
catalog: LS-C269646

domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA

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SMAD9 Antibody (aa200-228, FITC) LS-C269647
LifeSpan Biosciences
catalog: LS-C269647

domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA

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SMAD9 Antibody (aa200-228, Azide-free, HRP) LS-C269648
LifeSpan Biosciences
catalog: LS-C269648

domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA

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SMAD9 Antibody (aa200-228, PE) LS-C269650
LifeSpan Biosciences
catalog: LS-C269650

domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA

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SMAD9 Antibody (aa226-459) LS-C314902
LifeSpan Biosciences
catalog: LS-C314902

domestic rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry, immunoprecipitation

Western Blot; Lane1: Human Hela Cells; Lane2: Human 293T Cells; Lane3: Human HepG2 Cells.

Human Heart: Formalin-Fixed, Paraffin-Embedded (FFPE)
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SMAD9 Antibody (aa200-228) LS-C325192
LifeSpan Biosciences
catalog: LS-C325192

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA

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SMAD9 Antibody LS-C349210
LifeSpan Biosciences
catalog: LS-C349210

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry

Western blot analysis of extracts of various cell lines.

Immunohistochemistry of paraffin-embedded human liver injury tissue.

Immunofluorescence analysis of A549 cells.
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SMAD9 Antibody (aa1-300) LS-C376625
LifeSpan Biosciences
catalog: LS-C376625

domestic rabbit polyclonal
reactivity: human
application: ELISA, immunohistochemistry, immunohistochemistry - paraffin section

Immunohistochemistry of paraffin-embedded human kidney using antibody at 1:100 dilution.
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SMAD9 Antibody (aa1-300) LS-C376626
LifeSpan Biosciences
catalog: LS-C376626

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section

Western blot. All lanes: Mothers against decapentaplegic homolog 9 antibody at 2 ug/ml+293T whole cell lysate. Secondary antibody: Goat polyclonal to rabbit at 1:10000 dilution. Predicted band size: 52 kDa. Observed band size: 52 kDa Immunohistochemistry.

Immunohistochemistry of paraffin-embedded kidney tonsil using antibody at 1:100 dilution.
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