IHC-plus™ IRF4 Antibody (clone 2F2) LS-B4362
LifeSpan Biosciences
catalog: LS-B4362

mouse monoclonal (2f2)
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section

Western blot of IRF4 expression in transfected 293T cell line by IRF4 monoclonal antibody clone 2F2.

Detection limit for recombinant GST tagged IRF4 is approximately 0.03 ng/ml as a capture antibody.

Anti-IRF-4 antibody IHC of human neutrophils. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
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IRF4 Antibody (aa116-165) LS-C31075
LifeSpan Biosciences
catalog: LS-C31075

domestic rabbit polyclonal
reactivity: human
application: western blot

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IRF4 Antibody (N-Terminus) LS-C80182
LifeSpan Biosciences
catalog: LS-C80182

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

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IRF4 Antibody (C-Terminus) LS-C110245
LifeSpan Biosciences
catalog: LS-C110245

domestic rabbit polyclonal
reactivity: human
application: western blot

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IRF4 Antibody (aa281-330) LS-C119054
LifeSpan Biosciences
catalog: LS-C119054

domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot analysis of lysates from HeLa and Jurkat cells, using IRF4 Antibody. The lane on the right is blocked with the synthesized peptide.
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IRF4 Antibody LS-C130126
LifeSpan Biosciences
catalog: LS-C130126

domestic rabbit monoclonal
reactivity: human
application: western blot, immunoprecipitation

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IHC-plus™ IRF4 Antibody LS-B7104
LifeSpan Biosciences
catalog: LS-B7104

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Anti-IRF-4 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Immunocytochemistry of IRF4 in Jurkat cells with IRF4 antibody at 10 ug/ml.

Immunofluorescence of IRF4 in Jurkat cells with IRF4 antibody at 20 ug/ml.
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IRF4 Antibody (aa433-445) LS-C154961
LifeSpan Biosciences
catalog: LS-C154961

domestic goat polyclonal
reactivity: human
application: western blot

IRF4 antibody (0.5µg/ml) staining of Daudi (A) and Jurkat (B) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
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IRF4 Antibody (aa164-191) LS-C165290
LifeSpan Biosciences
catalog: LS-C165290

domestic rabbit polyclonal
reactivity: human
application: western blot, flow cytometry

Western blot of IRF4 (arrow) using rabbit polyclonal IRF4 Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the IRF4 gene.

IRF4 Antibody flow cytometry of 293 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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IRF4 Antibody (Tyr294) LS-C177125
LifeSpan Biosciences
catalog: LS-C177125

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry

Western blot of IRF-4 (Y294) pAb in extracts from A549 cells.
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IRF4 Antibody (C-Terminus, clone 11J26) LS-C182739
LifeSpan Biosciences
catalog: LS-C182739

rat monoclonal (11J26)
reactivity: human, mouse
application: western blot, flow cytometry

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IRF4 Antibody (aa130-45) LS-C182880
LifeSpan Biosciences
catalog: LS-C182880

domestic sheep polyclonal
reactivity: human
application: western blot, immunohistochemistry

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IRF4 Antibody (aa245-294) LS-C292108
LifeSpan Biosciences
catalog: LS-C292108

domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot analysis of the lysates from HT-29 cells using IRF4 antibody.
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IRF4 Antibody LS-C192325
LifeSpan Biosciences
catalog: LS-C192325

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

Western blot of IRF4 pAb in extracts from HuT-78 cells and mouse lung tissue.
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IRF4 Antibody LS-C331223
LifeSpan Biosciences
catalog: LS-C331223

domestic rabbit polyclonal
reactivity: human
application: immunocytochemistry

Western blot analysis of extracts of various cell lines, using IRF4 antibody. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.

Immunofluorescence analysis of U2OS cells using IRF4 antibody. Blue: DAPI for nuclear staining.
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IRF4 Antibody (aa272-451) LS-C344072
LifeSpan Biosciences
catalog: LS-C344072

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

MUM1 antibody Western blot. All lanes: Anti MUM1 at 0.5 ug/ml. Lane 1: HELA Whole Cell Lysate at 40 ug. Lane 2: JURKAT Whole Cell Lysate at 40 ug. Predicted band size: 51 kD. Observed band size: 40 kD.

MUM1 antibody IHC-paraffin: Human Tonsil Tissue.
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IRF4 Antibody (Internal) LS-C354012
LifeSpan Biosciences
catalog: LS-C354012

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot analysis of IRF4 expression in A549 (A); Ramos (B) whole cell lysates.

Immunohistochemical analysis of IRF4 staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Immunofluorescent analysis of IRF4 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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IRF4 Antibody (aa180-451) LS-C375974
LifeSpan Biosciences
catalog: LS-C375974

domestic rabbit polyclonal
reactivity: human
application: ELISA, immunohistochemistry, immunohistochemistry - paraffin section

Immunohistochemistry of paraffin-embedded human tonsil using antibody at 1:100 dilution.
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IRF4 Antibody (250-330 aa, Internal) LS-C384176
LifeSpan Biosciences
catalog: LS-C384176

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry

Western blot of IRF-4 antibody
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