IHC-plus™ PYCARD / ASC / TMS1 Antibody LS-B1393
LifeSpan Biosciences
catalog: LS-B1393
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Anti-PYCARD antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Immunocytochemistry of ASC in HL60 cells with ASC antibody at 5 ug/ml.
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PYCARD / ASC / TMS1 Antibody (clone OTI3E9) LS-C175123
LifeSpan Biosciences
catalog: LS-C175123
mouse monoclonal (OTI3E9)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section
citations: 1

Western blot of extracts (35 ug) from 9 different cell lines by using g anti-PYCARD monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

IHC of paraffin-embedded Human colon tissue using anti-PYCARD mouse monoclonal antibody.

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PYCARD antibody, and then analyzed by flow cytometry.
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PYCARD / ASC / TMS1 Antibody (aa71-120) LS-C30594
LifeSpan Biosciences
catalog: LS-C30594
domestic rabbit polyclonal
reactivity: human, mouse
application: western blot
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PYCARD / ASC / TMS1 Antibody LS-C48292
LifeSpan Biosciences
catalog: LS-C48292
domestic rabbit polyclonal
reactivity: human
application: western blot
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IHC-plus™ PYCARD / ASC / TMS1 Antibody (aa182-195) LS-B4431
LifeSpan Biosciences
catalog: LS-B4431
domestic goat polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

PYCARD / ASC / TMS1 antibody (2µg/ml) staining of U937 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-PYCARD antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.
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IHC-plus™ PYCARD / ASC / TMS1 Antibody (aa31-46) LS-B4826
LifeSpan Biosciences
catalog: LS-B4826
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Anti-PYCARD antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
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PYCARD / ASC / TMS1 Antibody (aa121-170) LS-C135042
LifeSpan Biosciences
catalog: LS-C135042
domestic rabbit polyclonal
reactivity: human
application: western blot
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PYCARD / ASC / TMS1 Antibody LS-C155196
LifeSpan Biosciences
catalog: LS-C155196
domestic rabbit polyclonal
reactivity: human
application: western blot

Sample (30 ug of whole cell lysate) A: HeLa 12% SDS PAGE PYCARD antibody diluted at 1:1000
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PYCARD / ASC / TMS1 Antibody (clone OTI1H6) LS-C174609
LifeSpan Biosciences
catalog: LS-C174609
mouse monoclonal (OTI1H6)
reactivity: human
application: western blot, flow cytometry

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PYCARD (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PYCARD.

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PYCARD antibody, and then analyzed by flow cytometry.
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PYCARD / ASC / TMS1 Antibody (Glu13) LS-C176873
LifeSpan Biosciences
catalog: LS-C176873
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot of ASC (E13) pAb in extracts from RAW264.7 cells.

IHC of ASC (E13) pAb in paraffin-embedded human lung carcinoma tissue.
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PYCARD / ASC / TMS1 Antibody (clone 23-4) LS-C179356
LifeSpan Biosciences
catalog: LS-C179356
mouse monoclonal (23-4)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation
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PYCARD / ASC / TMS1 Antibody LS-C180180
LifeSpan Biosciences
catalog: LS-C180180
domestic rabbit polyclonal
reactivity: human
application: immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
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PYCARD / ASC / TMS1 Antibody (Biotin) LS-C180934
LifeSpan Biosciences
catalog: LS-C180934
domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Human Brain, Cerebellum: Formalin-Fixed, Paraffin-Embedded (FFPE). This image was taken for the unconjugated form of this product. Other forms have not been tested.

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PYCARD / ASC / TMS1 Antibody (DY650) LS-C180935
LifeSpan Biosciences
catalog: LS-C180935
domestic rabbit polyclonal
reactivity: human
application: immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Human Brain, Cerebellum: Formalin-Fixed, Paraffin-Embedded (FFPE). This image was taken for the unconjugated form of this product. Other forms have not been tested.

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PYCARD / ASC / TMS1 Antibody (DY488) LS-C180936
LifeSpan Biosciences
catalog: LS-C180936
domestic rabbit polyclonal
reactivity: human
application: immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Human Brain, Cerebellum: Formalin-Fixed, Paraffin-Embedded (FFPE). This image was taken for the unconjugated form of this product. Other forms have not been tested.

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PYCARD / ASC / TMS1 Antibody (DY550) LS-C180938
LifeSpan Biosciences
catalog: LS-C180938
domestic rabbit polyclonal
reactivity: human
application: immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Human Brain, Cerebellum: Formalin-Fixed, Paraffin-Embedded (FFPE). This image was taken for the unconjugated form of this product. Other forms have not been tested.

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PYCARD / ASC / TMS1 Antibody (Biotin) LS-C180939
LifeSpan Biosciences
catalog: LS-C180939
domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
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PYCARD / ASC / TMS1 Antibody (DY650) LS-C180940
LifeSpan Biosciences
catalog: LS-C180940
domestic rabbit polyclonal
reactivity: human
application: immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
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PYCARD / ASC / TMS1 Antibody (DY488) LS-C180941
LifeSpan Biosciences
catalog: LS-C180941
domestic rabbit polyclonal
reactivity: human
application: immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
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PYCARD / ASC / TMS1 Antibody (DY550) LS-C180943
LifeSpan Biosciences
catalog: LS-C180943
domestic rabbit polyclonal
reactivity: human
application: immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
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PYCARD / ASC / TMS1 Antibody (aa100-111) LS-C186593
LifeSpan Biosciences
catalog: LS-C186593
domestic goat polyclonal
reactivity: human, mouse
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PYCARD / ASC / TMS1 Antibody (aa51-63) LS-C186594
LifeSpan Biosciences
catalog: LS-C186594
domestic goat polyclonal
reactivity: human
application: western blot

PYCARD antibody (2 ug/ml) staining of Mouse (A) and Rat (B) Spleen lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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PYCARD / ASC / TMS1 Antibody (aa129-158) LS-C162336
LifeSpan Biosciences
catalog: LS-C162336
domestic rabbit polyclonal
reactivity: human
application: western blot

PYCARD Antibody western blot of MCF-7 cell line lysates (35 ug/lane). The PYCARD antibody detected the PYCARD protein (arrow).
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IHC-plus™ PYCARD / ASC / TMS1 Antibody (aa10-59) LS-B9615
LifeSpan Biosciences
catalog: LS-B9615
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Anti-PYCARD / ASC antibody IHC staining of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.

Western blot analysis of lysates from RAW264.7 cells, using ASC Antibody. The lane on the right is blocked with the synthesized peptide.
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PYCARD / ASC / TMS1 Antibody (aa129-158, FITC) LS-C284917
LifeSpan Biosciences
catalog: LS-C284917
domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA
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PYCARD / ASC / TMS1 Antibody (aa129-158, PE) LS-C284922
LifeSpan Biosciences
catalog: LS-C284922
domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA
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PYCARD / ASC / TMS1 Antibody (aa129-158) LS-C324443
LifeSpan Biosciences
catalog: LS-C324443
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA
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PYCARD / ASC / TMS1 Antibody LS-C331318
LifeSpan Biosciences
catalog: LS-C331318
domestic rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry

Western blot analysis of extracts of various cell lines, using PYCARD antibody. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.

Immunofluorescence analysis of HeLa cells using PYCARD antibody.
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IHC-plus™ PYCARD / ASC / TMS1 Antibody LS-B11792
LifeSpan Biosciences
catalog: LS-B11792
domestic rabbit polyclonal
reactivity: human
application: immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Human Brain, Cerebellum: Formalin-Fixed, Paraffin-Embedded (FFPE). This image was taken for the unconjugated form of this product. Other forms have not been tested.

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PYCARD / ASC / TMS1 Antibody (clone OTI1A2) LS-C336917
LifeSpan Biosciences
catalog: LS-C336917
mouse monoclonal (OTI1A2)
reactivity: human
application: western blot, flow cytometry

Western blot of extracts (35ug) from 9 different cell lines by using anti-PYCARD monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
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PYCARD / ASC / TMS1 Antibody (clone OTI2A7) LS-C336919
LifeSpan Biosciences
catalog: LS-C336919
mouse monoclonal (OTI2A7)
reactivity: human
application: western blot, immunohistochemistry, flow cytometry, immunohistochemistry - paraffin section

Western blot of extracts (35ug) from 9 different cell lines by using anti-PYCARD monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

IHC of paraffin-embedded Human colon tissue using anti-PYCARD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min).
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PYCARD / ASC / TMS1 Antibody LS-C358644
LifeSpan Biosciences
catalog: LS-C358644
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunoprecipitation
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PYCARD / ASC / TMS1 Antibody (10-90 aa, N-terminal) LS-C382166
LifeSpan Biosciences
catalog: LS-C382166
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry

Western blot of ASC antibody
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PYCARD / ASC / TMS1 Antibody (N-Terminus) LS-C413166
LifeSpan Biosciences
catalog: LS-C413166
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot analysis of ASC expression in HEK293T (A); Raw264.7 (B); mouse brain (C); rat brain (D) whole cell lysates.

Immunohistochemical analysis of ASC staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Immunofluorescent analysis of ASC staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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