IHC-plus™ p56lck / LCK Antibody (clone Lck-01) LS-B1670
LifeSpan Biosciences
catalog: LS-B1670

mouse monoclonal (Lck-01)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section

Anti-LCK antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 15 ug/ml.

Jurkat cell extract was resolved by electrophoresis, transferred to nitrocellulose and probed with monoclonal anti-Lck (clone LCK-01) antibody. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system.
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p56lck / LCK Antibody (aa1-225) LS-C8443
LifeSpan Biosciences
catalog: LS-C8443

mouse monoclonal
reactivity: human, mouse
application: western blot, ELISA, immunoprecipitation

quantity:
price:
to the supplier

p56lck / LCK Antibody (C-Terminus) LS-C8445
LifeSpan Biosciences
catalog: LS-C8445

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunoprecipitation, flow cytometry

quantity:
price:
to the supplier

p56lck / LCK Antibody LS-C8447
LifeSpan Biosciences
catalog: LS-C8447

domestic rabbit polyclonal
reactivity: human
application: western blot, immunoprecipitation

quantity:
price:
to the supplier

p56lck / LCK Antibody (aa1-58) LS-C8450
LifeSpan Biosciences
catalog: LS-C8450

domestic rabbit polyclonal
reactivity: human
application: western blot, immunoprecipitation

quantity:
price:
to the supplier

p56lck / LCK Antibody (aa1-255) LS-C8454
LifeSpan Biosciences
catalog: LS-C8454

mouse monoclonal
reactivity: human
application: western blot, ELISA, immunoprecipitation

quantity:
price:
to the supplier

p56lck / LCK Antibody (phospho-Tyr505) LS-C8455
LifeSpan Biosciences
catalog: LS-C8455

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry, immunoprecipitation, immunohistochemistry - paraffin section

quantity:
price:
to the supplier

p56lck / LCK Antibody (phospho-Tyr505) LS-C8456
LifeSpan Biosciences
catalog: LS-C8456

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot

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to the supplier

p56lck / LCK Antibody (aa1-66) LS-C37157
LifeSpan Biosciences
catalog: LS-C37157

mouse monoclonal
reactivity: human
application: ELISA, immunocytochemistry

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price:
to the supplier

p56lck / LCK Antibody (aa22-36, clone LCK-01) LS-C45900
LifeSpan Biosciences
catalog: LS-C45900

mouse monoclonal (Lck-01)
reactivity: human
application: western blot, immunocytochemistry, immunoprecipitation, flow cytometry

quantity:
price:
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p56lck / LCK Antibody (clone 8E5F9, 4B2C7) LS-C47176
LifeSpan Biosciences
catalog: LS-C47176

mouse monoclonal (8E5F9, 4B2C7)
reactivity: human
application: western blot, ELISA

quantity:
price:
to the supplier

p56lck / LCK Antibody (C-Terminus) LS-C48478
LifeSpan Biosciences
catalog: LS-C48478

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunoprecipitation

quantity:
price:
to the supplier

p56lck / LCK Antibody (phospho-Tyr505) LS-C48479
LifeSpan Biosciences
catalog: LS-C48479

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry, immunoprecipitation

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price:
to the supplier

p56lck / LCK Antibody LS-C71559
LifeSpan Biosciences
catalog: LS-C71559

mouse monoclonal
reactivity: human
application: western blot

quantity:
price:
to the supplier

p56lck / LCK Antibody (aa74-123) LS-C81869
LifeSpan Biosciences
catalog: LS-C81869

domestic rabbit polyclonal
reactivity: human
application: western blot

quantity:
price:
to the supplier

p56lck / LCK Antibody (clone 3A5) LS-C87817
LifeSpan Biosciences
catalog: LS-C87817

mouse monoclonal (3A5)
reactivity: human, mouse
application: western blot, immunoprecipitation

quantity:
price:
to the supplier

p56lck / LCK Antibody (clone 3A5, Azide-free) LS-C87818
LifeSpan Biosciences
catalog: LS-C87818

mouse monoclonal (3A5)
reactivity: human, mouse
application: western blot, immunoprecipitation

quantity:
price:
to the supplier

p56lck / LCK Antibody (aa23-52) LS-C99382
LifeSpan Biosciences
catalog: LS-C99382

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section

Western blot of lysates from mouse thymus and rat thymus tissue lysate(from left to right), using LSK Antibody (I37). Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Confocal immunofluorescence of LCK Antibody with A2058 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
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p56lck / LCK Antibody (phospho-Tyr393) LS-C117405
LifeSpan Biosciences
catalog: LS-C117405

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunocytochemistry

Immunofluorescence analysis of HeLa cells, using Lck (Phospho-Tyr393) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from Jurkat cells, using Lck (Phospho-Tyr393) Antibody. The lane on the right is blocked with the phospho peptide.
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p56lck / LCK Antibody (aa460-509) LS-C117665
LifeSpan Biosciences
catalog: LS-C117665

domestic rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry

Immunofluorescence analysis of HeLa cells, using Lck Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from Jurkat cells, using Lck Antibody. The lane on the right is blocked with the synthesized peptide.
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p56lck / LCK Antibody (aa361-410) LS-C118171
LifeSpan Biosciences
catalog: LS-C118171

domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot analysis of lysates from 293 cells, treated with serum 15% 10', using Lck Antibody. The lane on the right is blocked with the synthesized peptide.
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p56lck / LCK Antibody (aa26-75) LS-C118333
LifeSpan Biosciences
catalog: LS-C118333

domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot analysis of lysates from Jurkat cells, using Lck Antibody. The lane on the right is blocked with the synthesized peptide.
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p56lck / LCK Antibody (aa22-36, clone LCK-01) LS-C130412
LifeSpan Biosciences
catalog: LS-C130412

mouse monoclonal (Lck-01)
reactivity: human
application: western blot, immunoprecipitation

quantity:
price:
to the supplier

p56lck / LCK Antibody (aa1-539) LS-C130413
LifeSpan Biosciences
catalog: LS-C130413

domestic rabbit polyclonal
reactivity: human
application: western blot

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price:
to the supplier

p56lck / LCK Antibody (aa1-66) LS-C143988
LifeSpan Biosciences
catalog: LS-C143988

domestic goat polyclonal
reactivity: human
application: western blot

quantity:
price:
to the supplier

p56lck / LCK Antibody (aa39-52) LS-C154971
LifeSpan Biosciences
catalog: LS-C154971

domestic goat polyclonal
reactivity: human
application: western blot

LCK antibody (0.1 ug/ml) staining of Human Thymus lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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p56lck / LCK Antibody (aa20-47) LS-C164438
LifeSpan Biosciences
catalog: LS-C164438

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot

Western blot of lysate from mouse thymus tissue lysate, using Mouse Lck Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.
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p56lck / LCK Antibody (aa480-509) LS-C165716
LifeSpan Biosciences
catalog: LS-C165716

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, flow cytometry, immunohistochemistry - paraffin section

LCK Antibody western blot of Ramos cell line lysates (35 ug/lane). The LCK antibody detected the LCK protein (arrow).

Formalin-fixed and paraffin-embedded human lymphoma reacted with LCK Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

LCK Antibody flow cytometry of HeLa cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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p56lck / LCK Antibody (Leu387) LS-C177644
LifeSpan Biosciences
catalog: LS-C177644

domestic rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry

Western blot of Lck (L387) pAb in extracts from RAW264.7 cells.
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p56lck / LCK Antibody (Gly534) LS-C177792
LifeSpan Biosciences
catalog: LS-C177792

domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot of Lck (G534) pAb in extracts from Jurkat cells.
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IHC-plus™ p56lck / LCK Antibody LS-B8908
LifeSpan Biosciences
catalog: LS-B8908

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Sample (30 ug of whole cell lysate). A: Molt-4. 7.5% SDS PAGE. LCK antibody diluted at 1:1000.

Anti-p56lck / LCK antibody IHC staining of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
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p56lck / LCK Antibody (clone 3F7-F5) LS-C197606
LifeSpan Biosciences
catalog: LS-C197606

mouse monoclonal (3F7-F5)
reactivity: human
application: western blot, ELISA, immunoprecipitation

Western Blot analysis of LCK expression in transfected 293T cell line by LCK monoclonal antibody (M01), clone 3F7-F5.Lane 1: LCK transfected lysate(59.4 KDa).Lane 2: Non-transfected lysate.

Immunoprecipitation of LCK transfected lysate using anti-LCK monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with LCK rabbit polyclonal antibody.

Detection limit for recombinant GST tagged LCK is approximately 0.1 ng/ml as a capture antibody.
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p56lck / LCK Antibody (phospho-Ser59) LS-C199193
LifeSpan Biosciences
catalog: LS-C199193

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot

Western blot analysis of lysates from Jurkat cells treated with IFN 2500U/ML 30', using LCK (Phospho-Ser59) Antibody. The lane on the right is blocked with the phospho peptide.
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p56lck / LCK Antibody (phospho-Tyr192) LS-C199194
LifeSpan Biosciences
catalog: LS-C199194

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot

Western blot analysis of lysates from Jurkat cells, using Lck (Phospho-Tyr192) Antibody. The lane on the right is blocked with the phospho peptide.
quantity:
price:
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p56lck / LCK Antibody (phospho-Tyr505) LS-C199195
LifeSpan Biosciences
catalog: LS-C199195

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Lck (Phospho-Tyr505) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from Jurkat cells, using Lck (Phospho-Tyr505) Antibody. The lane on the right is blocked with the phospho peptide.
quantity:
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p56lck / LCK Antibody (clone LCK-01, Concentrated) LS-C202153
LifeSpan Biosciences
catalog: LS-C202153

mouse monoclonal (Lck-01)
reactivity: human
application: western blot, immunoprecipitation

quantity:
price:
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p56lck / LCK Antibody (clone 845CT3.5.2) LS-C203937
LifeSpan Biosciences
catalog: LS-C203937

mouse monoclonal (845CT3.5.2)
reactivity: human
application: western blot

LCK Antibody western blot of HL-60,Jurkat,NCI-H292 cell line lysates (35 ug/lane). The LCK antibody detected the LCK protein (arrow).
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p56lck / LCK Antibody (aa21-70) LS-C205809
LifeSpan Biosciences
catalog: LS-C205809

domestic rabbit polyclonal
reactivity: human
application: western blot

quantity:
price:
to the supplier

p56lck / LCK Antibody (aa20-47, AP) LS-C261766
LifeSpan Biosciences
catalog: LS-C261766

domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA

quantity:
price:
to the supplier

p56lck / LCK Antibody (aa20-47, APC) LS-C261767
LifeSpan Biosciences
catalog: LS-C261767

domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA

quantity:
price:
to the supplier

p56lck / LCK Antibody (aa20-47, Biotin) LS-C261768
LifeSpan Biosciences
catalog: LS-C261768

domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA

quantity:
price:
to the supplier

p56lck / LCK Antibody (aa20-47, FITC) LS-C261769
LifeSpan Biosciences
catalog: LS-C261769

domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA

quantity:
price:
to the supplier

p56lck / LCK Antibody (aa20-47, Azide-free, HRP) LS-C261770
LifeSpan Biosciences
catalog: LS-C261770

domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA

quantity:
price:
to the supplier

p56lck / LCK Antibody (aa20-47, PE) LS-C261778
LifeSpan Biosciences
catalog: LS-C261778

domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA

quantity:
price:
to the supplier

p56lck / LCK Antibody (aa23-52, APC) LS-C278878
LifeSpan Biosciences
catalog: LS-C278878

domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA, immunohistochemistry

quantity:
price:
to the supplier

p56lck / LCK Antibody (aa23-52, Biotin) LS-C278879
LifeSpan Biosciences
catalog: LS-C278879

domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA, immunohistochemistry

quantity:
price:
to the supplier

p56lck / LCK Antibody (aa23-52, AP) LS-C278880
LifeSpan Biosciences
catalog: LS-C278880

domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA, immunohistochemistry

quantity:
price:
to the supplier

p56lck / LCK Antibody (aa23-52, FITC) LS-C278881
LifeSpan Biosciences
catalog: LS-C278881

domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA, immunohistochemistry

quantity:
price:
to the supplier

p56lck / LCK Antibody (aa23-52, Azide-free, HRP) LS-C278882
LifeSpan Biosciences
catalog: LS-C278882

domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA, immunohistochemistry

quantity:
price:
to the supplier

p56lck / LCK Antibody (aa23-52, PE) LS-C278883
LifeSpan Biosciences
catalog: LS-C278883

domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA, immunohistochemistry

quantity:
price:
to the supplier

p56lck / LCK Antibody (aa59-108) LS-C292136
LifeSpan Biosciences
catalog: LS-C292136

domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot analysis of the lysates from HeLa cells using LCK antibody.
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p56lck / LCK Antibody LS-C192819
LifeSpan Biosciences
catalog: LS-C192819

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

Western blot of extracts of Jurkat cell lines, using LCK antibody.
quantity:
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p56lck / LCK Antibody (aa20-47) LS-C322613
LifeSpan Biosciences
catalog: LS-C322613

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, ELISA

quantity:
price:
to the supplier

p56lck / LCK Antibody (aa23-52) LS-C327753
LifeSpan Biosciences
catalog: LS-C327753

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry

quantity:
price:
to the supplier

p56lck / LCK Antibody LS-C331981
LifeSpan Biosciences
catalog: LS-C331981

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

Western blot analysis of extracts of various cell lines.
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p56lck / LCK Antibody (phospho-Tyr394) LS-C335849
LifeSpan Biosciences
catalog: LS-C335849

domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot analysis of extracts from JK cells.
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to the supplier

p56lck / LCK Antibody (phospho-Tyr505) LS-C335939
LifeSpan Biosciences
catalog: LS-C335939

domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot analysis of extracts of JK cells.
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price:
to the supplier

p56lck / LCK Antibody LS-C342040
LifeSpan Biosciences
catalog: LS-C342040

mouse monoclonal
reactivity: human
application: western blot

quantity:
price:
to the supplier

p56lck / LCK Antibody (phospho-Tyr393) LS-C352447
LifeSpan Biosciences
catalog: LS-C352447

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunocytochemistry

Western blot analysis of LCK (pY393) expression in MCF7 TNFa-treated (A); mouse liver (B); rat liver (C) whole cell lysates.

Immunofluorescent analysis of LCK (pY393) staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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p56lck / LCK Antibody (Internal) LS-C352453
LifeSpan Biosciences
catalog: LS-C352453

domestic rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry

Western blot analysis of LCK expression in HeLa (A); MCF7 (B); mouse brain (C); mouse heart (D); rat brain (E); rat heart (F) whole cell lysates.

Immunofluorescent analysis of LCK staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
quantity:
price:
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p56lck / LCK Antibody (phospho-Tyr505) LS-C368405
LifeSpan Biosciences
catalog: LS-C368405

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Western blot analysis of LCK (pY505) expression in Jurkat (A); HeLa UV-treated (B) whole cell lysates.

Immunohistochemical analysis of LCK (pY505) staining in human lymphoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
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p56lck / LCK Antibody (aa1-539) LS-C369913
LifeSpan Biosciences
catalog: LS-C369913

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA

quantity:
price:
to the supplier

p56lck / LCK Antibody (aa1-539, HRP) LS-C369914
LifeSpan Biosciences
catalog: LS-C369914

domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA

quantity:
price:
to the supplier

p56lck / LCK Antibody (aa1-539, Biotin) LS-C369917
LifeSpan Biosciences
catalog: LS-C369917

domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA

quantity:
price:
to the supplier

p56lck / LCK Antibody (aa1-539, FITC) LS-C369920
LifeSpan Biosciences
catalog: LS-C369920

domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA

quantity:
price:
to the supplier

p56lck / LCK Antibody (130-210 aa) LS-C380912
LifeSpan Biosciences
catalog: LS-C380912

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, ELISA

quantity:
price:
to the supplier

p56lck / LCK Antibody (330-410 aa) LS-C381176
LifeSpan Biosciences
catalog: LS-C381176

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, ELISA, immunocytochemistry

quantity:
price:
to the supplier

p56lck / LCK Antibody (430-510 aa) LS-C381258
LifeSpan Biosciences
catalog: LS-C381258

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, ELISA, immunohistochemistry

quantity:
price:
to the supplier

p56lck / LCK Antibody (430-510 aa) LS-C384347
LifeSpan Biosciences
catalog: LS-C384347

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunocytochemistry

quantity:
price:
to the supplier

p56lck / LCK Antibody (130-210 aa) LS-C384350
LifeSpan Biosciences
catalog: LS-C384350

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry

quantity:
price:
to the supplier

p56lck / LCK Antibody (330-410 aa) LS-C384352
LifeSpan Biosciences
catalog: LS-C384352

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunocytochemistry

Western blot of Lck antibody
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price:
to the supplier