IHC-plus™ GTF2I / TFII I Antibody LS-B3097
LifeSpan Biosciences
catalog: LS-B3097
domestic rabbit polyclonal
reactivity: human
application: ELISA, immunohistochemistry, immunohistochemistry - paraffin section

Anti-TFII-I antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

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GTF2I / TFII I Antibody (aa208-257) LS-C29903
LifeSpan Biosciences
catalog: LS-C29903
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section
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GTF2I / TFII I Antibody (aa208-257) LS-C31747
LifeSpan Biosciences
catalog: LS-C31747
domestic rabbit polyclonal
reactivity: human
application: western blot
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GTF2I / TFII I Antibody (C-Terminus) LS-C102734
LifeSpan Biosciences
catalog: LS-C102734
domestic rabbit polyclonal
reactivity: human
application: western blot
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GTF2I / TFII I Antibody (aa281-310) LS-C159799
LifeSpan Biosciences
catalog: LS-C159799
domestic rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry

Fluorescent image of HeLa cell stained with GTF2I Antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with GTF2I primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). GTF2I immunoreactivity is localized to Nucleus significantly.

GTF2I Antibody western blot of 293 cell line lysates (35 ug/lane). The GTF2I antibody detected the GTF2I protein (arrow).
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GTF2I / TFII I Antibody (clone 354C4a) LS-C184813
LifeSpan Biosciences
catalog: LS-C184813
mouse monoclonal (354C4a)
reactivity: human
application: immunocytochemistry
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GTF2I / TFII I Antibody (phospho-Tyr248) LS-C199646
LifeSpan Biosciences
catalog: LS-C199646
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot

Western blot analysis of lysates from LOVO cells, using TFII-I (Phospho-Tyr248) Antibody. The lane on the right is blocked with the phospho peptide.
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GTF2I / TFII I Antibody (aa281-310, AP) LS-C258162
LifeSpan Biosciences
catalog: LS-C258162
domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA
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GTF2I / TFII I Antibody (aa281-310, APC) LS-C258163
LifeSpan Biosciences
catalog: LS-C258163
domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA
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GTF2I / TFII I Antibody (aa281-310, Biotin) LS-C258164
LifeSpan Biosciences
catalog: LS-C258164
domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA
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GTF2I / TFII I Antibody (aa281-310, FITC) LS-C258165
LifeSpan Biosciences
catalog: LS-C258165
domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA
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GTF2I / TFII I Antibody (aa281-310, Azide-free, HRP) LS-C258166
LifeSpan Biosciences
catalog: LS-C258166
domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA
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GTF2I / TFII I Antibody (aa281-310, PE) LS-C258168
LifeSpan Biosciences
catalog: LS-C258168
domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA
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GTF2I / TFII I Antibody (aa956-985, APC) LS-C283497
LifeSpan Biosciences
catalog: LS-C283497
domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA
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GTF2I / TFII I Antibody (aa956-985, AP) LS-C283499
LifeSpan Biosciences
catalog: LS-C283499
domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA
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GTF2I / TFII I Antibody (aa956-985, Biotin) LS-C283500
LifeSpan Biosciences
catalog: LS-C283500
domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA
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GTF2I / TFII I Antibody (aa956-985, FITC) LS-C283501
LifeSpan Biosciences
catalog: LS-C283501
domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA
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GTF2I / TFII I Antibody (aa956-985, Azide-free, HRP) LS-C283502
LifeSpan Biosciences
catalog: LS-C283502
domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA
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GTF2I / TFII I Antibody (aa956-985, PE) LS-C283504
LifeSpan Biosciences
catalog: LS-C283504
domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA
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GTF2I / TFII I Antibody (aa950-C-term) LS-C286383
LifeSpan Biosciences
catalog: LS-C286383
domestic rabbit polyclonal
reactivity: human
application: immunohistochemistry, immunocytochemistry
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GTF2I / TFII I Antibody (aa985-998) LS-C286521
LifeSpan Biosciences
catalog: LS-C286521
domestic rabbit polyclonal
reactivity: human
application: immunohistochemistry, immunocytochemistry
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GTF2I / TFII I Antibody (aa950-C-term) LS-C286961
LifeSpan Biosciences
catalog: LS-C286961
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry
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GTF2I / TFII I Antibody (aa935-985) LS-C288140
LifeSpan Biosciences
catalog: LS-C288140
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunoprecipitation
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GTF2I / TFII I Antibody (aa985-998) LS-C288145
LifeSpan Biosciences
catalog: LS-C288145
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunoprecipitation
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GTF2I / TFII I Antibody LS-C308151
LifeSpan Biosciences
catalog: LS-C308151
mouse polyclonal
reactivity: human
application: western blot, immunocytochemistry
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GTF2I / TFII I Antibody LS-C310563
LifeSpan Biosciences
catalog: LS-C310563
domestic rabbit polyclonal
reactivity: human
application: immunoprecipitation
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IHC-plus™ GTF2I / TFII I Antibody (aa956-985) LS-B11236
LifeSpan Biosciences
catalog: LS-B11236
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section

Anti-GTF2I / TFII I antibody IHC staining of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Western blot of lysates from A431, HeLa cell line (from left to right), using GTF2I Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.

Fluorescent image of A549 cell stained with GTF2I Antibody. A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with GTF2I primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). GTF2I immunoreactivity is localized to Nucleus significantly.
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GTF2I / TFII I Antibody (aa281-310) LS-C321832
LifeSpan Biosciences
catalog: LS-C321832
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA
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GTF2I / TFII I Antibody (aa956-985) LS-C328878
LifeSpan Biosciences
catalog: LS-C328878
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA
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GTF2I / TFII I Antibody LS-C331030
LifeSpan Biosciences
catalog: LS-C331030
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry

Western blot analysis of extracts of 293 cells, using GTF2I antibody. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.
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GTF2I / TFII I Antibody (clone 354C4a) LS-C342817
LifeSpan Biosciences
catalog: LS-C342817
mouse monoclonal (354C4a)
reactivity: human
application: immunocytochemistry
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IHC-plus™ GTF2I / TFII I Antibody (clone 3E2) LS-B12789
LifeSpan Biosciences
catalog: LS-B12789
mouse monoclonal (3E2)
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

GTF2I monoclonal antibody (M01), clone 3E2. Western blot of GTF2I expression in human colon.

Immunofluorescence of monoclonal antibody to GTF2I on HeLa cell. [antibody concentration 10 ug/ml].

Detection limit for recombinant GST tagged GTF2I is approximately 0.03 ng/ml as a capture antibody.
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GTF2I / TFII I Antibody (190-270 aa) LS-C380916
LifeSpan Biosciences
catalog: LS-C380916
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, ELISA
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