SMAD2 Antibody (aa82-94) LS-C20211
LifeSpan Biosciences
catalog: LS-C20211

domestic goat polyclonal
reactivity: human
application: western blot

Staining (2 ug/ml) of HeLa lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by using chemiluminescence.
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SMAD2 Antibody (N-Terminus) LS-C31653
LifeSpan Biosciences
catalog: LS-C31653

domestic rabbit polyclonal
reactivity: human
application: western blot

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IHC-plus™ SMAD2 Antibody (aa234-249) LS-B2063
LifeSpan Biosciences
catalog: LS-B2063

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Anti-SMAD2 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
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SMAD2 Antibody (phospho-Ser467) LS-C199582
LifeSpan Biosciences
catalog: LS-C199582

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section
citations: 1

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Smad2 (Phospho-Ser467) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from HepG2 cells treated with PMA 125ng/ml 15', using Smad2 (Phospho-Ser467) Antibody. The lane on the right is blocked with the phospho peptide.
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SMAD2 Antibody (phospho-Ser245/Ser250/Ser255) LS-C10212
LifeSpan Biosciences
catalog: LS-C10212

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot

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SMAD2 Antibody (aa186-273) LS-C10213
LifeSpan Biosciences
catalog: LS-C10213

domestic rabbit polyclonal
reactivity: human
application: western blot

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SMAD2 Antibody (phospho-Ser465/Ser467) LS-C10215
LifeSpan Biosciences
catalog: LS-C10215

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot

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SMAD2 Antibody (MH1 Domain) LS-C10216
LifeSpan Biosciences
catalog: LS-C10216

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA

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SMAD2 Antibody (phospho-Ser465/Ser467) LS-C10217
LifeSpan Biosciences
catalog: LS-C10217

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot

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SMAD2 Antibody LS-C60127
LifeSpan Biosciences
catalog: LS-C60127

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunoprecipitation

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SMAD2 Antibody (phospho-Ser467) LS-C89100
LifeSpan Biosciences
catalog: LS-C89100

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot

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SMAD2 Antibody (Ser118) LS-C100546
LifeSpan Biosciences
catalog: LS-C100546

domestic rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry, flow cytometry

Fluorescent confocal image of SY5Y cells stained with SMAD2 antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated SMAD2 primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Note the highly specific localization of the SMAD2 mainly to the nucleus.

Western blot of SMAD2 Antibody in NIH-3T3 cell line lysates (35 ug/lane). SMAD2 (arrow) was detected using the purified antibody.

SMAD2 Antibody flow cytometry of HeLa cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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SMAD2 Antibody (N-Terminus) LS-C109632
LifeSpan Biosciences
catalog: LS-C109632

domestic rabbit polyclonal
reactivity: human
application: western blot

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SMAD2 Antibody (C-Terminus) LS-C116890
LifeSpan Biosciences
catalog: LS-C116890

domestic rabbit polyclonal
reactivity: human
application: western blot

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SMAD2 Antibody (aa221-270) LS-C117926
LifeSpan Biosciences
catalog: LS-C117926

domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot analysis of lysates from HepG2 cells, using Smad2 Antibody. The lane on the right is blocked with the synthesized peptide.
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SMAD2 Antibody (aa211-260) LS-C117927
LifeSpan Biosciences
catalog: LS-C117927

domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot analysis of lysates from HT-29, NIH/3T3, and HepG2 cells, using Smad2 Antibody. The lane on the right is blocked with the synthesized peptide.
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SMAD2 Antibody (aa186-235) LS-C117928
LifeSpan Biosciences
catalog: LS-C117928

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Immunofluorescence analysis of HeLa cells, using Smad2 Antibody. The picture on the right is blocked with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using Smad2 Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from RAW264.7 and 293 cells, using Smad2 Antibody. The lane on the right is blocked with the synthesized peptide.
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SMAD2 Antibody LS-C132137
LifeSpan Biosciences
catalog: LS-C132137

domestic rabbit polyclonal
reactivity: human
application: western blot

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SMAD2 Antibody (phospho-Ser467) LS-C132138
LifeSpan Biosciences
catalog: LS-C132138

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot

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SMAD2 Antibody (clone 4D10) LS-C133302
LifeSpan Biosciences
catalog: LS-C133302

mouse monoclonal (4D10)
reactivity: human
application: western blot, ELISA, immunocytochemistry

SMAD2 monoclonal antibody (M01), clone 4D10 Western blot of SMAD2 expression in HeLa NE.

Immunofluorescence of monoclonal antibody to SMAD2 on HeLa cell. [antibody concentration 10 ug/ml]

Detection limit for recombinant GST tagged SMAD2 is approximately 1 ng/ml as a capture antibody.
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SMAD2 Antibody (clone 3G9) LS-C133304
LifeSpan Biosciences
catalog: LS-C133304

mouse monoclonal (3G9)
reactivity: human
application: western blot, ELISA, immunocytochemistry

SMAD2 monoclonal antibody (M05), clone 3G9 Western blot of SMAD2 expression in HeLa NE.

Immunofluorescence of monoclonal antibody to SMAD2 on HeLa cell. [antibody concentration 10 ug/ml]

Detection limit for recombinant GST tagged SMAD2 is approximately 0.1 ng/ml as a capture antibody.
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IHC-plus™ SMAD2 Antibody (clone 3G6) LS-B5454
LifeSpan Biosciences
catalog: LS-B5454

mouse monoclonal (3G6)
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section

SMAD2 monoclonal antibody clone 3G6 Western blot of SMAD2 expression in A-431.

Detection limit for recombinant GST tagged SMAD2 is approximately 0.1 ng/ml as a capture antibody.

Anti-SMAD2 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
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SMAD2 Antibody LS-C137206
LifeSpan Biosciences
catalog: LS-C137206

domestic rabbit polyclonal
reactivity: human
application: western blot

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SMAD2 Antibody (clone 5G7) LS-C169392
LifeSpan Biosciences
catalog: LS-C169392

mouse monoclonal (5G7)
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry

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SMAD2 Antibody (clone OTI2C10) LS-C115645
LifeSpan Biosciences
catalog: LS-C115645

mouse monoclonal (OTI2C10)
reactivity: human
application: western blot, immunocytochemistry

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SMAD2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SMAD2.

Anti-SMAD2 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SMAD2.
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SMAD2 Antibody (clone OTI4G7) LS-C115681
LifeSpan Biosciences
catalog: LS-C115681

mouse monoclonal (OTI4G7)
reactivity: human
application: western blot, immunocytochemistry, flow cytometry

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SMAD2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SMAD2.

Anti-SMAD2 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SMAD2.

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SMAD2 antibody, and then analyzed by flow cytometry.
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SMAD2 Antibody (N-Terminus) LS-C171841
LifeSpan Biosciences
catalog: LS-C171841

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

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SMAD2+3 Antibody (N-Terminus, Magnetic beads) LS-C171842
LifeSpan Biosciences
catalog: LS-C171842

domestic rabbit polyclonal
reactivity: human
conjugate: Bead
application: immunoprecipitation

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SMAD2+3 Antibody (N-Terminus, Sepharose) LS-C171843
LifeSpan Biosciences
catalog: LS-C171843

domestic rabbit polyclonal
reactivity: human
application: immunoprecipitation

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SMAD2 Antibody (Ser2) LS-C175900
LifeSpan Biosciences
catalog: LS-C175900

domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot of Smad2 (S2) pAb in extracts from HepG2, A549, PC12 and Raw264.7 cells.
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SMAD2 Antibody (Pro459) LS-C176301
LifeSpan Biosciences
catalog: LS-C176301

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot of Smad2 (P459) pAb in extracts from HepG2 or K562 tested with UV 5'.

IHC of Smad2 (P459) pAb in paraffin-embedded human prostate carcinoma lung carcinoma tissue.
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SMAD2 Antibody (Tyr216) LS-C176615
LifeSpan Biosciences
catalog: LS-C176615

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot of Smad2 (Y216) pAb in extracts from RAW264.7cells.

IHC of Smad2 (Y216) pAb in paraffin-embedded human brain tissue.
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IHC-plus™ SMAD2 Antibody (Thr220) LS-B9109
LifeSpan Biosciences
catalog: LS-B9109

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Fluorescent confocal image of SY5Y cells stained with SMAD2 (T220) antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated SMAD2 (T220) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Note the highly specific localization of the SMAD2 mainly to the nucleus.

Western blot of SMAD2 Antibody (T220) in NCI-H460 cell line lysates (35 ug/lane). SMAD2 (arrow) was detected using the purified antibody.

Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE)
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SMAD2 Antibody (aa216-265) LS-C199579
LifeSpan Biosciences
catalog: LS-C199579

domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot analysis of lysates from COLO205 cells, treated with serum 20% 15', using Smad2 Antibody. The lane on the right is blocked with the synthesized peptide.
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SMAD2 Antibody (phospho-Ser250) LS-C199581
LifeSpan Biosciences
catalog: LS-C199581

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot

Western blot analysis of lysates from 293 cells treated with PMA 125ng/ml 30', using Smad2 (Phospho-Ser250) Antibody. The lane on the right is blocked with the phospho peptide.
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SMAD2 Antibody (phospho-Thr220) LS-C199583
LifeSpan Biosciences
catalog: LS-C199583

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: immunocytochemistry

Immunofluorescence analysis of HeLa cells, using Smad2 (Phospho-Thr220) Antibody. The picture on the right is blocked with the phospho peptide.
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SMAD2 Antibody (aa202-251) LS-C200707
LifeSpan Biosciences
catalog: LS-C200707

domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot analysis of the lysates from HeLa cells using Smad2 antibody.
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SMAD2 Antibody (phospho-Ser118) LS-C203689
LifeSpan Biosciences
catalog: LS-C203689

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: immunocytochemistry

Confocal immunofluorescence of Phospho-SMAD2-S118 Antibody with HeLa cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).

Dot blot of anti-Phospho-SMAD2-S118 Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
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SMAD2 Antibody (phospho-Ser465/467) LS-C209429
LifeSpan Biosciences
catalog: LS-C209429

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, ELISA

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SMAD2 Antibody (phospho-Ser118, APC) LS-C282756
LifeSpan Biosciences
catalog: LS-C282756

domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: ELISA

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SMAD2 Antibody (phospho-Ser118, AP) LS-C282757
LifeSpan Biosciences
catalog: LS-C282757

domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: ELISA

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SMAD2 Antibody (phospho-Ser118, Biotin) LS-C282758
LifeSpan Biosciences
catalog: LS-C282758

domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: ELISA

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SMAD2 Antibody (phospho-Ser118, FITC) LS-C282759
LifeSpan Biosciences
catalog: LS-C282759

domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: ELISA

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SMAD2 Antibody (phospho-Ser118, Azide-free, HRP) LS-C282760
LifeSpan Biosciences
catalog: LS-C282760

domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: ELISA

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SMAD2 Antibody (phospho-Ser118, PE) LS-C282761
LifeSpan Biosciences
catalog: LS-C282761

domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: ELISA

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SMAD2 Antibody (Ser118, APC) LS-C282762
LifeSpan Biosciences
catalog: LS-C282762

domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA

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SMAD2 Antibody (Ser118, Biotin) LS-C282763
LifeSpan Biosciences
catalog: LS-C282763

domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA

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SMAD2 Antibody (Ser118, FITC) LS-C282764
LifeSpan Biosciences
catalog: LS-C282764

domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA

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SMAD2 Antibody (Ser118, AP) LS-C282765
LifeSpan Biosciences
catalog: LS-C282765

domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA

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SMAD2 Antibody (Ser118, Azide-free, HRP) LS-C282766
LifeSpan Biosciences
catalog: LS-C282766

domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA

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SMAD2 Antibody (Thr220, AP) LS-C282767
LifeSpan Biosciences
catalog: LS-C282767

domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA

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SMAD2 Antibody (Ser118, PE) LS-C282768
LifeSpan Biosciences
catalog: LS-C282768

domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA

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SMAD2 Antibody (Thr220, APC) LS-C282769
LifeSpan Biosciences
catalog: LS-C282769

domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA

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SMAD2 Antibody (Thr220, Biotin) LS-C282770
LifeSpan Biosciences
catalog: LS-C282770

domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA

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SMAD2 Antibody (Thr220, FITC) LS-C282773
LifeSpan Biosciences
catalog: LS-C282773

domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA

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SMAD2 Antibody (Thr220, Azide-free, HRP) LS-C282774
LifeSpan Biosciences
catalog: LS-C282774

domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA

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SMAD2 Antibody (Thr220, PE) LS-C282775
LifeSpan Biosciences
catalog: LS-C282775

domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA

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IHC-plus™ SMAD2 Antibody (aa418-467) LS-B10160
LifeSpan Biosciences
catalog: LS-B10160

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Immunofluorescence of HepG2 cells, using Smad2 Antibody. The sample on the right was incubated with synthetic peptide.

Anti-SMAD2 antibody IHC staining of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.

Immunofluorescence analysis of HepG2 cells, using Smad2 Antibody. The picture on the right is blocked with the synthesized peptide.
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SMAD2 Antibody (aa272-467) LS-C295418
LifeSpan Biosciences
catalog: LS-C295418

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot

Western blot of recombinant SMAD2. This image was taken for the unconjugated form of this product. Other forms have not been tested.

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SMAD2 Antibody (aa273-467) LS-C295419
LifeSpan Biosciences
catalog: LS-C295419

domestic rabbit polyclonal
reactivity: human
application: western blot

Western Blot; Sample: Recombinant protein.

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SMAD2 Antibody (aa272-467, Biotin) LS-C298139
LifeSpan Biosciences
catalog: LS-C298139

domestic rabbit polyclonal
reactivity: human, mouse
conjugate: biotin
application: western blot

Western blot of recombinant SMAD2. This image was taken for the unconjugated form of this product. Other forms have not been tested.

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SMAD2 Antibody (aa273-467, Biotin) LS-C301238
LifeSpan Biosciences
catalog: LS-C301238

domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot

Western Blot; Sample: Recombinant protein.

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SMAD2 Antibody (aa272-467, FITC) LS-C302664
LifeSpan Biosciences
catalog: LS-C302664

domestic rabbit polyclonal
reactivity: human, mouse
conjugate: FITC
application: western blot

Western blot of recombinant SMAD2. This image was taken for the unconjugated form of this product. Other forms have not been tested.

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SMAD2 Antibody (aa94-112) LS-C313039
LifeSpan Biosciences
catalog: LS-C313039

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

WB of SMAD2 antibody. Recombinant Protein Detection Source:. E.coli derived -recombinant human SHC1, 35.0KD. (162aa tag+D424-P578). Lane 1: Recombinant Human SHC1 Proteins 10ng. Lane 2: Recombinant Human SHC1 Proteins 5ng. Lane 3: Recombinant Human SHC1 Proteins 2.5ng.

SMAD2 antibody. IHC(P): Human Breast Cancer Tissue.

SMAD2 antibody. ICC: HELA Cell.
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SMAD2 Antibody (Thr220) LS-C328685
LifeSpan Biosciences
catalog: LS-C328685

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA

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SMAD2 Antibody (phospho-Ser118) LS-C328686
LifeSpan Biosciences
catalog: LS-C328686

domestic rabbit polyclonal
reactivity: human
application: ELISA

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SMAD2 Antibody (Ser118) LS-C328688
LifeSpan Biosciences
catalog: LS-C328688

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA

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SMAD2 Antibody LS-C330963
LifeSpan Biosciences
catalog: LS-C330963

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

Western blot analysis of extracts of HeLa cells, using SMAD2 antibody. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.
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SMAD2 Antibody (phospho-Ser467) LS-C335925
LifeSpan Biosciences
catalog: LS-C335925

domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot analysis of extracts of Untreated and treated 293T cell lines.
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SMAD2 Antibody (phospho-Thr220) LS-C335928
LifeSpan Biosciences
catalog: LS-C335928

domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot analysis of extracts from Hela cells.
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SMAD2 Antibody LS-C342059
LifeSpan Biosciences
catalog: LS-C342059

mouse monoclonal
reactivity: human
application: western blot

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SMAD2 Antibody (aa198-232) LS-C344679
LifeSpan Biosciences
catalog: LS-C344679

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot

Western blot of lysates from HeLa, mouse NIH/3T3, mouse C2C12, rat PC-12 cell line (from left to right) with Smad2 Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20 ug per lane.
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SMAD2 Antibody (Internal) LS-C352490
LifeSpan Biosciences
catalog: LS-C352490

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot analysis of SMAD2 expression in A431 (A); Jurkat (B); RAW264.7 (C); PC12 (D) whole cell lysates.

Immunohistochemical analysis of SMAD2 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Immunofluorescent analysis of SMAD2 staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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SMAD2 Antibody (aa106-140) LS-C356519
LifeSpan Biosciences
catalog: LS-C356519

domestic rabbit polyclonal
reactivity: human
application: western blot

Anti-SMAD2 Antibody at 1:1000 dilution + NIH/3T3 whole cell lysates Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 52 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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SMAD2 Antibody (C-Terminus) LS-C358324
LifeSpan Biosciences
catalog: LS-C358324

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Western blot analysis of SMAD2 (pS465) expression in HepG2 (A) whole cell lysates.

Immunohistochemical analysis of SMAD2 (pS465) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP-conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
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SMAD2 Antibody (phospho-Ser467) LS-C358325
LifeSpan Biosciences
catalog: LS-C358325

domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot analysis of SMAD2 (pS467) expression in human kidney (A); mouse brain (B); rat brain (C) whole cell lysates.

Immunohistochemical analysis of SMAD2 (pS467) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP-conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Immunofluorescent analysis of SMAD2 (pS467) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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SMAD2 Antibody (190-270 aa) LS-C380966
LifeSpan Biosciences
catalog: LS-C380966

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, ELISA

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price:
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SMAD2 Antibody (410-490 aa) LS-C381191
LifeSpan Biosciences
catalog: LS-C381191

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, ELISA, immunohistochemistry

Western blot of Phospho-Smad2 (S467) antibody
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SMAD2 Antibody (400-480 aa) LS-C381443
LifeSpan Biosciences
catalog: LS-C381443

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, ELISA, immunohistochemistry

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SMAD2 Antibody (160-240 aa) LS-C381775
LifeSpan Biosciences
catalog: LS-C381775

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: ELISA, immunohistochemistry, immunocytochemistry

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price:
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SMAD2 Antibody (180-260 aa) LS-C386100
LifeSpan Biosciences
catalog: LS-C386100

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA

Western blot of Smad2 antibody
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SMAD2 Antibody (190-270 aa) LS-C386103
LifeSpan Biosciences
catalog: LS-C386103

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA

Western blot of Smad2 antibody
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SMAD2 Antibody (400-480 aa) LS-C386104
LifeSpan Biosciences
catalog: LS-C386104

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA

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price:
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SMAD2 Antibody (160-240 aa) LS-C386105
LifeSpan Biosciences
catalog: LS-C386105

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry

Western blot of Smad2 antibody
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price:
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SMAD2 Antibody (190-270 aa) LS-C386106
LifeSpan Biosciences
catalog: LS-C386106

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry

Western blot of Smad2 antibody
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SMAD2 Antibody (410-490 aa) LS-C386108
LifeSpan Biosciences
catalog: LS-C386108

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry

Western blot of Smad2 antibody
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price:
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SMAD2 Antibody LS-C387861
LifeSpan Biosciences
catalog: LS-C387861

domestic rabbit polyclonal
reactivity: human
application: western blot

quantity:
price:
to the supplier