IHC-plus™ HNF4A / HNF4 Antibody (aa2-15) LS-B3005
LifeSpan Biosciences
catalog: LS-B3005
domestic goat polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

HNF4A / HNF4 antibody (0.5µg/ml) staining of Mouse Small Intestine lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-HNF4A / HNF4 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2 ug/ml.
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IHC-plus™ HNF4A / HNF4 Antibody (N-Terminus) LS-B969
LifeSpan Biosciences
catalog: LS-B969
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Anti-HNF4A / HNF4 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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HNF4A / HNF4 Antibody (Ser142) LS-C97257
LifeSpan Biosciences
catalog: LS-C97257
domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot of anti-HNF4A Antibody (S142) in NCI-H460 cell line lysates (35 ug/lane). HNF4A (arrow) was detected using the purified antibody.
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HNF4A / HNF4 Antibody (aa281-312) LS-C97258
LifeSpan Biosciences
catalog: LS-C97258
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section

HNF4A Antibody western blot of 293,HepG2 cell line and rat liver tissue lysates (35 ug/lane). The HNF4A antibody detected the HNF4A protein (arrow).

HNF4A Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human kidney tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

Confocal immunofluorescence of HNF4A Antibody with HeLa cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
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LXR Alpha+Beta Antibody (Internal) LS-C73782
LifeSpan Biosciences
catalog: LS-C73782
domestic rabbit polyclonal
reactivity: human
application: western blot
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HNF4A / HNF4 Antibody (aa252-301) LS-C111045
LifeSpan Biosciences
catalog: LS-C111045
domestic rabbit polyclonal
reactivity: human
application: western blot
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IHC-plus™ HNF4A / HNF4 Antibody (C-Terminus) LS-B4568
LifeSpan Biosciences
catalog: LS-B4568
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Anti-HNF4A / HNF4 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

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HNF4A / HNF4 Antibody (phospho-Ser313) LS-C117375
LifeSpan Biosciences
catalog: LS-C117375
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Immunofluorescence analysis of LOVO cells, using HNF4 alpha (Phospho-Ser313) Antibody. The picture on the right is blocked with the phospho peptide.

Immunohistochemistry analysis of paraffin-embedded human liver carcinoma, using HNF4 alpha (Phospho-Ser313) Antibody. The picture on the right is blocked with the phospho peptide.
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HNF4A / HNF4 Antibody (aa280-329) LS-C118140
LifeSpan Biosciences
catalog: LS-C118140
domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot analysis of the lysates from HepG2 cells using HNF4alpha antibody.
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HNF4A / HNF4 Antibody LS-C129706
LifeSpan Biosciences
catalog: LS-C129706
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA
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HNF4A / HNF4 Antibody LS-C129707
LifeSpan Biosciences
catalog: LS-C129707
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA
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LXR Alpha+Beta Antibody (C-Terminus) LS-C131112
LifeSpan Biosciences
catalog: LS-C131112
domestic rabbit polyclonal
reactivity: human
application: western blot
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HNF4A / HNF4 Antibody LS-C146867
LifeSpan Biosciences
catalog: LS-C146867
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA
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HNF4A / HNF4 Antibody LS-C146868
LifeSpan Biosciences
catalog: LS-C146868
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA
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HNF4A / HNF4 Antibody LS-C146943
LifeSpan Biosciences
catalog: LS-C146943
domestic rabbit polyclonal
reactivity: human, rat
application: western blot
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HNF4A / HNF4 Antibody (aa107-139) LS-C164947
LifeSpan Biosciences
catalog: LS-C164947
domestic rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry

Western blot of HNF4A Antibody in 293 cell line lysates (35 ug/lane). HNF4A (arrow) was detected using the purified antibody.

Confocal immunofluorescence of HNF4A Antibody with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
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HNF4A / HNF4 Antibody (Lys298) LS-C177317
LifeSpan Biosciences
catalog: LS-C177317
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

Western blot of HNF4 (K298) pAb in extracts from HT-29 cells.
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HNF4A / HNF4 Antibody (phospho-Ser313) LS-C192225
LifeSpan Biosciences
catalog: LS-C192225
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry
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HNF4A / HNF4 Antibody (clone 4E2) LS-C197427
LifeSpan Biosciences
catalog: LS-C197427
mouse monoclonal (4E2)
reactivity: human
application: western blot, ELISA, immunocytochemistry

Western Blot analysis of HNF4A expression in transfected 293T cell line by HNF4A monoclonal antibody (M04), clone 4E2.Lane 1: HNF4A transfected lysate(51.6 KDa).Lane 2: Non-transfected lysate.

Immunofluorescence of monoclonal antibody to HNF4A on HeLa cell. [antibody concentration 10 ug/ml]

Detection limit for recombinant GST tagged HNF4A is approximately 1 ng/ml as a capture antibody.
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HNF4A / HNF4 Antibody (Ser142, AP) LS-C277997
LifeSpan Biosciences
catalog: LS-C277997
domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA
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HNF4A / HNF4 Antibody (Ser142, APC) LS-C277998
LifeSpan Biosciences
catalog: LS-C277998
domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA
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HNF4A / HNF4 Antibody (Ser142, Biotin) LS-C277999
LifeSpan Biosciences
catalog: LS-C277999
domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA
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HNF4A / HNF4 Antibody (Ser142, FITC) LS-C278000
LifeSpan Biosciences
catalog: LS-C278000
domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA
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HNF4A / HNF4 Antibody (Ser142, Azide-free, HRP) LS-C278001
LifeSpan Biosciences
catalog: LS-C278001
domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA
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HNF4A / HNF4 Antibody (Ser142, PE) LS-C278002
LifeSpan Biosciences
catalog: LS-C278002
domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA
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HNF4A / HNF4 Antibody (aa258-307) LS-C286286
LifeSpan Biosciences
catalog: LS-C286286
domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot analysis of lysates from HT-29 cells, using HNF4 alpha Antibody. The lane on the right is blocked with the synthesized peptide.
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HNF4A / HNF4 Antibody LS-C310633
LifeSpan Biosciences
catalog: LS-C310633
domestic rabbit polyclonal
reactivity: human
application: western blot, immunoprecipitation
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HNF4A / HNF4 Antibody LS-C310634
LifeSpan Biosciences
catalog: LS-C310634
domestic rabbit polyclonal
reactivity: human
application: western blot
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HNF4A / HNF4 Antibody LS-C193056
LifeSpan Biosciences
catalog: LS-C193056
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

Western blot of extracts of various cell lines, using HNF4A antibody.
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HNF4A / HNF4 Antibody (Ser142) LS-C327541
LifeSpan Biosciences
catalog: LS-C327541
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA
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HNF4A / HNF4 Antibody LS-C331883
LifeSpan Biosciences
catalog: LS-C331883
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry

Western blot analysis of extracts of various cell lines.

Immunofluorescence analysis.
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HNF4A / HNF4 Antibody (phospho-Ser304) LS-C336002
LifeSpan Biosciences
catalog: LS-C336002
domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot analysis of extracts from Hela cells.
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HNF4A / HNF4 Antibody (aa1-315, clone OTI8B8) LS-C340013
LifeSpan Biosciences
catalog: LS-C340013
mouse monoclonal (OTI8B8)
reactivity: human
application: western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HNF4A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HNF4A.
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HNF4A / HNF4 Antibody (aa1-315, clone OTI9H8) LS-C340016
LifeSpan Biosciences
catalog: LS-C340016
mouse monoclonal (OTI9H8)
reactivity: human
application: western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HNF4A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HNF4A.
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HNF4A / HNF4 Antibody (aa1-315, clone OTI2H2) LS-C340017
LifeSpan Biosciences
catalog: LS-C340017
mouse monoclonal (OTI2H2)
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HNF4A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HNF4A.

IHC of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-HNF4A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
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HNF4A / HNF4 Antibody (aa1-315, clone OTI5A9) LS-C340018
LifeSpan Biosciences
catalog: LS-C340018
mouse monoclonal (OTI5A9)
reactivity: human
application: western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HNF4A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HNF4A.
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HNF4A / HNF4 Antibody (aa1-315, clone OTI2C10) LS-C340020
LifeSpan Biosciences
catalog: LS-C340020
mouse monoclonal (OTI2C10)
reactivity: human
application: western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HNF4A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HNF4A.
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HNF4A / HNF4 Antibody (phospho-Ser313) LS-C354006
LifeSpan Biosciences
catalog: LS-C354006
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot analysis of HNF4 alpha (pS313) expression in SW480 (A); HepG2 EGF-treated (B) whole cell lysates.

Immunohistochemical analysis of HNF4 alpha (pS313) staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Immunofluorescent analysis of HNF4 alpha (pS313) staining in SW480 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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HNF4A / HNF4 Antibody (250-330 aa) LS-C381547
LifeSpan Biosciences
catalog: LS-C381547
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, ELISA, immunohistochemistry, immunocytochemistry

Western blot of Phospho-HNF4-alpha (S313) antibody
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HNF4A / HNF4 Antibody (250-330 aa) LS-C383969
LifeSpan Biosciences
catalog: LS-C383969
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry

Western blot of HNF-4alpha antibody
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