ACAT1 Antibody (N-Terminus) LS-C2533
LifeSpan Biosciences
catalog: LS-C2533
domestic rabbit polyclonal
reactivity: human
application: western blot, immunoprecipitation
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IHC-plus™ ACAT1 Antibody (aa218-267) LS-B4679
LifeSpan Biosciences
catalog: LS-B4679
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Anti-ACAT1 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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IHC-plus™ ACAT1 Antibody (aa221-270) LS-B6286
LifeSpan Biosciences
catalog: LS-B6286
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Anti-ACAT1 antibody IHC of human liver, hepatocytes. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.

Western blot analysis of lysates from HepG2, Jurkat, 293, and A549 cells, using ACAT1 Antibody. The lane on the right is blocked with the synthesized peptide.
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ACAT1 Antibody (aa253-266) LS-C108932
LifeSpan Biosciences
catalog: LS-C108932
domestic goat polyclonal
reactivity: human
application: western blot, immunocytochemistry

ACAT1 (aa253-266) antibody (0.1ug/ml) staining of Mouse (A) and Rat (B) Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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ACAT1 Antibody (aa257-269) LS-C112970
LifeSpan Biosciences
catalog: LS-C112970
domestic goat polyclonal
reactivity: human
application: western blot, immunocytochemistry

ACAT1 antibody (0.01 ug/ml) staining of Human Liver lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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ACAT1 Antibody (aa143-192) LS-C116567
LifeSpan Biosciences
catalog: LS-C116567
domestic rabbit polyclonal
reactivity: human
application: western blot
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IHC-plus™ ACAT1 Antibody (N-Terminus, clone AT1.H11) LS-B6358
LifeSpan Biosciences
catalog: LS-B6358
mouse monoclonal (AT1.H11)
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section

Anti-ACAT1 antibody IHC of human adrenal cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
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IHC-plus™ ACAT1 Antibody LS-B6369
LifeSpan Biosciences
catalog: LS-B6369
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section

Anti-ACAT1 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2 ug/ml.
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IHC-plus™ ACAT1 Antibody LS-B6372
LifeSpan Biosciences
catalog: LS-B6372
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Anti-ACAT1 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.
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IHC-plus™ ACAT1 Antibody (aa296-329) LS-B6919
LifeSpan Biosciences
catalog: LS-B6919
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Anti-ACAT1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

Western blot of anti-ACAT1 antibody in SK-BR-3 cell line lysates (35 ug/lane). ACAT1 (arrow) was detected using the purified antibody.
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ACAT1 Antibody LS-C153177
LifeSpan Biosciences
catalog: LS-C153177
domestic rabbit polyclonal
reactivity: human
application: western blot
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ACAT1 Antibody LS-C183422
LifeSpan Biosciences
catalog: LS-C183422
domestic rabbit polyclonal
reactivity: human, rat
application: western blot
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IHC-plus™ ACAT1 Antibody (clone AT2C5) LS-B8975
LifeSpan Biosciences
catalog: LS-B8975
mouse monoclonal (AT2C5)
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section

Western Blot: The cell lysates (40 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACAT1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Anti-ACAT1 antibody IHC staining of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
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ACAT1 Antibody (Lys266) LS-C192106
LifeSpan Biosciences
catalog: LS-C192106
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry
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ACAT1 Antibody (clone 2B7) LS-C196597
LifeSpan Biosciences
catalog: LS-C196597
mouse monoclonal (2B7)
reactivity: human
application: ELISA
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ACAT1 Antibody (aa217-266) LS-C200074
LifeSpan Biosciences
catalog: LS-C200074
domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot analysis of the lysates from HepG2 cells using ACAT1 antibody.
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ACAT1 Antibody LS-C333990
LifeSpan Biosciences
catalog: LS-C333990
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry

Western blot analysis of extracts of various cell lines.

Immunohistochemistry of paraffin-embedded human liver injury tissue.

Immunofluorescence analysis of A549 cells.
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ACAT1 Antibody (aa393-426) LS-C344358
LifeSpan Biosciences
catalog: LS-C344358
domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot of lysates from 293, HepG2, SK-BR-3 cell line (from left to right) with ACAT1 Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35 ug per lane.
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ACAT1 Antibody (Internal) LS-C351787
LifeSpan Biosciences
catalog: LS-C351787
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunoprecipitation, immunohistochemistry - paraffin section

Western blot analysis of ACAT1 expression in HeLa (A); HEK293T (B); SP2/0 (C); H9C2 (D); THP1 (E) whole cell lysates.

Immunohistochemical analysis of ACAT1 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
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ACAT1 Antibody (clone 9H10AB4) LS-C355087
LifeSpan Biosciences
catalog: LS-C355087
mouse monoclonal (9H10AB4)
reactivity: human
application: ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation
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ACAT1 Antibody LS-C356810
LifeSpan Biosciences
catalog: LS-C356810
domestic rabbit polyclonal
reactivity: human
application: western blot

All lanes : Anti-ACAT1 Antibody at 1:1000 dilution Lane 1: HeLa whole cell lysates Lane 2: HepG2 whole cell lysates Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution Predicted band size : 45 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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ACAT1 Antibody (aa31-424) LS-C373192
LifeSpan Biosciences
catalog: LS-C373192
domestic rabbit polyclonal
reactivity: human, mouse
application: western blot

Western blot of ACAT1 antibody.

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ACAT1 Antibody (aa1-145) LS-C375124
LifeSpan Biosciences
catalog: LS-C375124
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section
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ACAT1 Antibody (190-270 aa, Internal) LS-C381872
LifeSpan Biosciences
catalog: LS-C381872
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry

Western blot of ACAT-1 antibody
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