IHC-plus™ KIF11 / EG5 Antibody LS-B250
LifeSpan Biosciences
catalog: LS-B250
domestic rabbit polyclonal
reactivity: human
application: immunohistochemistry, immunohistochemistry - paraffin section

Anti-KIF11 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.
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KIF11 / EG5 Antibody (clone 10C7/Eg5) LS-C40939
LifeSpan Biosciences
catalog: LS-C40939
mouse monoclonal (10C7/Eg5)
reactivity: human
application: western blot
citations: 1

Hela cell extract (Lane 1) or NIH3T3 cell extract (Lane 2) was resolved by electrophoresis, transferred to nitrocellulose and probed with monoclonal anti-Eg5 (Clone 10C7/Eg5) antibody. Proteins were visualized using a goat anti-mouse secondary conjugated.
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KIF11 / EG5 Antibody LS-C65210
LifeSpan Biosciences
catalog: LS-C65210
domestic rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry, immunoprecipitation
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KIF11 / EG5 Antibody (aa892-941) LS-C117973
LifeSpan Biosciences
catalog: LS-C117973
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Immunofluorescence analysis of HeLa cells, using KIF11/Eg5 Antibody. The picture on the right is blocked with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using KIF11/Eg5 Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from Jurkat, HepG2, and COS cells, using KIF11/Eg5 Antibody. The lane on the right is blocked with the synthesized peptide.
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KIF11 / EG5 Antibody (aa103-130) LS-C165511
LifeSpan Biosciences
catalog: LS-C165511
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, flow cytometry, immunohistochemistry - paraffin section

Western blot of KIF11 Antibody in CHO cell line lysates (35 ug/lane). KIF11 (arrow) was detected using the purified antibody.

Formalin-fixed and paraffin-embedded human lung carcinoma reacted with KIF11 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

KIF11 Antibody flow cytometry of HeLa cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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KIF11 / EG5 Antibody (Pro923) LS-C176538
LifeSpan Biosciences
catalog: LS-C176538
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot of Eg5 (P923) pAb in extracts from COLO205 cells.

IHC of Eg5 (P923) pAb in paraffin-embedded human lung carcinoma tissue.
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KIF11 / EG5 Antibody (clone 2A5) LS-C179844
LifeSpan Biosciences
catalog: LS-C179844
mouse monoclonal (2A5)
reactivity: human
application: immunocytochemistry, immunoprecipitation
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KIF11 / EG5 Antibody (clone 3A1) LS-C179845
LifeSpan Biosciences
catalog: LS-C179845
mouse monoclonal (3A1)
reactivity: human
application: western blot, immunoprecipitation
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KIF11 / EG5 Antibody (aa200-500) LS-C189711
LifeSpan Biosciences
catalog: LS-C189711
mouse monoclonal
reactivity: human
application: western blot, immunocytochemistry, immunoprecipitation
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KIF11 / EG5 Antibody (phospho-Thr926) LS-C199165
LifeSpan Biosciences
catalog: LS-C199165
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot

Western blot analysis of lysates from COLO205 cells, using KIF11/Eg5 (Phospho-Thr926) Antibody. The lane on the right is blocked with the phospho peptide.
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KIF11 / EG5 Antibody LS-C209183
LifeSpan Biosciences
catalog: LS-C209183
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry
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KIF11 / EG5 Antibody (aa103-130, AP) LS-C222980
LifeSpan Biosciences
catalog: LS-C222980
domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA, immunohistochemistry, flow cytometry
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KIF11 / EG5 Antibody (aa103-130, APC) LS-C222981
LifeSpan Biosciences
catalog: LS-C222981
domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA, immunohistochemistry, flow cytometry
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KIF11 / EG5 Antibody (aa103-130, Biotin) LS-C222982
LifeSpan Biosciences
catalog: LS-C222982
domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA, immunohistochemistry, flow cytometry
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KIF11 / EG5 Antibody (aa103-130, FITC) LS-C222983
LifeSpan Biosciences
catalog: LS-C222983
domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA, immunohistochemistry, flow cytometry
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KIF11 / EG5 Antibody (aa103-130, Azide-free, HRP) LS-C222984
LifeSpan Biosciences
catalog: LS-C222984
domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA, immunohistochemistry, flow cytometry
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KIF11 / EG5 Antibody (aa103-130, PE) LS-C222985
LifeSpan Biosciences
catalog: LS-C222985
domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA, immunohistochemistry, flow cytometry
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IHC-plus™ KIF11 / EG5 Antibody LS-B10258
LifeSpan Biosciences
catalog: LS-B10258
mouse monoclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot detection of KIF11 in MCF7,293T & Hela cell lysates using KIF11 antibody (1:1000 diluted). Predicted band size:120KDa. Observed band size:130KDa Additional band size:75K.

Immunocytochemistry stain of Hela using KIF11 antibody (1:200).

Anti-KIF11 / EG5 antibody IHC staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.
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KIF11 / EG5 Antibody (aa925-975) LS-C287907
LifeSpan Biosciences
catalog: LS-C287907
domestic rabbit polyclonal
reactivity: human
application: western blot, immunoprecipitation
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KIF11 / EG5 Antibody (aa1006-C-term) LS-C287909
LifeSpan Biosciences
catalog: LS-C287909
domestic rabbit polyclonal
reactivity: human
application: western blot, immunoprecipitation
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KIF11 / EG5 Antibody (aa103-130) LS-C329727
LifeSpan Biosciences
catalog: LS-C329727
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, flow cytometry
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KIF11 / EG5 Antibody (C-Terminus) LS-C356147
LifeSpan Biosciences
catalog: LS-C356147
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot analysis of EG5 (pT926) expression in Jurkat (A); HEK293T (B) whole cell lysates.

Immunohistochemical analysis of EG5 (pT926) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Immunofluorescent analysis of EG5 (pT926) staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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KIF11 / EG5 Antibody (870-950 aa) LS-C380963
LifeSpan Biosciences
catalog: LS-C380963
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, ELISA

Western blot of Phospho-Eg5 (T926) antibody
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KIF11 / EG5 Antibody (870-950 aa) LS-C383270
LifeSpan Biosciences
catalog: LS-C383270
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry

Western blot of Eg5 antibody
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