CD274 / B7-H1 / PD-L1 Antibody (clone MIH5) LS-C107430
LifeSpan Biosciences
catalog: LS-C107430
rat monoclonal (MIH5)
reactivity: human, mouse
application: immunohistochemistry, flow cytometry, immunohistochemistry - frozen section
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CD274 / B7-H1 / PD-L1 Antibody (clone OTI2C7) LS-C338364
LifeSpan Biosciences
catalog: LS-C338364
mouse monoclonal (OTI2C7)
reactivity: human
application: western blot, immunocytochemistry
citations: 2

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD274 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD274.

Anti-CD274 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CD274.
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CD274 / B7-H1 / PD-L1 Antibody LS-C746930
LifeSpan Biosciences
catalog: LS-C746930
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry
citations: 1
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CD274 / B7-H1 / PD-L1 Antibody (clone MIH5, PE) LS-C106539
LifeSpan Biosciences
catalog: LS-C106539
rat monoclonal (MIH5)
reactivity: human, mouse
conjugate: PE
application: flow cytometry
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CD274 / B7-H1 / PD-L1 Antibody (clone ANC6H1) LS-C134636
LifeSpan Biosciences
catalog: LS-C134636
mouse monoclonal (ANC6H1)
reactivity: human
application: ELISA, flow cytometry

Flow cytometry of B7-H1 / PD-L1 / CD274 antibody
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CD274 / B7-H1 / PD-L1 Antibody (clone ANC6H1, Biotin) LS-C134637
LifeSpan Biosciences
catalog: LS-C134637
mouse monoclonal (ANC6H1)
reactivity: human
conjugate: biotin
application: ELISA, flow cytometry

Flow cytometry of B7-H1 / PD-L1 / CD274 antibody This image was taken for the unconjugated form of this product. Other forms have not been tested.
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CD274 / B7-H1 / PD-L1 Antibody (clone ANC6H1, Azide-free) LS-C134638
LifeSpan Biosciences
catalog: LS-C134638
mouse monoclonal (ANC6H1)
reactivity: human
application: ELISA, flow cytometry

Flow cytometry of B7-H1 / PD-L1 / CD274 antibody This image was taken for the unmodified form of this product. Other forms have not been tested.
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CD274 / B7-H1 / PD-L1 Antibody (C-Terminus) LS-C169740
LifeSpan Biosciences
catalog: LS-C169740
domestic rabbit polyclonal
reactivity: human
application: western blot
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CD274 / B7-H1 / PD-L1 Antibody (clone MIH3) LS-C179364
LifeSpan Biosciences
catalog: LS-C179364
mouse monoclonal (MIH3)
reactivity: human
application: flow cytometry
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CD274 / B7-H1 / PD-L1 Antibody (clone MIH3, Biotin) LS-C179365
LifeSpan Biosciences
catalog: LS-C179365
mouse monoclonal (MIH3)
reactivity: human
conjugate: biotin
application: flow cytometry
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CD274 / B7-H1 / PD-L1 Antibody (clone 27A2) LS-C179481
LifeSpan Biosciences
catalog: LS-C179481
mouse monoclonal (27A2)
reactivity: human
application: immunohistochemistry, flow cytometry, immunohistochemistry - paraffin section
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CD274 / B7-H1 / PD-L1 Antibody (clone 27A2, PE) LS-C179482
LifeSpan Biosciences
catalog: LS-C179482
mouse monoclonal (27A2)
reactivity: human
conjugate: PE
application: flow cytometry
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CD274 / B7-H1 / PD-L1 Antibody (aa19-238, clone 12K56, APC) LS-C182804
LifeSpan Biosciences
catalog: LS-C182804
mouse monoclonal (12K56)
reactivity: human
conjugate: APC
application: flow cytometry
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CD274 / B7-H1 / PD-L1 Antibody (aa166-176) LS-C54629
LifeSpan Biosciences
catalog: LS-C54629
domestic goat polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

CD274 / B7-H1 / PD-L1 antibody (0.3µg/ml) staining of Human Heart (A) lysate + Blocking peptide (B) (35µg protein in RIPA buffer). Detected by chemiluminescence.

CD274 / B7-H1 / PD-L1 antibody flow cytometric analysis of paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

CD274 / B7-H1 / PD-L1 antibody immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
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CD274 / B7-H1 / PD-L1 Antibody (aa261-290, APC) LS-C233636
LifeSpan Biosciences
catalog: LS-C233636
domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA
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CD274 / B7-H1 / PD-L1 Antibody (aa261-290, Biotin) LS-C233637
LifeSpan Biosciences
catalog: LS-C233637
domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA
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CD274 / B7-H1 / PD-L1 Antibody (aa261-290, FITC) LS-C233640
LifeSpan Biosciences
catalog: LS-C233640
domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA
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CD274 / B7-H1 / PD-L1 Antibody (aa261-290, PE) LS-C233641
LifeSpan Biosciences
catalog: LS-C233641
domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA
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CD274 / B7-H1 / PD-L1 Antibody (aa261-290, AP) LS-C246999
LifeSpan Biosciences
catalog: LS-C246999
domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA
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CD274 / B7-H1 / PD-L1 Antibody (aa261-290, Azide-free, HRP) LS-C249630
LifeSpan Biosciences
catalog: LS-C249630
domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA
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IHC-plus™ CD274 / B7-H1 / PD-L1 Antibody (aa261-290) LS-B10562
LifeSpan Biosciences
catalog: LS-B10562
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Anti-B7-H1 / PD-L1 / CD274 antibody IHC staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.

CD274 Antibody western blot of NCI-H460 cell line lysates (35 ug/lane). The CD274 antibody detected the CD274 protein (arrow).
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CD274 / B7-H1 / PD-L1 Antibody (aa261-290) LS-C320169
LifeSpan Biosciences
catalog: LS-C320169
domestic rabbit polyclonal
reactivity: human
application: western blot
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CD274 / B7-H1 / PD-L1 Antibody LS-C331610
LifeSpan Biosciences
catalog: LS-C331610
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

Western blot analysis of extracts of various cell lines, using CD274 antibody at 1:3000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90s.
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CD274 / B7-H1 / PD-L1 Antibody (clone OTI1B12) LS-C338359
LifeSpan Biosciences
catalog: LS-C338359
mouse monoclonal (OTI1B12)
reactivity: human
application: western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD274 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD274.
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CD274 / B7-H1 / PD-L1 Antibody (clone OTI4D4) LS-C338366
LifeSpan Biosciences
catalog: LS-C338366
mouse monoclonal (OTI4D4)
reactivity: human
application: western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD274 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD274.
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CD274 / B7-H1 / PD-L1 Antibody (clone OTI2E3) LS-C338370
LifeSpan Biosciences
catalog: LS-C338370
mouse monoclonal (OTI2E3)
reactivity: human
application: western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD274 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD274.
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CD274 / B7-H1 / PD-L1 Antibody (clone OTI2B12) LS-C338375
LifeSpan Biosciences
catalog: LS-C338375
mouse monoclonal (OTI2B12)
reactivity: human
application: western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD274 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD274.
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CD274 / B7-H1 / PD-L1 Antibody (aa45-290) LS-C344014
LifeSpan Biosciences
catalog: LS-C344014
domestic rabbit polyclonal
reactivity: human
application: western blot

PD-L1 antibody Western blot. All lanes: Anti PDL1 at 0.5 ug/ml. WB: Recombinant Human PDL1 Protein 0.5ng. Predicted band size: 38 kD. Observed band size: 38 kD.
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CD274 / B7-H1 / PD-L1 Antibody (clone RBT-PDL1, Ready-to-Use) LS-C348566
LifeSpan Biosciences
catalog: LS-C348566
domestic rabbit recombinant (RBT-PDL1)
reactivity: human
application: immunohistochemistry - paraffin section, immunohistochemistry - frozen section

IHC of HPV on FFPE LSIL of the Cervix. This image was taken for the unmodified form of this product. Other forms have not been tested.
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CD274 / B7-H1 / PD-L1 Antibody (clone RBT-PDL1) LS-C348568
LifeSpan Biosciences
catalog: LS-C348568
domestic rabbit recombinant (RBT-PDL1)
reactivity: human
application: immunohistochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

IHC of HPV on FFPE LSIL of the Cervix.
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CD274 / B7-H1 / PD-L1 Antibody (Internal) LS-C354589
LifeSpan Biosciences
catalog: LS-C354589
domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot analysis of CD274 expression in HEK293T (A); Raw264.7 (B); H9C2 (C) whole cell lysates.
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CD274 / B7-H1 / PD-L1 Antibody (clone ANC6H1, RPE) LS-C357637
LifeSpan Biosciences
catalog: LS-C357637
mouse monoclonal (ANC6H1)
reactivity: human
conjugate: RPE
application: ELISA, flow cytometry

Flow cytometry of B7-H1 / PD-L1 / CD274 antibody This image was taken for the unconjugated form of this product. Other forms have not been tested.
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CD274 / B7-H1 / PD-L1 Antibody (clone ANC6H1, FITC) LS-C357681
LifeSpan Biosciences
catalog: LS-C357681
mouse monoclonal (ANC6H1)
reactivity: human
conjugate: FITC
application: ELISA, flow cytometry

Flow cytometry of B7-H1 / PD-L1 / CD274 antibody This image was taken for the unconjugated form of this product. Other forms have not been tested.
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IHC-plus™ CD274 / B7-H1 / PD-L1 Antibody LS-B12923
LifeSpan Biosciences
catalog: LS-B12923
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)
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CD274 / B7-H1 / PD-L1 Antibody (aa34-241) LS-C374016
LifeSpan Biosciences
catalog: LS-C374016
domestic rabbit polyclonal
reactivity: human, rat
application: western blot

Western blot of CD274 / B7-H1 / PD-L1 antibody.

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CD274 / B7-H1 / PD-L1 Antibody (aa19-238) LS-C379803
LifeSpan Biosciences
catalog: LS-C379803
domestic rabbit polyclonal
reactivity: human
application: ELISA, immunohistochemistry, immunohistochemistry - paraffin section

Immunohistochemistry of paraffin-embedded human rectum using antibody 1:100 dilution.
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