SMAD3 Antibody (C-Terminus) LS-C18974
LifeSpan Biosciences
catalog: LS-C18974
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section
quantity:
price:
to the supplier
IHC-plus™ SMAD3 Antibody (phospho-Ser423/425) LS-B64
LifeSpan Biosciences
catalog: LS-B64
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, ELISA, immunohistochemistry, flow cytometry, immunohistochemistry - paraffin section
citations: 1

Anti-SMAD3 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
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SMAD3 Antibody LS-C10221
LifeSpan Biosciences
catalog: LS-C10221
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry
quantity:
price:
to the supplier
SMAD3 Antibody (phospho-Ser423/Ser425) LS-C10222
LifeSpan Biosciences
catalog: LS-C10222
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot
quantity:
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to the supplier
SMAD3 Antibody (N-Terminus) LS-C31014
LifeSpan Biosciences
catalog: LS-C31014
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section
quantity:
price:
to the supplier
SMAD3 Antibody (N-Terminus) LS-C39132
LifeSpan Biosciences
catalog: LS-C39132
domestic rabbit monoclonal
reactivity: human
application: western blot, ELISA, immunocytochemistry, immunoprecipitation, flow cytometry
quantity:
price:
to the supplier
SMAD3 Antibody (C-Terminus) LS-C46894
LifeSpan Biosciences
catalog: LS-C46894
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA
quantity:
price:
to the supplier
IHC-plus™ SMAD3 Antibody (aa100-150) LS-B2064
LifeSpan Biosciences
catalog: LS-B2064
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Anti-SMAD3 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
quantity:
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SMAD3 Antibody LS-C60097
LifeSpan Biosciences
catalog: LS-C60097
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA
quantity:
price:
to the supplier
SMAD3 Antibody (Ser425) LS-C89103
LifeSpan Biosciences
catalog: LS-C89103
domestic rabbit polyclonal
reactivity: human
application: immunohistochemistry
quantity:
price:
to the supplier
SMAD3 Antibody (Ser208) LS-C89104
LifeSpan Biosciences
catalog: LS-C89104
domestic rabbit polyclonal
reactivity: human
application: immunohistochemistry
quantity:
price:
to the supplier
SMAD3 Antibody (phospho-Ser208) LS-C96970
LifeSpan Biosciences
catalog: LS-C96970
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: immunocytochemistry

Confocal immunofluorescence of Phospho-SMAD3-S208 Antibody with HeLa cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).

Dot blot of anti-hSMAD3-S208 Phospho-specific antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
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SMAD3 Antibody (phospho-Ser213) LS-C96971
LifeSpan Biosciences
catalog: LS-C96971
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

The anti-Phospho-SMAD3-S213 antibody is used in Western blot to detect Phospho-SMAD3-S213 in Ramos tissue lysate
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SMAD2+3 Antibody (aa2-230) LS-C75854
LifeSpan Biosciences
catalog: LS-C75854
domestic goat polyclonal
reactivity: human
modification: acetylated
application: western blot
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SMAD3 Antibody (aa376-425) LS-C117540
LifeSpan Biosciences
catalog: LS-C117540
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Smad3 Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from 293 cells, treated with UV 5', using Smad3 Antibody. The lane on the right is blocked with the synthesized peptide.
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SMAD3 Antibody (aa145-194) LS-C117930
LifeSpan Biosciences
catalog: LS-C117930
domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot analysis of lysates from HT-29 cells, using Smad3 Antibody. The lane on the right is blocked with the synthesized peptide.
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SMAD3 Antibody (aa179-228) LS-C117932
LifeSpan Biosciences
catalog: LS-C117932
domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot analysis of lysates from HT-29 cells, using Smad3 Antibody. The lane on the right is blocked with the synthesized peptide.
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SMAD3 Antibody (aa417-425) LS-C122429
LifeSpan Biosciences
catalog: LS-C122429
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section
quantity:
price:
to the supplier
SMAD3 Antibody (phospho-Ser425) LS-C124554
LifeSpan Biosciences
catalog: LS-C124554
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: immunohistochemistry
quantity:
price:
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SMAD3 Antibody LS-C124555
LifeSpan Biosciences
catalog: LS-C124555
domestic rabbit polyclonal
reactivity: human
application: immunohistochemistry
quantity:
price:
to the supplier
SMAD3 Antibody LS-C124556
LifeSpan Biosciences
catalog: LS-C124556
domestic rabbit polyclonal
reactivity: human
application: immunohistochemistry
quantity:
price:
to the supplier
SMAD3 Antibody (Ser423/Ser425) LS-C126302
LifeSpan Biosciences
catalog: LS-C126302
domestic rabbit polyclonal
reactivity: human
application: western blot
quantity:
price:
to the supplier
SMAD2+3 Antibody (aa186-273) LS-C132140
LifeSpan Biosciences
catalog: LS-C132140
domestic rabbit monoclonal
reactivity: human
application: western blot
quantity:
price:
to the supplier
IHC-plus™ SMAD3 Antibody (clone 2C12) LS-B5455
LifeSpan Biosciences
catalog: LS-B5455
mouse monoclonal (2C12)
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section

SMAD3 monoclonal antibody clone 2C12. Western blot of SMAD3 expression in human colon.

Detection limit for recombinant GST tagged SMAD3 is approximately 0.03 ng/ml as a capture antibody.

Anti-SMAD3 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
quantity:
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IHC-plus™ SMAD3 Antibody (clone 7F3) LS-B5459
LifeSpan Biosciences
catalog: LS-B5459
mouse monoclonal (7F3)
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section

SMAD3 monoclonal antibody clone 7F3. Western blot of SMAD3 expression in human colon.

Detection limit for recombinant GST tagged SMAD3 is approximately 0.1 ng/ml as a capture antibody.

Anti-SMAD3 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.
quantity:
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SMAD3 Antibody (phospho-Thr179) LS-C153854
LifeSpan Biosciences
catalog: LS-C153854
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, ELISA
quantity:
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to the supplier
SMAD3 Antibody (aa179, Internal) LS-C153867
LifeSpan Biosciences
catalog: LS-C153867
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA
quantity:
price:
to the supplier
SMAD3 Antibody (aa127-156) LS-C161296
LifeSpan Biosciences
catalog: LS-C161296
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

SMAD3 Antibody western blot of CEM cell line lysates (35 ug/lane). The SMAD3 antibody detected the SMAD3 protein (arrow).

Confocal immunofluorescence of SMAD3 Antibody with HeLa cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).

SMAD3 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human heart tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
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SMAD3 Antibody (aa186-215) LS-C161298
LifeSpan Biosciences
catalog: LS-C161298
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section

SMAD3 Antibody (S208) western blot of HepG2 and mouse C2C12 cell line lysates (35 ug/lane). The SMAD3 antibody detected the SMAD3 protein (arrow).

SMAD3-S208 Antibody IHC of formalin-fixed and paraffin-embedded brain tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

Confocal immunofluorescence of SMAD3-S208 Antibody with HeLa cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).
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SMAD3 Antibody (clone 5G11) LS-C169223
LifeSpan Biosciences
catalog: LS-C169223
mouse monoclonal (5G11)
reactivity: human
application: western blot, ELISA, immunocytochemistry
quantity:
price:
to the supplier
SMAD3 Antibody (phospho-Thr179) LS-C175877
LifeSpan Biosciences
catalog: LS-C175877
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot

Western blot of p-Smad3 (T179) pAb in extracts from UV-HeLa cells.
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SMAD3 Antibody (phospho-Ser213) LS-C175878
LifeSpan Biosciences
catalog: LS-C175878
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot

Western blot of p-Smad3 (S213) pAb in extracts from HepG2 (treated with UV) cells.
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SMAD3 Antibody (Met200) LS-C175902
LifeSpan Biosciences
catalog: LS-C175902
domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot of Smad3 (M200) pAb in extracts from HepG2, MCF7, SP20 and PC12 cells.
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SMAD3 Antibody (Pro417) LS-C176302
LifeSpan Biosciences
catalog: LS-C176302
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Western blot of Smad3 (P417) pAb in extracts from 293 cells treated with UV 5'.

IHC of Smad3 (P417) pAb in paraffin-embedded human breast carcinoma tissue.
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SMAD3 Antibody (Pro209) LS-C176639
LifeSpan Biosciences
catalog: LS-C176639
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Western blot of Smad3 (P209) pAb in extracts from HT-29 cells.

IHC of Smad3 (P209) pAb in paraffin-embedded human colon carcinoma tissue.
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SMAD3 Antibody (Asn175) LS-C177435
LifeSpan Biosciences
catalog: LS-C177435
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

Western blot of Smad3 (N175) pAb in extracts from HeLa cell.
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to the supplier
SMAD3 Antibody (Met200) LS-C177436
LifeSpan Biosciences
catalog: LS-C177436
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Western blot of Smad3 (M200) pAb in extracts from HUVEC cells.

IHC of Smad3 (M200) pAb in paraffin-embedded human colon carcinoma tissue.
quantity:
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SMAD3 Antibody (aa1-284) LS-C178987
LifeSpan Biosciences
catalog: LS-C178987
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry
quantity:
price:
to the supplier
SMAD3 Antibody (phospho-Ser204) LS-C191886
LifeSpan Biosciences
catalog: LS-C191886
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot
quantity:
price:
to the supplier
SMAD3 Antibody (Pro209) LS-C191893
LifeSpan Biosciences
catalog: LS-C191893
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot
quantity:
price:
to the supplier
SMAD3 Antibody (phospho-Ser204) LS-C199585
LifeSpan Biosciences
catalog: LS-C199585
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot

Western blot analysis of lysates from NIH/3T3 cells treated with Serum 20% 15', using Smad3 (Phospho-Ser204) Antibody. The lane on the right is blocked with the phospho peptide.
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SMAD3 Antibody (aa 174-223) LS-C199586
LifeSpan Biosciences
catalog: LS-C199586
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using Smad3 (Phospho-Ser208) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from Jurkat cells, using Smad3 (Phospho-Ser208) Antibody. The lane on the right is blocked with the phospho peptide.
quantity:
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SMAD3 Antibody (phospho-Ser213) LS-C199587
LifeSpan Biosciences
catalog: LS-C199587
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot

Western blot analysis of lysates from HT29 cells, using Smad3 (Phospho-Ser213) Antibody. The lane on the right is blocked with the phospho peptide.
quantity:
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to the supplier
SMAD3 Antibody (phospho-Ser425) LS-C199588
LifeSpan Biosciences
catalog: LS-C199588
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Smad3 (Phospho-Ser425) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from NIH/3T3 cells treated with IGF-I 100ng/ml 10', using Smad3 (Phospho-Ser425) Antibody. The lane on the right is blocked with the phospho peptide.
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to the supplier
SMAD3 Antibody (phospho-Thr179) LS-C199589
LifeSpan Biosciences
catalog: LS-C199589
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Immunohistochemistry analysis of paraffin-embedded human heart, using Smad3 (Phospho-Thr179) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from HeLa cells treated with TNF 20ng/ml 2', using Smad3 (Phospho-Thr179) Antibody. The lane on the right is blocked with the phospho peptide.
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to the supplier
SMAD3 Antibody (aa186-215, AP) LS-C217598
LifeSpan Biosciences
catalog: LS-C217598
domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry
quantity:
price:
to the supplier
SMAD3 Antibody (aa186-215, Biotin) LS-C217599
LifeSpan Biosciences
catalog: LS-C217599
domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry
quantity:
price:
to the supplier
SMAD3 Antibody (aa186-215, APC) LS-C217600
LifeSpan Biosciences
catalog: LS-C217600
domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry
quantity:
price:
to the supplier
SMAD3 Antibody (aa186-215, FITC) LS-C217601
LifeSpan Biosciences
catalog: LS-C217601
domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry
quantity:
price:
to the supplier
SMAD3 Antibody (aa186-215, Azide-free, HRP) LS-C217602
LifeSpan Biosciences
catalog: LS-C217602
domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry
quantity:
price:
to the supplier
SMAD3 Antibody (aa186-215, PE) LS-C217603
LifeSpan Biosciences
catalog: LS-C217603
domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry
quantity:
price:
to the supplier
SMAD3 Antibody (aa127-156, AP) LS-C217604
LifeSpan Biosciences
catalog: LS-C217604
domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA, immunohistochemistry, immunocytochemistry
quantity:
price:
to the supplier
SMAD3 Antibody (aa127-156, APC) LS-C217605
LifeSpan Biosciences
catalog: LS-C217605
domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA, immunohistochemistry, immunocytochemistry
quantity:
price:
to the supplier
SMAD3 Antibody (aa127-156, Biotin) LS-C217606
LifeSpan Biosciences
catalog: LS-C217606
domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA, immunohistochemistry, immunocytochemistry
quantity:
price:
to the supplier
SMAD3 Antibody (aa127-156, FITC) LS-C217607
LifeSpan Biosciences
catalog: LS-C217607
domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA, immunohistochemistry, immunocytochemistry
quantity:
price:
to the supplier
SMAD3 Antibody (aa127-156, Azide-free, HRP) LS-C217611
LifeSpan Biosciences
catalog: LS-C217611
domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA, immunohistochemistry, immunocytochemistry
quantity:
price:
to the supplier
SMAD3 Antibody (aa127-156, PE) LS-C217612
LifeSpan Biosciences
catalog: LS-C217612
domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA, immunohistochemistry, immunocytochemistry
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price:
to the supplier
SMAD3 Antibody (phospho-Ser208, AP) LS-C220697
LifeSpan Biosciences
catalog: LS-C220697
domestic rabbit polyclonal
reactivity: human
conjugate: AP
modification: phosphorylated
application: ELISA, immunocytochemistry
quantity:
price:
to the supplier
SMAD3 Antibody (phospho-Ser208, APC) LS-C220698
LifeSpan Biosciences
catalog: LS-C220698
domestic rabbit polyclonal
reactivity: human
conjugate: APC
modification: phosphorylated
application: ELISA, immunocytochemistry
quantity:
price:
to the supplier
SMAD3 Antibody (phospho-Ser208, Biotin) LS-C220699
LifeSpan Biosciences
catalog: LS-C220699
domestic rabbit polyclonal
reactivity: human
conjugate: biotin
modification: phosphorylated
application: ELISA, immunocytochemistry
quantity:
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to the supplier
SMAD3 Antibody (phospho-Ser208, FITC) LS-C220700
LifeSpan Biosciences
catalog: LS-C220700
domestic rabbit polyclonal
reactivity: human
conjugate: FITC
modification: phosphorylated
application: ELISA, immunocytochemistry
quantity:
price:
to the supplier
SMAD3 Antibody (phospho-Ser208, Azide-free, HRP) LS-C220701
LifeSpan Biosciences
catalog: LS-C220701
domestic rabbit polyclonal
reactivity: human
conjugate: HRP
modification: phosphorylated
application: ELISA, immunocytochemistry
quantity:
price:
to the supplier
SMAD3 Antibody (phospho-Ser213, AP) LS-C220702
LifeSpan Biosciences
catalog: LS-C220702
domestic rabbit polyclonal
reactivity: human
conjugate: AP
modification: phosphorylated
application: western blot, ELISA, immunohistochemistry
quantity:
price:
to the supplier
SMAD3 Antibody (phospho-Ser208, PE) LS-C220703
LifeSpan Biosciences
catalog: LS-C220703
domestic rabbit polyclonal
reactivity: human
conjugate: PE
modification: phosphorylated
application: ELISA, immunocytochemistry
quantity:
price:
to the supplier
SMAD3 Antibody (phospho-Ser213, APC) LS-C220704
LifeSpan Biosciences
catalog: LS-C220704
domestic rabbit polyclonal
reactivity: human
conjugate: APC
modification: phosphorylated
application: western blot, ELISA, immunohistochemistry
quantity:
price:
to the supplier
SMAD3 Antibody (phospho-Ser213, Biotin) LS-C220705
LifeSpan Biosciences
catalog: LS-C220705
domestic rabbit polyclonal
reactivity: human
conjugate: biotin
modification: phosphorylated
application: western blot, ELISA, immunohistochemistry
quantity:
price:
to the supplier
SMAD3 Antibody (phospho-Ser213, FITC) LS-C220706
LifeSpan Biosciences
catalog: LS-C220706
domestic rabbit polyclonal
reactivity: human
conjugate: FITC
modification: phosphorylated
application: western blot, ELISA, immunohistochemistry
quantity:
price:
to the supplier
SMAD3 Antibody (phospho-Ser213, Azide-free, HRP) LS-C220709
LifeSpan Biosciences
catalog: LS-C220709
domestic rabbit polyclonal
reactivity: human
conjugate: HRP
modification: phosphorylated
application: western blot, ELISA, immunohistochemistry
quantity:
price:
to the supplier
SMAD3 Antibody (phospho-Ser213, PE) LS-C220710
LifeSpan Biosciences
catalog: LS-C220710
domestic rabbit polyclonal
reactivity: human
conjugate: PE
modification: phosphorylated
application: western blot, ELISA, immunohistochemistry
quantity:
price:
to the supplier
SMAD3 Antibody (phospho-Ser208) LS-C291638
LifeSpan Biosciences
catalog: LS-C291638
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, ELISA, immunohistochemistry

Western blot analysis of lysates from Jurkat cells, using Smad3 (Phospho-Ser208) Antibody.

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Smad3 (Phospho-Ser208) Antibody
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SMAD3 Antibody (aa231-425) LS-C295421
LifeSpan Biosciences
catalog: LS-C295421
domestic rabbit polyclonal
reactivity: human, mouse
application: western blot

Western blot of recombinant SMAD3. This image was taken for the unconjugated form of this product. Other forms have not been tested.

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SMAD3 Antibody (aa231-425) LS-C295430
LifeSpan Biosciences
catalog: LS-C295430
domestic rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry, immunoprecipitation

Western Blot; Sample: Rat Pancreas lysate; Primary Ab: 1µg/ml Rabbit Anti-Human Smad3 Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody

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SMAD3 Antibody (aa231-425, Biotin) LS-C297105
LifeSpan Biosciences
catalog: LS-C297105
domestic rabbit polyclonal
reactivity: human, mouse
conjugate: biotin
application: western blot

Western blot of recombinant SMAD3. This image was taken for the unconjugated form of this product. Other forms have not been tested.

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SMAD3 Antibody (phospho-Ser204) LS-C313639
LifeSpan Biosciences
catalog: LS-C313639
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation
quantity:
price:
to the supplier
SMAD3 Antibody (FITC) LS-C317748
LifeSpan Biosciences
catalog: LS-C317748
domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA

Western blot of Actin, cytoplasmic 1 antibody at 2 ug/ml with PC-3 whole cell lysate. Secondary: Goat polyclonal to Rabbit IgG at 1:10000 dilution. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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SMAD3 Antibody (HRP) LS-C317749
LifeSpan Biosciences
catalog: LS-C317749
domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA

Western blot of Actin, cytoplasmic 1 antibody at 2 ug/ml with PC-3 whole cell lysate. Secondary: Goat polyclonal to Rabbit IgG at 1:10000 dilution. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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SMAD3 Antibody LS-C317750
LifeSpan Biosciences
catalog: LS-C317750
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA

Western blot of Actin, cytoplasmic 1 antibody at 2 ug/ml with PC-3 whole cell lysate. Secondary: Goat polyclonal to Rabbit IgG at 1:10000 dilution. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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SMAD3 Antibody (Biotin) LS-C317757
LifeSpan Biosciences
catalog: LS-C317757
domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA

Western blot of Actin, cytoplasmic 1 antibody at 2 ug/ml with PC-3 whole cell lysate. Secondary: Goat polyclonal to Rabbit IgG at 1:10000 dilution. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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SMAD3 Antibody (aa186-215) LS-C325191
LifeSpan Biosciences
catalog: LS-C325191
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry
quantity:
price:
to the supplier
SMAD3 Antibody (aa127-156) LS-C325193
LifeSpan Biosciences
catalog: LS-C325193
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry
quantity:
price:
to the supplier
SMAD3 Antibody (phospho-Ser208) LS-C328687
LifeSpan Biosciences
catalog: LS-C328687
domestic rabbit polyclonal
reactivity: human
application: ELISA, immunocytochemistry
quantity:
price:
to the supplier
SMAD3 Antibody (phospho-Ser213) LS-C328692
LifeSpan Biosciences
catalog: LS-C328692
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry
quantity:
price:
to the supplier
SMAD3 Antibody (phospho-Ser204) LS-C352492
LifeSpan Biosciences
catalog: LS-C352492
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunocytochemistry, immunoprecipitation

Western blot analysis of SMAD3 (pS204) expression in HeLa UV-treated (A); SP2/0 UV-treated (B); H9C2 UV-treated (C) whole cell lysates.

Immunofluorescent analysis of SMAD3 (pS204) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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SMAD3 Antibody (phospho-Ser425) LS-C354028
LifeSpan Biosciences
catalog: LS-C354028
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Western blot analysis of SMAD3 (pS425) expression in A431 EGF-treated (A); HL60 TGF-treated (B) whole cell lysates.

Immunohistochemical analysis of SMAD3 (pS425) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
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IHC-plus™ SMAD3 Antibody (aa170-219) LS-B12424
LifeSpan Biosciences
catalog: LS-B12424
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Human Breast: Formalin-Fixed, Paraffin-Embedded (FFPE)

Western blot analysis of lysates from HUVEC cells, using Smad3 Antibody. The lane on the right is blocked with the synthesized peptide.
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SMAD3 Antibody (aa186-219) LS-C356459
LifeSpan Biosciences
catalog: LS-C356459
domestic rabbit polyclonal
reactivity: human
application: western blot

Anti-SMAD3 Antibody at 1:1000 dilution + mouse brain lysates Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 48 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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SMAD3 Antibody (aa188-222) LS-C356509
LifeSpan Biosciences
catalog: LS-C356509
domestic rabbit polyclonal
reactivity: human, mouse
application: western blot

All lanes : Anti-Smad3 Antibody at 1:1000 dilution Lane 1: M. brain tissue lysates Lane 2: R. brain tissue lysates Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 48 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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SMAD3 Antibody (aa157-191) LS-C356542
LifeSpan Biosciences
catalog: LS-C356542
domestic rabbit polyclonal
reactivity: human, mouse
application: western blot

Anti-Smad3 Antibody at 1:2000 dilution + rat brain lysates Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 48 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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SMAD3 Antibody (aa193-222) LS-C357590
LifeSpan Biosciences
catalog: LS-C357590
domestic rabbit polyclonal
reactivity: human
application: western blot

SMAD3 antibody Western blot. All lanes: Anti SMAD3 at 0.5 ug/ml. Lane 1: U87 Whole Cell Lysate at 40 ug. Lane 2: Human Placenta Tissue Lysate at 50 ug. Lane 3: PANC Whole Cell Lysate at 40 ug. Lane 4: HELA Whole Cell Lysate at 40 ug. Predicted band size: 48 kD. Observed band size: 56 kD.
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SMAD3 Antibody (Internal) LS-C358326
LifeSpan Biosciences
catalog: LS-C358326
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Western blot analysis of SMAD3 expression in SKOV3 (A); HeLa (B); MCF7 (C) whole cell lysates.

Immunohistochemical analysis of SMAD3 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP-conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
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SMAD3 Antibody (phospho-Thr179) LS-C358327
LifeSpan Biosciences
catalog: LS-C358327
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Western blot analysis of SMAD3 (pT179) expression in HeLa (A) whole cell lysates.

Immunohistochemical analysis of SMAD3 (pT179) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP-conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
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SMAD3 Antibody (C-Terminus) LS-C358328
LifeSpan Biosciences
catalog: LS-C358328
domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot analysis of SMAD3 (pS423) expression in HeLa (A); Raw264.7 (B); H9C2 (C) whole cell lysates.
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SMAD3 Antibody (Internal) LS-C358329
LifeSpan Biosciences
catalog: LS-C358329
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot analysis of SMAD3 expression in HeLa (A) whole cell lysates.

Immunohistochemical analysis of SMAD3 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP-conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Immunofluorescent analysis of SMAD3 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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SMAD3 Antibody (phospho-Ser213) LS-C368536
LifeSpan Biosciences
catalog: LS-C368536
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot analysis of SMAD3 (pS213) expression in A549 (A); Jurkat (B) whole cell lysates.

Immunohistochemical analysis of SMAD3 (pS213) staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Immunofluorescent analysis of SMAD3 (pS213) staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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SMAD3 Antibody (aa1-230) LS-C376653
LifeSpan Biosciences
catalog: LS-C376653
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section

Western blot. All lanes: SMAD3 antibody at 8 ug/ml. Lane 1: Jurkat whole cell lysate. Lane 2: A431 whole cell lysate. Secondary antibody: Goat polyclonal to rabbit at 1:10000 dilution. Predicted band size: 48 kDa. Observed band size: 48 kDa.

Immunohistochemistry of paraffin-embedded human colon cancer using antibody at 1:100 dilution.
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SMAD3 Antibody (150-230 aa) LS-C380968
LifeSpan Biosciences
catalog: LS-C380968
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, ELISA, immunohistochemistry
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SMAD3 Antibody (140-220 aa) LS-C380970
LifeSpan Biosciences
catalog: LS-C380970
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, ELISA, immunohistochemistry, immunocytochemistry

Western blot of Phospho-Smad3 (S204) antibody
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SMAD3 Antibody (350-430 aa) LS-C381199
LifeSpan Biosciences
catalog: LS-C381199
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, ELISA, immunohistochemistry

Western blot of Phospho-Smad3 (S425) antibody
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SMAD3 Antibody (120-200 aa) LS-C381350
LifeSpan Biosciences
catalog: LS-C381350
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, ELISA, immunohistochemistry

Western blot of Phospho-Smad3 (T179) antibody
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SMAD3 Antibody (150-230 aa) LS-C381352
LifeSpan Biosciences
catalog: LS-C381352
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, ELISA, immunohistochemistry

Western blot of Phospho-Smad3 (S208) antibody
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SMAD3 Antibody (120-200 aa) LS-C386109
LifeSpan Biosciences
catalog: LS-C386109
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry

Western blot of Smad3 antibody
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SMAD3 Antibody (140-220 aa) LS-C386111
LifeSpan Biosciences
catalog: LS-C386111
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry

Western blot of Smad3 antibody
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SMAD3 Antibody (150-230 aa) LS-C386116
LifeSpan Biosciences
catalog: LS-C386116
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry
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SMAD3 Antibody (350-430 aa) LS-C386120
LifeSpan Biosciences
catalog: LS-C386120
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry

Western blot of Smad3 antibody
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SMAD3 Antibody LS-C387824
LifeSpan Biosciences
catalog: LS-C387824
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

Immunohistochemistry of Smad3 antibody

Western blot of Smad3 antibody
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SMAD3 Antibody LS-C387826
LifeSpan Biosciences
catalog: LS-C387826
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

Immunohistochemistry of Smad3 antibody

Western blot of Smad3 antibody
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