ARAF / ARAF1 / A-RAF Antibody (aa256-286) LS-C97185
LifeSpan Biosciences
catalog: LS-C97185
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Western blot of hARAF1-H270 in MCF7 cell line lysates (35 ug/lane). ARAF1 (arrow) was detected using the purified antibody.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
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ARAF / ARAF1 / A-RAF Antibody (aa254-303) LS-C81879
LifeSpan Biosciences
catalog: LS-C81879
domestic rabbit polyclonal
reactivity: human
application: western blot
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ARAF / ARAF1 / A-RAF Antibody (aa151-251) LS-C131791
LifeSpan Biosciences
catalog: LS-C131791
mouse monoclonal
reactivity: human
application: western blot, ELISA
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IHC-plus™ ARAF / ARAF1 / A-RAF Antibody (aa276-325) LS-B8371
LifeSpan Biosciences
catalog: LS-B8371
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Anti-ARAF / A RAF antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 10 ug/ml.

Western blot analysis of lysates from HeLa cells, treated with PMA 125ng/ml 30', using A-RAF Antibody. The lane on the right is blocked with the synthesized peptide.
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ARAF / ARAF1 / A-RAF Antibody (aa249-277) LS-C164297
LifeSpan Biosciences
catalog: LS-C164297
domestic rabbit polyclonal
reactivity: human, mouse
application: western blot

Mouse Araf Antibody western blot of NCI-H460 cell line lysates (35 ug/lane). The Araf antibody detected the Araf protein (arrow).
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ARAF / ARAF1 / A-RAF Antibody (Asp297) LS-C176384
LifeSpan Biosciences
catalog: LS-C176384
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Western blot of A-RAF (D297) pAb in extracts from HeLa cells treated with PMA 125ng/ml 30'.

IHC of A-RAF (D297) pAb in paraffin-embedded human breast carcinoma tissue.
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ARAF / ARAF1 / A-RAF Antibody (aa371-554) LS-C185401
LifeSpan Biosciences
catalog: LS-C185401
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Sample (30 ug of whole cell lysate). A: NIH-3T3. 7.5% SDS PAGE. ARAF / A RAF antibody diluted at 1:1000.

Immunofluorescence of paraformaldehyde-fixed A431 using ARAF antibody at 1:200 dilution.

IHC of paraffin-embedded Cal27 xenograft using ARAF antibody at 1:500 dilution.
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ARAF / ARAF1 / A-RAF Antibody (clone 6H6) LS-C196681
LifeSpan Biosciences
catalog: LS-C196681
mouse monoclonal (6H6)
reactivity: human
application: western blot, ELISA

ARAF monoclonal antibody (M01), clone 6H6 Western Blot analysis of ARAF expression in HeLa.

Detection limit for recombinant GST tagged ARAF is approximately 0.1 ng/ml as a capture antibody.
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ARAF / ARAF1 / A-RAF Antibody (phospho-Tyr302) LS-C198640
LifeSpan Biosciences
catalog: LS-C198640
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Immunofluorescence analysis of HepG2 cells, using A-RAF (Phospho-Tyr302) Antibody. The picture on the right is blocked with the phospho peptide.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using A-RAF (Phospho-Tyr302) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from HeLa cells treated with PMA 125ng/ml 30', using A-RAF (Phospho-Tyr302) Antibody. The lane on the right is blocked with the phospho peptide.
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ARAF / ARAF1 / A-RAF Antibody (aa226-275) LS-C200140
LifeSpan Biosciences
catalog: LS-C200140
domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot analysis of the lysates from HeLa cells using A-RAF antibody.
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ARAF / ARAF1 / A-RAF Antibody (aa249-277, AP) LS-C251598
LifeSpan Biosciences
catalog: LS-C251598
domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA
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ARAF / ARAF1 / A-RAF Antibody (aa249-277, APC) LS-C251599
LifeSpan Biosciences
catalog: LS-C251599
domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA
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ARAF / ARAF1 / A-RAF Antibody (aa249-277, Biotin) LS-C251600
LifeSpan Biosciences
catalog: LS-C251600
domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA
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ARAF / ARAF1 / A-RAF Antibody (aa249-277, FITC) LS-C251601
LifeSpan Biosciences
catalog: LS-C251601
domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA
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ARAF / ARAF1 / A-RAF Antibody (aa249-277, Azide-free, HRP) LS-C251602
LifeSpan Biosciences
catalog: LS-C251602
domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA
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ARAF / ARAF1 / A-RAF Antibody (aa249-277, PE) LS-C251603
LifeSpan Biosciences
catalog: LS-C251603
domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA
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ARAF / ARAF1 / A-RAF Antibody (aa1-50) LS-C289804
LifeSpan Biosciences
catalog: LS-C289804
domestic rabbit polyclonal
reactivity: human
application: immunoprecipitation
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ARAF / ARAF1 / A-RAF Antibody (aa150-200) LS-C289805
LifeSpan Biosciences
catalog: LS-C289805
domestic rabbit polyclonal
reactivity: human
application: western blot, immunoprecipitation
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ARAF / ARAF1 / A-RAF Antibody (phospho-Ser299) LS-C291182
LifeSpan Biosciences
catalog: LS-C291182
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: immunohistochemistry
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ARAF / ARAF1 / A-RAF Antibody (phospho-Tyr302) LS-C313555
LifeSpan Biosciences
catalog: LS-C313555
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry
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ARAF / ARAF1 / A-RAF Antibody (aa249-277) LS-C329231
LifeSpan Biosciences
catalog: LS-C329231
domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, ELISA
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ARAF / ARAF1 / A-RAF Antibody (aa145-365, clone OTI2G4) LS-C338824
LifeSpan Biosciences
catalog: LS-C338824
mouse monoclonal (OTI2G4)
reactivity: human
application: western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ARAF (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ARAF.
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ARAF / ARAF1 / A-RAF Antibody (aa145-365, clone OTI2G9) LS-C338826
LifeSpan Biosciences
catalog: LS-C338826
mouse monoclonal (OTI2G9)
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ARAF (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ARAF.

IHC of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-ARAF mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
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ARAF / ARAF1 / A-RAF Antibody (aa145-365, clone OTI2F5) LS-C338828
LifeSpan Biosciences
catalog: LS-C338828
mouse monoclonal (OTI2F5)
reactivity: human
application: western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ARAF (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ARAF.
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ARAF / ARAF1 / A-RAF Antibody (aa145-365, clone OTI2F8) LS-C340635
LifeSpan Biosciences
catalog: LS-C340635
mouse monoclonal (OTI2F8)
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ARAF (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ARAF.

IHC of paraffin-embedded Carcinoma of Human prostate tissue using anti-ARAF mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
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ARAF / ARAF1 / A-RAF Antibody (aa356-539) LS-C348823
LifeSpan Biosciences
catalog: LS-C348823
domestic rabbit polyclonal
reactivity: human
application: ELISA, immunohistochemistry
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ARAF / ARAF1 / A-RAF Antibody (phospho-Tyr302) LS-C351844
LifeSpan Biosciences
catalog: LS-C351844
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot

Western blot analysis of A-RAF (pY302) expression in HeLa PMA-treated (A); Raji (B); HT29 (C); NIH3T3 (D); SP2/0 PMA-treated (E); PC12 PMA-treated (F) whole cell lysates.
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ARAF / ARAF1 / A-RAF Antibody (Internal) LS-C358904
LifeSpan Biosciences
catalog: LS-C358904
domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot analysis of A-RAF expression in HeLa (A); HEK293T (B) whole cell lysates.
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ARAF / ARAF1 / A-RAF Antibody (250-330 aa) LS-C381494
LifeSpan Biosciences
catalog: LS-C381494
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, ELISA, immunohistochemistry, immunocytochemistry
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ARAF / ARAF1 / A-RAF Antibody (240-320 aa) LS-C381622
LifeSpan Biosciences
catalog: LS-C381622
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: ELISA, immunohistochemistry
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ARAF / ARAF1 / A-RAF Antibody (250-330 aa) LS-C382096
LifeSpan Biosciences
catalog: LS-C382096
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry

Western blot of A-Raf antibody
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ARAF / ARAF1 / A-RAF Antibody (210-290 aa, Internal) LS-C382097
LifeSpan Biosciences
catalog: LS-C382097
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA

Western blot of A-Raf antibody
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