IHC-plus™ AIRE Antibody (aa102-115) LS-B1741
LifeSpan Biosciences
catalog: LS-B1741
domestic goat polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Anti-AIRE antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

AIRE antibody staining (0.05µg/ml) of HEK293 cell lysates. Untransfected (Lane 5 and 7) and transfected with Human AIRE (lane 6) or Mouse AIRE (lane 7). Data kindly provided by Prof. Pärt Peterson, University of Tartu, Estonia.
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IHC-plus™ AIRE Antibody (aa534-545) LS-B1740
LifeSpan Biosciences
catalog: LS-B1740
domestic goat polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Anti-AIRE antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

AIRE antibody (1µg/ml) staining of nuclear Jurkat cell (A), (2ug/ml) Human Spleen (B) and negative control Human Smooth Muscle (C) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

AIRE antibody flow cytometric analysis of paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.5% Triton. Primary incubation overnight (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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IHC-plus™ AIRE Antibody (N-Terminus) LS-B11873
LifeSpan Biosciences
catalog: LS-B11873
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE). This image was taken for the unconjugated form of this product. Other forms have not been tested.


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AIRE Antibody (aa76-125) LS-C31748
LifeSpan Biosciences
catalog: LS-C31748
domestic rabbit polyclonal
reactivity: human
application: western blot
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AIRE Antibody (aa159-172) LS-C54468
LifeSpan Biosciences
catalog: LS-C54468
domestic goat polyclonal
reactivity: human
application: western blot

AIRE antibody staining (0.05µg/ml) of HEK293 cell lysates. Untransfected (Lane 5 and 7) and transfected with Human AIRE (lane 6) or Mouse AIRE (lane 7). Data kindly provided by Prof. Pärt Peterson, University of Tartu, Estonia.
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AIRE Antibody (aa218-267) LS-C79983
LifeSpan Biosciences
catalog: LS-C79983
domestic rabbit polyclonal
reactivity: human
application: western blot
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AIRE Antibody (aa76-125) LS-C29968
LifeSpan Biosciences
catalog: LS-C29968
domestic rabbit polyclonal
reactivity: human
application: western blot
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AIRE Antibody (aa467-516) LS-C110232
LifeSpan Biosciences
catalog: LS-C110232
domestic rabbit polyclonal
reactivity: human
application: western blot
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AIRE Antibody (aa91-140) LS-C118687
LifeSpan Biosciences
catalog: LS-C118687
domestic rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry

Immunofluorescence analysis of HeLa cells, using AIRE Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from 293 cells, using AIRE Antibody. The lane on the right is blocked with the synthesized peptide.
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AIRE Antibody (aa476-545) LS-C126647
LifeSpan Biosciences
catalog: LS-C126647
domestic goat polyclonal
reactivity: human
application: western blot, ELISA
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AIRE Antibody (aa476-552) LS-C126648
LifeSpan Biosciences
catalog: LS-C126648
domestic goat polyclonal
reactivity: human, mouse
application: western blot
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AIRE Antibody (aa20-70) LS-C148476
LifeSpan Biosciences
catalog: LS-C148476
domestic rabbit polyclonal
reactivity: human
application: western blot
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AIRE Antibody (aa64-92) LS-C156200
LifeSpan Biosciences
catalog: LS-C156200
domestic rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry

AIRE Antibody western blot of mouse kidney tissue lysates (35 ug/lane). The AIRE antibody detected the AIRE protein (arrow).
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AIRE Antibody (Pro126) LS-C176701
LifeSpan Biosciences
catalog: LS-C176701
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunocytochemistry

Western blot of AIRE-1 (P126) pAb in extracts from 293 cells.
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AIRE Antibody (aa126-175) LS-C198602
LifeSpan Biosciences
catalog: LS-C198602
domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot analysis of lysates from HeLa cells, treated with Hu 2nM 24h, using AIRE Antibody. The lane on the right is blocked with the synthesized peptide.
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AIRE Antibody (phospho-Ser156) LS-C198603
LifeSpan Biosciences
catalog: LS-C198603
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot

Western blot analysis of lysates from HeLa cells treated with Hu 2nM 24h, using AIRE (Phospho-Ser156) Antibody. The lane on the right is blocked with the phospho peptide.
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AIRE Antibody (aa64-92, AP) LS-C251079
LifeSpan Biosciences
catalog: LS-C251079
domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA, immunocytochemistry
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AIRE Antibody (aa64-92, APC) LS-C251080
LifeSpan Biosciences
catalog: LS-C251080
domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA, immunocytochemistry
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AIRE Antibody (aa64-92, Biotin) LS-C251081
LifeSpan Biosciences
catalog: LS-C251081
domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA, immunocytochemistry
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AIRE Antibody (aa64-92, FITC) LS-C251082
LifeSpan Biosciences
catalog: LS-C251082
domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA, immunocytochemistry
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AIRE Antibody (aa64-92, Azide-free, HRP) LS-C251083
LifeSpan Biosciences
catalog: LS-C251083
domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA, immunocytochemistry
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AIRE Antibody (aa64-92, PE) LS-C251085
LifeSpan Biosciences
catalog: LS-C251085
domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA, immunocytochemistry
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AIRE Antibody LS-C193029
LifeSpan Biosciences
catalog: LS-C193029
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

Western blot.
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IHC-plus™ AIRE Antibody LS-B11914
LifeSpan Biosciences
catalog: LS-B11914
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)

Western blot analysis of extracts of various cell lines, using AIRE antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.

Immunofluorescence analysis of A549 cells using AIRE antibody. Blue: DAPI for nuclear staining.
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AIRE Antibody (Internal) LS-C353900
LifeSpan Biosciences
catalog: LS-C353900
domestic rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry

Western blot analysis of AIRE expression in Jurkat (A); mouse lung (B); rat lung (C) whole cell lysates.

Immunofluorescent analysis of AIRE staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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AIRE Antibody (100-180 aa) LS-C381060
LifeSpan Biosciences
catalog: LS-C381060
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, ELISA
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AIRE Antibody (60-140 aa, Internal) LS-C381955
LifeSpan Biosciences
catalog: LS-C381955
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunocytochemistry

Western blot of AIRE-1 antibody
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AIRE Antibody (100-180 aa) LS-C381958
LifeSpan Biosciences
catalog: LS-C381958
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA
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