IDH1 / IDH Antibody (C-Terminus) LS-C81930
LifeSpan Biosciences
catalog: LS-C81930
domestic rabbit polyclonal
reactivity: human
application: western blot
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PathPlus™ IDH1 / IDH Antibody (aa290-339) LS-B3573
LifeSpan Biosciences
catalog: LS-B3573
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Anti-IDH1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

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PathPlus™ IDH1 / IDH Antibody LS-B4836
LifeSpan Biosciences
catalog: LS-B4836
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Sample(30 ug whole cell lysate). A: A431. B: Raji. 10% SDS PAGE. IDH1 antibody diluted at 1:1000.

Anti-IDH1 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
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IDH1 / IDH Antibody (aa63-90) LS-C100657
LifeSpan Biosciences
catalog: LS-C100657
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section

Confocal immunofluorescence of IDH1 Antibody with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Western blot of IDH1 Antibody in mouse liver tissue and 293 cell line lysates (35 ug/lane). IDH1 (arrow) was detected using the purified antibody.

Formalin-fixed and paraffin-embedded human brain reacted with IDH1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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IHC-plus™ IDH1 / IDH Antibody LS-B5288
LifeSpan Biosciences
catalog: LS-B5288
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section

Anti-IDH1 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Western blot analysis of IDH1 in HepG2 cell lysate with IDH1 antibody at (A) 1 and (B) 2 ug/ml.

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IDH1 / IDH Antibody (aa153-164) LS-C139693
LifeSpan Biosciences
catalog: LS-C139693
domestic goat polyclonal
reactivity: human
application: western blot

IDH1 antibody (0.3 ug/ml) staining of HepG2 lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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IDH1 / IDH Antibody (aa65-77) LS-C139694
LifeSpan Biosciences
catalog: LS-C139694
domestic goat polyclonal
reactivity: human
application: western blot

IDH1 antibody (0.1 ug/ml) staining of HepG2 lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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IDH1 / IDH Antibody (aa281-295) LS-C139819
LifeSpan Biosciences
catalog: LS-C139819
domestic goat polyclonal
reactivity: human
application: western blot

IDH1 antibody (0.5 ug/ml) staining of S. cerevisiae S288c lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. The blocking buffer contained non-animal derived protein.
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IDH1 / IDH Antibody LS-C147493
LifeSpan Biosciences
catalog: LS-C147493
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry
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IDH1 / IDH Antibody (Biotin) LS-C147494
LifeSpan Biosciences
catalog: LS-C147494
domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA, immunohistochemistry, immunocytochemistry
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IDH1 / IDH Antibody LS-C147495
LifeSpan Biosciences
catalog: LS-C147495
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry
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IDH1 / IDH Antibody (clone OTI24A2) LS-C175286
LifeSpan Biosciences
catalog: LS-C175286
mouse monoclonal (OTI24A2)
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Western blot of extracts (35 ug) from 9 different cell lines by using anti-IDH1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

IHC of paraffin-embedded Human endometrium tissue using anti-IDH1 mouse monoclonal antibody.
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IDH1 / IDH Antibody LS-C285533
LifeSpan Biosciences
catalog: LS-C285533
domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, ELISA
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IDH1 / IDH Antibody (aa364-414) LS-C290805
LifeSpan Biosciences
catalog: LS-C290805
domestic rabbit polyclonal
reactivity: human
application: western blot, immunoprecipitation
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IDH1 / IDH Antibody (aa1-50) LS-C290806
LifeSpan Biosciences
catalog: LS-C290806
domestic rabbit polyclonal
reactivity: human
application: immunoprecipitation
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IDH1 / IDH Antibody (aa78-330) LS-C294950
LifeSpan Biosciences
catalog: LS-C294950
domestic rabbit polyclonal
reactivity: human, rat
application: western blot

Western blot of recombinant IDH1 / IDH. This image was taken for the unconjugated form of this product. Other forms have not been tested.

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IDH1 / IDH Antibody (aa74-333) LS-C294951
LifeSpan Biosciences
catalog: LS-C294951
domestic rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry, immunoprecipitation

Knockout Varification: Lane 1: Wild-type HepG2 cell lysate; Lane 2: IDH1 knockout HepG2 cell lysate; Predicted MW: 47kDa Observed MW: 47kDa Primary Ab: 2µg/ml Rabbit Anti-Human IDH1 Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody

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IDH1 / IDH Antibody (aa99-325) LS-C294952
LifeSpan Biosciences
catalog: LS-C294952
domestic rabbit polyclonal
reactivity: human, mouse
application: western blot

Western Blot; Sample: Recombinant protein.

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IDH1 / IDH Antibody (aa99-325, Biotin) LS-C299963
LifeSpan Biosciences
catalog: LS-C299963
domestic rabbit polyclonal
reactivity: human, mouse
conjugate: biotin
application: western blot

Western Blot; Sample: Recombinant protein.

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IDH1 / IDH Antibody (aa99-325, FITC) LS-C302531
LifeSpan Biosciences
catalog: LS-C302531
domestic rabbit polyclonal
reactivity: human, mouse
conjugate: FITC
application: western blot

Western Blot; Sample: Recombinant protein.

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IDH1 / IDH Antibody LS-C310790
LifeSpan Biosciences
catalog: LS-C310790
domestic rabbit polyclonal
reactivity: human
application: western blot
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IDH1 / IDH Antibody (Biotin) LS-C317171
LifeSpan Biosciences
catalog: LS-C317171
domestic rabbit polyclonal
reactivity: human, mouse
conjugate: biotin
application: western blot, ELISA
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IDH1 / IDH Antibody (FITC) LS-C317172
LifeSpan Biosciences
catalog: LS-C317172
domestic rabbit polyclonal
reactivity: human, mouse
conjugate: FITC
application: western blot, ELISA
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IDH1 / IDH Antibody (HRP) LS-C318828
LifeSpan Biosciences
catalog: LS-C318828
domestic rabbit polyclonal
reactivity: human, mouse
conjugate: HRP
application: western blot, ELISA
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IDH1 / IDH Antibody LS-C193086
LifeSpan Biosciences
catalog: LS-C193086
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

Western blot of extracts of MCF7 cell lines, using IDH1 antibody.
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IDH1 / IDH Antibody LS-C335214
LifeSpan Biosciences
catalog: LS-C335214
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, ChIP-Seq

Western blot analysis of extracts of various cell lines.

Immunohistochemistry of paraffin-embedded mouse heart.

Immunofluorescence analysis of HeLa cells.
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PathPlus™ IDH1 / IDH Antibody (aa116-143) LS-B11800
LifeSpan Biosciences
catalog: LS-B11800
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section

Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE)

Western blot of lysates from HepG2, MCF-7 cell line, human liver and rat liver tissue lysate(from left to right), using IDH1 Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 35ug per lane.

Confocal immunofluorescence of IDH1 Antibody with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
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IDH1 / IDH Antibody (clone OTI3G9) LS-C336969
LifeSpan Biosciences
catalog: LS-C336969
mouse monoclonal (OTI3G9)
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

HEK293T cells were either not transfected (left lane 293T) or transfected with pCMV6-ENTRY IDH1 (wild type, middle lane WT or pCMV6-ENTRY IDH1 mutated (R132H mutation-SKU# RC400096, right lane R132H) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (10 ug per lane) were separated by SDS-PAGE and immunoblotted with IDH1 antibody (1:500) and then goat anti-mouse IgG-HRP (1:2000).

IHC of paraffin-embedded Human endometrium tissue using anti-IDH1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min).
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IDH1 / IDH Antibody (clone OTI1D1) LS-C336971
LifeSpan Biosciences
catalog: LS-C336971
mouse monoclonal (OTI1D1)
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

HEK293T cells were either not transfected (left lane 293") or transfected with pCMV6-ENTRY IDH1(wild type, middle lane WT) or pCMV6-ENTRY IDH1 mutated (R132H mutation, right lane R132H) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (10 ug per lane) were separated by SDS-PAGE and immunoblotted with IDH1 antibody (1:500) and then goat anti-mouse IgG-HRP (1:2000)

IHC of paraffin-embedded Human pancreas tissue using anti-IDH1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min).
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IDH1 / IDH Antibody (aa1-240) LS-C375949
LifeSpan Biosciences
catalog: LS-C375949
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section

Western blot. All lanes: Isocitrate dehydrogenase [NADP] cytoplasmic antibody at 3 ug/ml. Lane 1: mouse liver tissue. Lane 2: HeLa whole cell lysate. Lane 2: NIH3T3 whole cell lysate. secondary Goat polyclonal to rabbit at 1:10000 dilution. Predicted band size: 47 kDa. Observed band size: 47 kDa.

Immunohistochemistry of paraffin-embedded human colon cancer using antibody at 1:100 dilution.
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IDH1 / IDH Antibody (N-Terminal) LS-C387181
LifeSpan Biosciences
catalog: LS-C387181
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA

Western blot of IDH1 antibody
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IDH1 / IDH Antibody LS-C317734
LifeSpan Biosciences
catalog: LS-C317734
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA

Western blot of Inositol 2-dehydrogenase/D-chiro-inositol 3-dehydrogenase antibody at 2 ug/ml with recombinant Inositol 2-dehydrogenase/D-chiro-inositol 3-dehydrogenase protein 0.1 ug Secondary.
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