LIG4 / DNA Ligase IV Antibody (aa275-324) LS-C31455
LifeSpan Biosciences
catalog: LS-C31455

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

quantity:
price:
to the supplier

LIG4 / DNA Ligase IV Antibody (aa591-640) LS-C119547
LifeSpan Biosciences
catalog: LS-C119547

domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot analysis of lysates from Jurkat cells, using DNL4 Antibody. The lane on the right is blocked with the synthesized peptide.
quantity:
price:
to the supplier

LIG4 / DNA Ligase IV Antibody (Gly619) LS-C176883
LifeSpan Biosciences
catalog: LS-C176883

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry

Western blot of DNA Ligase IV (G619) pAb in extracts from Jurkat cells.
quantity:
price:
to the supplier

LIG4 / DNA Ligase IV Antibody (clone 1A4) LS-C197616
LifeSpan Biosciences
catalog: LS-C197616

mouse monoclonal (1A4)
reactivity: human
application: ELISA

quantity:
price:
to the supplier

LIG4 / DNA Ligase IV Antibody (aa239-267) LS-C203791
LifeSpan Biosciences
catalog: LS-C203791

domestic rabbit polyclonal
reactivity: human
application: western blot

LIG4 Antibody western blot of human placenta tissue lysates (35 ug/lane). The LIG4 antibody detected the LIG4 protein (arrow).
quantity:
price:
to the supplier

LIG4 / DNA Ligase IV Antibody (phospho-Thr650) LS-C291176
LifeSpan Biosciences
catalog: LS-C291176

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: immunohistochemistry

quantity:
price:
to the supplier

LIG4 / DNA Ligase IV Antibody (aa616-665) LS-C291228
LifeSpan Biosciences
catalog: LS-C291228

domestic rabbit polyclonal
reactivity: human
application: immunohistochemistry

quantity:
price:
to the supplier

LIG4 / DNA Ligase IV Antibody LS-C331665
LifeSpan Biosciences
catalog: LS-C331665

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

Western blot analysis of extracts of various cell lines, using LIG4 antibody at 1:500 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.
quantity:
price:
to the supplier

LIG4 / DNA Ligase IV Antibody (Internal) LS-C352452
LifeSpan Biosciences
catalog: LS-C352452

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot analysis of DNA Ligase 4 expression in HepG2 (A) whole cell lysates.

Immunohistochemical analysis of DNA Ligase 4 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Immunofluorescent analysis of DNA Ligase 4 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
quantity:
price:
to the supplier

LIG4 / DNA Ligase IV Antibody (590-670 aa) LS-C381605
LifeSpan Biosciences
catalog: LS-C381605

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: ELISA, immunohistochemistry

quantity:
price:
to the supplier

LIG4 / DNA Ligase IV Antibody (590-670 aa) LS-C383149
LifeSpan Biosciences
catalog: LS-C383149

domestic rabbit polyclonal
reactivity: human
application: ELISA, immunohistochemistry

quantity:
price:
to the supplier

LIG4 / DNA Ligase IV Antibody (560-640 aa, Internal) LS-C383151
LifeSpan Biosciences
catalog: LS-C383151

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry

Western blot of DNA Ligase IV antibody
quantity:
price:
to the supplier