H2AFX / H2AX Antibody (phospho-Ser139, clone 2F3) LS-C41114
LifeSpan Biosciences
catalog: LS-C41114

mouse monoclonal (2F3)
reactivity: human
modification: phosphorylated
application: western blot, immunocytochemistry
citations: 5

Untreated (Lane 1) and staurosporine-treated (Lane 2) Jurkat nuclear extract was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-phospho-H2A. X (Ser 139) antibody. Proteins were visualized using a goat anti-mouse secondary a.

Untreated Hela cells (Panel A), or overnight nocodazole treated Hela cells (Panel B) stained with purified mouse monoclonal antibody against Ser139 phosphorylated H2A.X, followed by Rhodamine Red-X conjugated Donkey anti-mouse IgG and DAPI.
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H2AFX / H2AX Antibody (aa121-135) LS-C7422
LifeSpan Biosciences
catalog: LS-C7422

domestic rabbit polyclonal
reactivity: human
application: western blot

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H2AFX / H2AX Antibody (phospho-Ser139, Biotin) LS-C7423
LifeSpan Biosciences
catalog: LS-C7423

mouse monoclonal
reactivity: human
conjugate: biotin
modification: phosphorylated
application: western blot

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H2AFX / H2AX Antibody (phospho-Ser139) LS-C7424
LifeSpan Biosciences
catalog: LS-C7424

mouse monoclonal
reactivity: human
modification: phosphorylated
application: western blot, immunocytochemistry, immunoprecipitation

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H2AFX / H2AX Antibody LS-C48684
LifeSpan Biosciences
catalog: LS-C48684

domestic rabbit polyclonal
reactivity: human
application: western blot

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H2AFX / H2AX Antibody (N-Terminus) LS-C102762
LifeSpan Biosciences
catalog: LS-C102762

domestic rabbit polyclonal
reactivity: human
application: western blot

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IHC-plus™ H2AFX / H2AX Antibody (C-Terminus) LS-B3282
LifeSpan Biosciences
catalog: LS-B3282

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Anti-H2AX antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
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H2AFX / H2AX Antibody (phospho-Ser139) LS-C117373
LifeSpan Biosciences
catalog: LS-C117373

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot

Western blot analysis of lysates from 293 cells treated with heat shock, using Histone H2A.X (Phospho-Ser139) Antibody. The lane on the right is blocked with the phospho peptide.
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H2AFX / H2AX Antibody (aa94-143) LS-C118792
LifeSpan Biosciences
catalog: LS-C118792

domestic rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry

Immunofluorescence analysis of COS7 cells, using Histone H2AXÂ Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from HT-29 cells, using Histone H2AXÂ Antibody. The lane on the right is blocked with the synthesized peptide.
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H2AFX / H2AX Antibody LS-C123758
LifeSpan Biosciences
catalog: LS-C123758

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry

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H2AFX / H2AX Antibody (phospho-Ser139) LS-C129793
LifeSpan Biosciences
catalog: LS-C129793

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot

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IHC-plus™ H2AFX / H2AX Antibody (C-Terminus) LS-B6918
LifeSpan Biosciences
catalog: LS-B6918

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Anti-H2AX antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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H2AFX / H2AX Antibody (C-Terminus) LS-C153461
LifeSpan Biosciences
catalog: LS-C153461

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry

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H2AFX / H2AX Antibody (aa111-140) LS-C166760
LifeSpan Biosciences
catalog: LS-C166760

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

H2AFX Antibody western blot of K562 cell line lysates (35 ug/lane). The H2AFX antibody detected the H2AFX protein (arrow).

H2AFX Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human tonsil tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
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H2AFX / H2AX Antibody (Ser130) LS-C177318
LifeSpan Biosciences
catalog: LS-C177318

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot of Histone H2A.X (S130) pAb in extracts from HT-29 cells.

IHC of Histone H2A.X (S130) pAb in paraffin-embedded human breast carcinoma tissue.
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H2AFX / H2AX Antibody (phospho-Ser139) LS-C178034
LifeSpan Biosciences
catalog: LS-C178034

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Western blot of p-Histone H2A.X (S139) pAb in extracts from HeLa UV cells.

IHC of p-Histone H2A.X (S139) pAb in paraffin-embedded human brain tissue.
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H2AFX / H2AX Antibody (phospho-Ser139) LS-C178091
LifeSpan Biosciences
catalog: LS-C178091

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Western blot of p-Histone H2A.X (S139) pAb in extracts from 293 neocarzinostatin cells.

IHC of p-Histone H2A.X (S139) pAb in paraffin-embedded human malignant lymphoma tissue.
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H2AFX / H2AX Antibody (Glu145) LS-C178177
LifeSpan Biosciences
catalog: LS-C178177

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Western blot of Histone H2A.X (E145) pAb in extracts from HeLa cells.

IHC of Histone H2A.X (E145) pAb in paraffin-embedded human malignant lymphoma tissue.
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H2AFX / H2AX Antibody (phospho-Ser139, clone 20E3, PE) LS-C183907
LifeSpan Biosciences
catalog: LS-C183907

domestic rabbit monoclonal (20E3)
reactivity: human
conjugate: PE
modification: phosphorylated
application: flow cytometry

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H2AFX / H2AX Antibody (C-Terminus) LS-C190204
LifeSpan Biosciences
catalog: LS-C190204

domestic rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry

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H2AFX / H2AX Antibody (clone 3F4) LS-C197392
LifeSpan Biosciences
catalog: LS-C197392

mouse monoclonal (3F4)
reactivity: human
application: ELISA, immunocytochemistry

Immunofluorescence of monoclonal antibody to H2AFX on HeLa cell . [antibody concentration 10 ug/ml]
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H2AFX / H2AX Antibody (aa115-143, clone 938CT5.1.1) LS-C203985
LifeSpan Biosciences
catalog: LS-C203985

mouse monoclonal (938CT5.1.1)
reactivity: human
application: western blot

Western blot of lysates from 293, CEM, HepG2, Jurkat, HeLa, Raji cell line(from left to right). using H2AFX Antibody. Antibody was diluted at 1:2000 at each lane. A goat anti-mouse IgG H&L (HRP) at 1:3000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
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H2AFX / H2AX Antibody (aa1-30, clone 980CT1.5.5) LS-C204027
LifeSpan Biosciences
catalog: LS-C204027

mouse monoclonal (980CT1.5.5)
reactivity: human
application: western blot

H2AFX Antibody western blot of CEM,HepG2,Raji cell line lysates (35 ug/lane). The H2AFX antibody detected the H2AFX protein (arrow).
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H2AFX / H2AX Antibody (phospho-Ser139) LS-C209342
LifeSpan Biosciences
catalog: LS-C209342

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, ELISA

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H2AFX / H2AX Antibody (HRP) LS-C211527
LifeSpan Biosciences
catalog: LS-C211527

domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA

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H2AFX / H2AX Antibody (FITC) LS-C211528
LifeSpan Biosciences
catalog: LS-C211528

domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA

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H2AFX / H2AX Antibody (Biotin) LS-C211531
LifeSpan Biosciences
catalog: LS-C211531

domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA

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H2AFX / H2AX Antibody (aa111-140, APC) LS-C258212
LifeSpan Biosciences
catalog: LS-C258212

domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA, immunohistochemistry

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H2AFX / H2AX Antibody (aa111-140, AP) LS-C258214
LifeSpan Biosciences
catalog: LS-C258214

domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA, immunohistochemistry

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H2AFX / H2AX Antibody (aa111-140, PE) LS-C258215
LifeSpan Biosciences
catalog: LS-C258215

domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA, immunohistochemistry

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H2AFX / H2AX Antibody (C-Terminus) LS-C285928
LifeSpan Biosciences
catalog: LS-C285928

domestic rabbit polyclonal
reactivity: human
application: immunohistochemistry

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H2AFX / H2AX Antibody (phospho-Ser139) LS-C286334
LifeSpan Biosciences
catalog: LS-C286334

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: immunohistochemistry, immunocytochemistry

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H2AFX / H2AX Antibody (phospho-Ser140) LS-C287018
LifeSpan Biosciences
catalog: LS-C287018

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry, immunocytochemistry

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H2AFX / H2AX Antibody (C-Terminus) LS-C287020
LifeSpan Biosciences
catalog: LS-C287020

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

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H2AFX / H2AX Antibody (C-Terminus) LS-C287021
LifeSpan Biosciences
catalog: LS-C287021

domestic rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry

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H2AFX / H2AX Antibody (C-Terminus) LS-C290487
LifeSpan Biosciences
catalog: LS-C290487

domestic goat polyclonal
reactivity: human
application: western blot, immunohistochemistry

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H2AFX / H2AX Antibody (aa2-143) LS-C294375
LifeSpan Biosciences
catalog: LS-C294375

domestic rabbit polyclonal
reactivity: human
application: western blot

Western Blot; Lane1: Human 293T Cells; Lane2: Human Raji Cells.

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H2AFX / H2AX Antibody LS-C311206
LifeSpan Biosciences
catalog: LS-C311206

domestic rabbit polyclonal
reactivity: human
application: western blot

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H2AFX / H2AX Antibody (aa111-140) LS-C321861
LifeSpan Biosciences
catalog: LS-C321861

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry

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H2AFX / H2AX Antibody (Ser139) LS-C344324
LifeSpan Biosciences
catalog: LS-C344324

domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot of lysates from 293, K562, Raji cell line (from left to right) with Histone H2A. X (Ser139). Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35 ug per lane.
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H2AFX / H2AX Antibody (phospho-Ser139, clone 20E3) LS-C348689
LifeSpan Biosciences
catalog: LS-C348689

domestic rabbit monoclonal (20E3)
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry

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H2AFX / H2AX Antibody LS-C348958
LifeSpan Biosciences
catalog: LS-C348958

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

Western blot analysis of extracts of various cell lines.
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H2AFX / H2AX Antibody (C-Terminus) LS-C352308
LifeSpan Biosciences
catalog: LS-C352308

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot analysis of Histone H2A.X expression in HeLa (A); MCF7 (B); Jurkat (C) whole cell lysates.

Immunohistochemical analysis of Histone H2A.X staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Immunofluorescent analysis of Histone H2A.X staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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H2AFX / H2AX Antibody (C-Terminus) LS-C358767
LifeSpan Biosciences
catalog: LS-C358767

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot analysis of Histone H2A.X expression in HepG2 (A); mouse brain (B) whole cell lysates.

Immunohistochemical analysis of Histone H2A.X staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Immunofluorescent analysis of Histone H2A.X staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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H2AFX / H2AX Antibody (C-Terminus) LS-C368744
LifeSpan Biosciences
catalog: LS-C368744

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section

Western blot analysis of Histone H2A.X (pS139) expression in HEK293T (A); NIH3T3 (B) whole cell lysates.

Immunohistochemical analysis of Histone H2A.X (pS139) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected with HRP and DAB as chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
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H2AFX / H2AX Antibody (aa1-143) LS-C375895
LifeSpan Biosciences
catalog: LS-C375895

domestic rabbit polyclonal
reactivity: human
application: ELISA, immunohistochemistry, immunohistochemistry - paraffin section

Immunohistochemistry of paraffin-embedded human testis using antibody at 1:100 dilution.
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H2AFX / H2AX Antibody (80-160 aa) LS-C380728
LifeSpan Biosciences
catalog: LS-C380728

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, ELISA, immunohistochemistry

Western blot of Phospho-Histone H2A.X (S139) antibody
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H2AFX / H2AX Antibody (70-150 aa, C-terminal) LS-C383938
LifeSpan Biosciences
catalog: LS-C383938

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry

Western blot of Histone H2A.X antibody
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H2AFX / H2AX Antibody (80-160 aa) LS-C383940
LifeSpan Biosciences
catalog: LS-C383940

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry

Western blot of Histone H2A.X antibody
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H2AFX / H2AX Antibody (phospho-Thr120) LS-C387575
LifeSpan Biosciences
catalog: LS-C387575

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot

Western blot of Histone H2A.X (Phospho-Thr120) antibody
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H2AFX / H2AX Antibody (phospho-Ser139) LS-C387576
LifeSpan Biosciences
catalog: LS-C387576

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot

Western blot of Histone H2A.X (Phospho-Ser139) antibody
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H2AFX / H2AX Antibody LS-C387580
LifeSpan Biosciences
catalog: LS-C387580

domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot of Histone H2A.X (Acetyl Lys5) antibody
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