LifeSpan Biosciences IDH2 antibody

IHC-plus™ IDH2 Antibody (clone 5F11) LS-B4358
LifeSpan Biosciences
catalog: LS-B4358

mouse monoclonal (5F11)
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section

Western blot of IDH2 expression in transfected 293T cell line by IDH2 monoclonal antibody clone 5F11.

Detection limit for recombinant GST tagged IDH2 is approximately 0.03 ng/ml as a capture antibody.

Anti-IDH2 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
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IDH2 Antibody LS-C115860
LifeSpan Biosciences
catalog: LS-C115860

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry

Immunofluorescence of IDH2 in mouse heart tissue with IDH2 antibody at 20 ug/ml.

Western blot analysis of IDH2 in human heart tissue lysate with IDH2 antibody at (A) 1 and (B) 2 ug/ml.

Immunohistochemistry of IDH2 in mouse heart tissue with IDH2 antibody at 5 µg/mL.
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IDH2 Antibody (aa144-193) LS-C102300
LifeSpan Biosciences
catalog: LS-C102300

domestic rabbit polyclonal
reactivity: human
application: western blot

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IHC-plus™ IDH2 Antibody (aa418-429) LS-B8349
LifeSpan Biosciences
catalog: LS-B8349

domestic goat polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

IDH2 antibody (0.1 ug/ml) staining of Human Heart lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-IDH / IDH2 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 3.75 ug/ml.
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IDH2 Antibody (aa194-243) LS-C199893
LifeSpan Biosciences
catalog: LS-C199893

domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot analysis of lysates from Caki-1 cells, using IDH2 antibody.
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IDH2 Antibody (aa402-452) LS-C290743
LifeSpan Biosciences
catalog: LS-C290743

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

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IDH2 Antibody LS-C310687
LifeSpan Biosciences
catalog: LS-C310687

domestic rabbit polyclonal
reactivity: human
application: immunoprecipitation

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IDH2 Antibody LS-C346272
LifeSpan Biosciences
catalog: LS-C346272

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, ChIP-Seq

Western blot analysis of extracts of various cell lines.

Immunohistochemistry of paraffin-embedded human colon carcinoma tissue.

Immunoprecipitation analysis of 200ug extracts of MCF7 cells.
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IDH2 Antibody (Internal) LS-C354399
LifeSpan Biosciences
catalog: LS-C354399

domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot analysis of IDH2 expression in HEK293T (A); Raw264.7 (B); PC12 (C) whole cell lysates.
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IDH2 Antibody (aa375-452) LS-C359331
LifeSpan Biosciences
catalog: LS-C359331

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

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IDH2 Antibody (aa40-452) LS-C373619
LifeSpan Biosciences
catalog: LS-C373619

domestic rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry, immunoprecipitation

Knockout Varification: Lane 1: Wild-type Jurkat cell lysate; Lane 2: IDH2 knockout Jurkat cell lysate; Predicted MW: 51,45kDa ; Observed MW: 45kDa; Primary Ab: 1µg/ml Rabbit Anti-Human IDH2 Antibody; Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody;

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IDH2 Antibody (aa193-452) LS-C375937
LifeSpan Biosciences
catalog: LS-C375937

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section

Western blot. All lanes: IDH2 antibody at 5 ug/ml. Lane 1: HeLa whole cell lysate. Lane 2: mouse heart tissue. Secondary antibody: Goat polyclonal to rabbit at 1:10000 dilution. Predicted band size: 50 kDa. Observed band size: 50 kDa.

Immunohistochemistry of paraffin-embedded human liver cancer using antibody at 1:100 dilution.
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IDH2 Antibody (aa193-452) LS-C375940
LifeSpan Biosciences
catalog: LS-C375940

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA