ARHGDIA / RHOGDI Antibody (aa140-189) LS-C118020
LifeSpan Biosciences
catalog: LS-C118020

domestic rabbit polyclonal
reactivity: human
application: immunohistochemistry

Immunohistochemistry analysis of paraffin-embedded human cervix carcinoma tissue, using ARHGDIA Antibody. The picture on the right is blocked with the synthesized peptide.
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ARHGDIA / RHOGDI Antibody (C-Terminus) LS-C137057
LifeSpan Biosciences
catalog: LS-C137057

domestic rabbit polyclonal
reactivity: human
application: western blot

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ARHGDIA / RHOGDI Antibody LS-C137481
LifeSpan Biosciences
catalog: LS-C137481

mouse monoclonal
reactivity: human
application: western blot, ELISA

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ARHGDIA / RHOGDI Antibody LS-C137482
LifeSpan Biosciences
catalog: LS-C137482

mouse monoclonal
reactivity: human
application: western blot, ELISA

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ARHGDIA / RHOGDI Antibody (aa10-19) LS-C139612
LifeSpan Biosciences
catalog: LS-C139612

domestic goat polyclonal
reactivity: human
application: western blot

ARHGDIA antibody (1 ug/ml) staining of Mouse Lung lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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ARHGDIA / RHOGDI Antibody (aa112-140) LS-C156475
LifeSpan Biosciences
catalog: LS-C156475

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, flow cytometry, immunohistochemistry - paraffin section

Western blot of ARHGDIA Antibody in A375(lane 1),HL-60(lane 2),Ramos(lane 3) cell line lysates (35 ug/lane). ARHGDIA (arrow) was detected using the purified antibody.

Formalin-fixed and paraffin-embedded human brain tissue reacted with ARHGDIA Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

ARHGDIA Antibody flow cytometry of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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ARHGDIA / RHOGDI Antibody (clone 2G3) LS-C169101
LifeSpan Biosciences
catalog: LS-C169101

mouse monoclonal (2G3)
reactivity: human
application: western blot, ELISA, immunocytochemistry, flow cytometry

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ARHGDIA / RHOGDI Antibody (clone OTI1A7) LS-C115159
LifeSpan Biosciences
catalog: LS-C115159

mouse monoclonal (OTI1A7)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ARHGDIA (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ARHGDIA.

IHC of paraffin-embedded Adenocarcinoma of colon tissue using anti-ARHGDIA mouse monoclonal antibody. (Dilution 1:50).

Anti-ARHGDIA mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ARHGDIA.
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IHC-plus™ ARHGDIA / RHOGDI Antibody LS-B8714
LifeSpan Biosciences
catalog: LS-B8714

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunoprecipitation, immunohistochemistry - paraffin section

Anti-ARHGDIA / RHOGDI antibody IHC staining of human small intestine, lymphocytes in Peyer's patch. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

WB: NIH 3T3 cell lysate probed with anti-Rho-GDI at 0.5 ug/ml.
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ARHGDIA / RHOGDI Antibody (Biotin) LS-C183097
LifeSpan Biosciences
catalog: LS-C183097

domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA

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ARHGDIA / RHOGDI Antibody (FITC) LS-C183098
LifeSpan Biosciences
catalog: LS-C183098

domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA

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ARHGDIA / RHOGDI Antibody (HRP) LS-C183099
LifeSpan Biosciences
catalog: LS-C183099

domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA

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ARHGDIA / RHOGDI Antibody LS-C189956
LifeSpan Biosciences
catalog: LS-C189956

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA

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ARHGDIA / RHOGDI Antibody (clone 1E12-2G11) LS-C196691
LifeSpan Biosciences
catalog: LS-C196691

mouse monoclonal (1E12-2G11)
reactivity: human
application: ELISA

Detection limit for recombinant GST tagged ARHGDIA is approximately 0.3 ng/ml as a capture antibody.
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ARHGDIA / RHOGDI Antibody (phospho-Ser174) LS-C198642
LifeSpan Biosciences
catalog: LS-C198642

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Immunohistochemistry analysis of paraffin-embedded human cervix carcinoma, using ARHGDIA (Phospho-Ser174) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from HUVEC cells treated with EGF 200ng/ml 30', using ARHGDIA (Phospho-Ser174) Antibody. The lane on the right is blocked with the phospho peptide.
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ARHGDIA / RHOGDI Antibody LS-C211151
LifeSpan Biosciences
catalog: LS-C211151

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry

Western blot of Rho GDP-dissociation inhibitor 1 antibody at 2 ug/ml. Lane 1: EC109 whole cell lysate. Lane 2: 293T whole cell lysate. Secondary: Goat polyclonal to Rabbit IgG at 1:15000 dilution. Predicted band size: 22.4 kDa. Observed band size: 40 kDa. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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ARHGDIA / RHOGDI Antibody (HRP) LS-C211153
LifeSpan Biosciences
catalog: LS-C211153

domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA, immunohistochemistry

Western blot of Rho GDP-dissociation inhibitor 1 antibody at 2 ug/ml. Lane 1: EC109 whole cell lysate. Lane 2: 293T whole cell lysate. Secondary: Goat polyclonal to Rabbit IgG at 1:15000 dilution. Predicted band size: 22.4 kDa. Observed band size: 40 kDa. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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ARHGDIA / RHOGDI Antibody (FITC) LS-C211155
LifeSpan Biosciences
catalog: LS-C211155

domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA, immunohistochemistry

Western blot of Rho GDP-dissociation inhibitor 1 antibody at 2 ug/ml. Lane 1: EC109 whole cell lysate. Lane 2: 293T whole cell lysate. Secondary: Goat polyclonal to Rabbit IgG at 1:15000 dilution. Predicted band size: 22.4 kDa. Observed band size: 40 kDa. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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ARHGDIA / RHOGDI Antibody (Biotin) LS-C211157
LifeSpan Biosciences
catalog: LS-C211157

domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA, immunohistochemistry

Western blot of Rho GDP-dissociation inhibitor 1 antibody at 2 ug/ml. Lane 1: EC109 whole cell lysate. Lane 2: 293T whole cell lysate. Secondary: Goat polyclonal to Rabbit IgG at 1:15000 dilution. Predicted band size: 22.4 kDa. Observed band size: 40 kDa. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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ARHGDIA / RHOGDI Antibody LS-C306332
LifeSpan Biosciences
catalog: LS-C306332

mouse polyclonal
reactivity: human
application: western blot

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ARHGDIA / RHOGDI Antibody LS-C309498
LifeSpan Biosciences
catalog: LS-C309498

domestic rabbit polyclonal
reactivity: human
application: western blot, immunoprecipitation

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ARHGDIA / RHOGDI Antibody LS-C309500
LifeSpan Biosciences
catalog: LS-C309500

domestic rabbit polyclonal
reactivity: human
application: western blot

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ARHGDIA / RHOGDI Antibody LS-C192476
LifeSpan Biosciences
catalog: LS-C192476

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

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ARHGDIA / RHOGDI Antibody LS-C331358
LifeSpan Biosciences
catalog: LS-C331358

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry

Immunofluorescence analysis of HeLa cells.

Western blot analysis of extracts from normal (control) and ARHGDIA knockout (KO) 293T cells, using ARHGDIA antibodyat 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 10s.
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ARHGDIA / RHOGDI Antibody (C-Terminus) LS-C358905
LifeSpan Biosciences
catalog: LS-C358905

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Western blot analysis of RhoGDI alpha (pS174) expression in HeLa (A); Jurkat (B) whole cell lysates.

Immunohistochemical analysis of RhoGDI alpha (pS174) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
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ARHGDIA / RHOGDI Antibody (110-190 aa) LS-C381354
LifeSpan Biosciences
catalog: LS-C381354

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, ELISA, immunohistochemistry

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ARHGDIA / RHOGDI Antibody (110-190 aa) LS-C385855
LifeSpan Biosciences
catalog: LS-C385855

domestic rabbit polyclonal
reactivity: human
application: ELISA, immunohistochemistry

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