HARS Antibody LS-C24926
LifeSpan Biosciences
catalog: LS-C24926
human polyclonal
reactivity: human
application: ELISA, immunoprecipitation
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HARS Antibody (aa49-78) LS-C100191
LifeSpan Biosciences
catalog: LS-C100191
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Western blot of anti-HARS antibody in K562 cell line lysates (35 ug/lane). HARS (arrow) was detected using the purified antibody.

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with HARS antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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HARS Antibody LS-C67926
LifeSpan Biosciences
catalog: LS-C67926
mouse monoclonal
reactivity: human
application: western blot, immunocytochemistry, immunoprecipitation, flow cytometry
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HARS Antibody LS-C130309
LifeSpan Biosciences
catalog: LS-C130309
human polyclonal
reactivity: human
application: ELISA
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HARS Antibody (aa54-103) LS-C135959
LifeSpan Biosciences
catalog: LS-C135959
domestic rabbit polyclonal
reactivity: human
application: western blot
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HARS Antibody (Glu492) LS-C176729
LifeSpan Biosciences
catalog: LS-C176729
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot of HisRS (E492) pAb in extracts from raw264.7 cells.

IHC of HisRS (E492) pAb in paraffin-embedded human brain tissue.
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HARS Antibody (clone HARSA6) LS-C184288
LifeSpan Biosciences
catalog: LS-C184288
mouse monoclonal (HARSA6)
reactivity: human
application: western blot, immunocytochemistry, immunoprecipitation, flow cytometry
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IHC-plus™ HARS Antibody (aa460-509) LS-B9136
LifeSpan Biosciences
catalog: LS-B9136
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Human, Small intestine: Formalin-Fixed Paraffin-Embedded (FFPE)

Immunofluorescence analysis of HepG2 and HeLa cells, using HARS Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from COLO cells, using HARS Antibody. The lane on the right is blocked with the synthesized peptide.
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HARS Antibody (clone 1C8) LS-C197396
LifeSpan Biosciences
catalog: LS-C197396
mouse monoclonal (1C8)
reactivity: human
application: western blot, ELISA, immunocytochemistry

HARS monoclonal antibody (M01), clone 1C8 Western Blot analysis of HARS expression in HeLa.

Immunofluorescence of monoclonal antibody to HARS on HeLa cell. [antibody concentration 10 ug/ml]

Detection limit for recombinant GST tagged HARS is approximately 0.03 ng/ml as a capture antibody.
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HARS Antibody LS-C308183
LifeSpan Biosciences
catalog: LS-C308183
mouse polyclonal
reactivity: human
application: western blot, immunocytochemistry
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HARS Antibody LS-C310572
LifeSpan Biosciences
catalog: LS-C310572
domestic rabbit polyclonal
reactivity: human
application: immunoprecipitation
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HARS Antibody (clone HARSA6) LS-C342343
LifeSpan Biosciences
catalog: LS-C342343
mouse monoclonal (HARSA6)
reactivity: human
application: western blot, immunocytochemistry, immunoprecipitation, flow cytometry

Western blot analysis of immunized recombinant protein, using anti-HARS monoclonal antibody.

Immunoprecipitation: RIPA lysate of HeLa cells was incubated with anti-HARS mAb. Predicted molecular weight: 57 kDa

Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-HARS mAb.
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HARS Antibody (C-Terminus) LS-C353997
LifeSpan Biosciences
catalog: LS-C353997
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot analysis of HARS expression in K562 (A); Raw264.7 (B) whole cell lysates.

Immunohistochemical analysis of HARS staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Immunofluorescent analysis of HARS staining in K562 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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HARS Antibody (aa1-180) LS-C375873
LifeSpan Biosciences
catalog: LS-C375873
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section

Western blot. All lanes: Histidine--tRNA ligase, cytoplasmic antibody at 6 ug/ml. Lane 1: HeLa whole cell lysate. Lane 2: K562 whole cell lysate. Lane 3: 293T whole cell lysate. secondary Goat polyclonal to rabbit at 1:10000 dilution. Predicted band size: 57 kDa. Observed band size: 57 kDa.

Immunohistochemistry of paraffin-embedded human liver cancer using antibody at 1:100 dilution.
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HARS Antibody (430-510 aa, C-terminal) LS-C383922
LifeSpan Biosciences
catalog: LS-C383922
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry

Western blot of HisRS antibody
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